Anandabhairava Rasa

By-

Dr. Manojkumar Patil P.G.Scholar Dept Of Rasashastra

UNDER THE GUIDANCE OF Dr M.S.GavimathM.D Dr (Smt)P .P. Dindore. MD Dr R.S. Hiremath M.D

K.L.E’s Shri B M K Ayurveda Mahavidyalaya. Belgaum, Karnataka.

CONTENTS 

INTRODUCTION



OBJECTIVES



METHODOLOGY



RESULTS



DISCUSSION



CONCLUSION

INTRODUCTION 

Anandbhairava rasa is one among,mentioned for the cure of Jwaratisara.



Adopting the preparation by good manufacturing practice,optimum quality of drug can be prepared



Numerous diseases arise by various microorganisms,among infectious diarrhea is one where,E.Coli and S.Aureus are the main causative organisms.

OBJECTIVES 

Preparation of Anandabhairava rasa as per classical reference.



Physico-Chemical analysis of the Anandabhairava rasa.



Anti-microbial study of prepared standard formulation in comparison of two different market samples of Anandabhairava rasa to evaluate it’s sensitivity on micro-organisms.

METHODOLOGY It is in two groups A) Material and methods of Anandabhairava rasa.

B) Material and methods of Antimicrobial study

PREPARATION OF ANANDA BHAIRAVA RASA 

Equipment :Khalwayantra,Patra



Ingredients : 1. Shuddha Hingula 2. Shuddha Vatsanabha 3. Trikatu 4. Shuddha Tankana 5. Shuddha Gandhaka 6. Jambeera Swarasa



- 100 gms - 100 gms - 100 gms - 100 gms - 100 gms - 600 ml

Procedure : - All the ingredients are taken in khalwayantra in above mentioned proportion. - Jambeera swarasa is added to it. - Trituration is done carefully. - Bhavana is given for 6 Hrs. - Then gunja pramana vati are prepared. - Vatis are placed on a patra and allowed to shadow dry for one day. - Then collected and preserved in a plastic container.

Organoleptic features of Anandabhairava Rasa : Features Sl. No. 1.

Colour

Reddish orange

2.

Smell

Pleasant

3.

Touch

Fine powder

Quantitative Analysis of Raw drugs : Sl. N o

Samples Tests I

II

III

IV

V

VI

VII

-

-

1

Hg%

86.5% w/w

66.6% w/w

-

-

-

2.

S%

12.64% w/w

8.0% w/w

-

-

-

3.

Borax %

-

-

16.6% 17.55% w/w w/w

16.7% w/w

-

-

4.

Na %

-

-

3.8% w/w

3.8% w/w

-

-

I  Ashudda Hingula II Shudha Hingula III  Ashudda Tankana IV  Shudha Tankana

4.2% w/w

11.17% 10.48% w/w

V  Nirmaleekruta Tankana VI  Ashudha Gandhak VII  Shuddha Gandhak

Analysis of prepared Anandabhairava rasa Sl. N o.

Sampl e

Tests

Hg %

01.

VIII

12.3% w/w

S%

18.73% w/w

Free S % Nil

Ash value

Acid Insoluble Ash

15.0% w/w

1.47% w/w

VIII  Anadabharava Rasa

Borax

1.01% w/w

Fe as Fe2 O3 0.17% w/w

Volatile matter

15.61% w/w

Ca %

0.977% w/w

Phosphate as PO4

Nil

LOD at 110OC

12.38% w/w

Silica

K+

0.95% w/w

0.48% w/w

P

H

7.24

Method: The Antimicrobial study was designed in two different methods as :  Macro dilution broth susceptibility test.(M.B.S.T) for more accuracy  Cup- plate diffusion method.

1) Cup-plate diffusion method  Standard drug  Test organism  Preparation of test solution  Preparation of inoculum  Culture medium  Sterilization  Preparation of agar plate  Experimental procedure 2) Macro dilution Broth Susceptibility Test (M.B.S.T)  Preparation of stock solution  Experimental procedure

ANTIBACTERIAL AND ANTIFUNGAL SCREENING RESULTS

Results of cup-plate diffusion method Zone of inhibition in mm : Compound code

S. Aureus (+ ve)

E-coli (-ve)

Candida Albicans (Fungal )

Sl No. 0.1 mg/ml

0.5 mg/ml

0.1 mg/ml

0.5 mg/ml

0.1 mg/ml

0.5 mg/ml

01.

Standard (prepared)

12 (0.66)

11 (0.61)

14 (0.64)

14 (0.68)

1 (0.05)

1 (0.05)

02.

Market – I

8 (0.44)

10 (0.55)

11 (0.50)

13 (0.59)

0 (–)

2 (0.12)

03.

Market – II

6 (0.33)

11 (0.61)

12 (0.55)

13 (0.59)

1 (0.05)

1 (0.05)

Results of Standard Allopathic Drug on organisms : Standard drugs

S. Aureus

E.Coli

Sl. No.

Candida Albicans

01.

Oflaxacine (2 mcg/ml)

20

22

-

02.

Ampicillin (10 mcg/ml)

18

19

-

03.

Gresiofulvin

-

-

17

Macrodilution Broth Susceptibility Method Result of STAPHYLOCOCCUS AUREUS :

Sl. No.

10 mg/ ml

5 mg/ml

2.5 mg/ ml

1.25 mg/ ml

0.612 mg/ ml

0.3 mg/ ml

0.15 mg/ ml

MIC Valu e

Mkt I

S

R

R

R

R

R

R

10

Mkt II

S

R

R

R

R

R

R

10

III

S

S

S

S

R

R

R

1.25

2.5 mg/ ml

1.25 mg/ ml

Result of ESHERICHIA COLI : Sl. No.

10 mg/ ml

5 mg/ml

Mkt I

S

S

R

R

R

R

R

5

Mkt II

S

S

S

R

R

R

R

2.5

III

S

S

S

R

R

R

R

2.5

Mkt. I  Market Sample I Mkt II Market sample II

0.612 mg/ ml

0.3 mg/ ml

III  Standard drug

0.15 mg/ ml

MIC Valu e

Zone of inhibition

Macro dilution Broth Susceptibility Test Method

DISCUSSION 1st Method 

All the three show activity on organisms.



Two concentration of 0.1mg/ml & 0.5 mg/ml were studied



Zone of inhibition of prepared drug is little more, especially on E.coli at 14mm



Anti fungal activity on C.Albicans was very minimal

Cont… 2nd Method 

Studied in 7 different concentration



Prepared drug shown activity for S.Aureus at 1.25 mg/ml conc.where as both market drug shown activity at 10 mg/ml



Prepared drug shown activity on E.Coli at 2.5 mg/ml conc. Market –II also shown at the same, where as market –I shown activity at 5 mg/ml conc.



Still drug concentration to be improved as compared standard allopathic drugs

CONCLUSION 

Anandabhairava rasa is economic, easy for preparation (raw drugs are easily available)



Qualitative & Quantitative values are within the range of standard values



Overall effect of Anandabhairava rasa on micro-organisms is sensitive



More significant activity on S.Aureus.



Prepared drug is more potent on micro-organisms as compared to market samples

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