DAFTAR ISI
JUDUL.................................................................................................................... i KATA PENGANTAR............................................................................................ ii DAFTAR ISI.......................................................................................................... iii BAB I PENDAHULUAN....................................................................................... 1 1.1 Latar Belakang................................................................................................. 1 1.2 Rumusan Masalah............................................................................................ 2 1.3 Batasan Masalah............................................................................................... 2 1.4 Tujuan Penelitian.............................................................................................. 2 1.5 Manfaat Penelitian............................................................................................ 3 BAB II TINJAUAN PUSTAKA............................................................................. 4 2.1 Landasan Teori.................................................................................................. 4 2.1.1 Morfologi Tanaman Suruhan................................................................ 4 2.1.2 Klasifikasi Tanaman Suruhan............................................................... 5 2.1.3 Nama Daerah........................................................................................ 5 2.1.4 Manfaat................................................................................................. 5 2.1.5 Kandungan Kimia................................................................................. 5 2.1.6 Pertumbuhan Tanaman Suruhan di Indonesia....................................... 2 2.2 Ekstraksi........................................................................................................... 6 2.2.1 Pengertian Ekstraksi............................................................................. 6 2.2.2 Proses Ekstraksi................................................................................... 6 .3 Antioksidan.................................................................................................... 12 2.3.1 Pengertian Antioksidan...................................................................... 12 2.3.2 Sumber Antioksidan........................................................................... 12 2.3.3 Fungsi Antioksidan............................................................................ 14 2.4 Radikal Bebas................................................................................................. 16 2.5 Uji Aktifitas Antioksidan dengan Metode DPPH.......................................... 17 iii 2.6 Spektrofotometer............................................................................................ 18 2.6.1 Pengertian Spektrofotometer............................................................ 18 2.6.2 Bagian – Bagian Spektrofotometer.................................................. 18 2.6.3 Prinsip Kerja Spektrofotometer........................................................ 19 2.6.4 Jenis – Jenis Spektrofotometer......................................................... 19 2.7 Vitamin C...................................................................................................... 21 2.8 Metode Experimental.................................................................................. 22 2.8.1 Pengertian Metode Experimental...................................................... 22 2.8.2 Karakteristik Penilaian Experimental............................................... 22 2.8.3 Tujuan Penelitian Experimental........................................................ 23 2.8.4 Syarat – Syarat Penelitian Experimental........................................... 24
2.8.5 Proses Penelitian Experimental......................................................... 24 BAB III METODE PENELITIAN....................................................................... 25 3.1 Jenis Penelitian........................................................................................... 25 3.2 Waktu dan Tempat Penelitian.................................................................... 25 3.3 Alat dan Bahan............................................................................................ 25 3.3.1 Alat Penelitian.................................................................................. 25 3.3.2 Bahan Penelitian.............................................................................. 25 3.4 Tahap Penelitian......................................................................................... 25 3.4.1 Determinasi Bahan.......................................................................... 25 3.4.2 Ekstraksi.......................................................................................... 26 3.5 Uji Antioksidan Secara ............................................................................. 26 Kuantitatif dengan Spektrofotometr UviVis 3.5.1 Pembuatan Larutan DPPH 125µM.................................................. 26 3.5.2 Preparasi Sampel dan Vitamin C..................................................... 26 3.5.3 Cara Kerja Sampel dan Vitamin C.................................................. 27 3.6 Skema Kerja................................................................................................ 28 3.6.1 Skema Kerja Pembuatan Ekstrak Daun Suruhan............................ 27 3.6.1 Skema Kerja Ekstrak Etanol 70%................................................... 28 Daun Suruhan dengan Metode DPPH iv