Vaccine Production

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Vaccine Production Using our paper-carrier based "Current" perfusion culture bioreactor, we are able to culture cells at extremely high densities, replacing classical roller bottle and current microcarrier systems. Because of this fact, we can use our "Current" perfusion bioreactor to culture human and animal vaccine producing cells (such as VERO MDCK, etc...) very rapidly to counter global disease outbreaks. Furthermore, we have developed a scalable seeding process that has simplified the trypsin-digest/seeding process. We have tested cells from several species (see data below) with remarkable results, improving the industrial manufacture of vaccines.

"Current" Paper­carrier Perfusion Bioreactor Cell Culture Data  (cell density/gram paper-carrier by paper-carrier total weight) for vaccine production

Cell line name

5L perfusion bioreactor (cell#/gram×paper-carrier total weight 150 grams)

50L perfusion bioreactor (cell#/gram×paper-carrier total weight 1200 grams)

150L perfusion bioreactor (cell#/gram×paper-carrier total weight 3600 grams)

VERO (human)

6.0±1.2×108cells/g×150g

6.5±2.1×108cells/g×1200g

Pending

MDCK (human)

5.0±1.0×108cells/g×150g

Pending

Pending

2BS (diploid)

3.0×108cells/g×150g

Pending

Pending

Mark145 (animal)

3.5±0.8×108cells/g×150g

Pending

Pending

ST1 (animal)

4.0±1.0×108cells/g×150g

Pending

Pending

DF-1 (chicken)

2.5±0.3×108cells/g×150g

Pending

Pending

CIK (fish)

1.0±0.2×108cells/g×150g

Pending

Pending

EPC (fish)

1.2±0.3×108cells/g×150g

Pending

Pending

Conclusions:

1) A 50L paper-carrier perfusion bioreactor equals a production plant of 1200 large roller bottles and is perfect for larger-scale animal and human vaccine production, including chicken and fish. It is also great for rapid vaccine production of a deadly virus outbreak (such as SARS and bird flu outbreaks) after the virus DNA extracted. 2) Single-use and low cost. Advantages: 1) Due to extremely high cell density, cell-cell growth support reaches the peak greatly for low-growth rate vaccine production cell lines; 2) Compared with roller-bottles and micro-carrier methods, seeding method (cell wash & trypsin-digest) for scale up is greatly simplified. 3) DO,pH,temperature control and cell line-specific medium optimization.

Human & Animal Vaccine Process Development

Taking advantage of AmProtein's high-density cell culture perfusion system we have developed MDCK, VERO, Chicken and Fish cell process. These processes are for sale together with the perfusion system to interested vaccine manufacturers. AmProtein also welcomes collaborations to work on newly discovered vaccines and push them towards commercialization or grant applications towards final commercialization.

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