Sab Q1 Email)

2009 New Product Announcement 10% Promotional Discount Pathway-focused Gene Expression Profiling

RT2 Nano PreAMPTM Kits NEW! Enabling the Analysis of 1ng Total RNA on RT2 Profiler PCR Array

PCR Arrays

Over 100 pathways available. The real-time qPCR expert. inflammation

- Enables the gene expression analysis on up to 4 biology or diseasefocused pathways using as little as 1 ng total RNA. - Employs proprietary amplification process to increase the amount of respective cDNA for subsequent RT2 Profiler™ PCR Arrays analysis. The samples that are enabled for real-time PCR Arrays include: ● Fine needle biopsy (FNB) samples. ● Laser captured microdissection (LCM) samples. ● Stem cell clusters or embryoid bodies. ● Fluorescence-Activated Cell Sorter (FACS) generated cells.

stem cell cell cycle apoptosis neuroscience

See complete list of pathways: http://www.sabiosciences.com/ArrayList.php?pline=PCRArray SABiosciences is now Podcasting! Download a pre-recorded seminar. Watch and listen on your iPod or on your computer on your own time - on the train or the bus, at home, during lunch, or while working in the lab. Listen to the overview of PCR array technology here: http://www.sabiosciences.com/seminarlist.php

DNA Methylation

Cell-based Assays

Methyl-ProfilerTM PCR Array NEW! Fast and Accurate Detection of CpG Island

New 11 Luciferase Reporter Assays Added

Firefly Luciferase

Tandem TRE

Renilla Luciferase

● DUAL-LUCIFERASE FORMAT: Exceptional sensitivity,

CMV

Monster GFP

Methly-Profiler PCR Arrays

- Ideal for analysis of: • siRNA/shRNA/miRNA phenotypes • biological responses to chemical compounds • mechanisms of action of proteins, peptides and ligands - Transfection-ready format with your favourite cell lines reproducibility, specificity, and signal to noise ratio ● GFP FORMAT: Single cell resolution; Real-time live cell quantification

Tandem TRE

Cat # Catalog # MeAH-011

Human Breast Cancer Human Gastric Cancer MeAH-021 Human Liver Cancer MeAH-031 MeAH-041 Human Lung Cancer Human Prostate Cancer MeAH-051 http://www.sabiosciences.com/dna_methylation.php

Components

Transcription factor-specific reporter, negative control, positive control. Supplied material provides sufficient reporter for 500 assays.

Popular Cignal Reporter Assays

CignalTM Reporter Assays

- Replaces the low-throughput bisulfite-based methods with a simple restriction digest and qPCR. - Available as individual assays for every CpG island in the human genome. - Features: ● Simpler Protocol: Restriction digests instead of multi-step bisulfite treatment & purification ● Faster: Higher Gene & Sample Throughput; 4 to 6 h digestion instead of traditional overnight procedures ● No Loss + Amplification = Less Material Needed; 1 μg genomic DNA profiles 24 genes instead of only 5 genes

Panels

LMD samples

See how it works here : http://www.sabiosciences.com/reporterassays.php

Chromatin IP

TRANSCRIPTION FACTOR

LUCIFERASE

Androgen Receptor Nrf2 & Nrf1 C/EBP CREB E2F/DP1 p53 Early Growth Response 1 Estrogen Receptor (ER) SP1 Glucocorticoid Receptor (GR) HSF Hypoxia-inducible Factor-1 (HIF-1) STAT1/STAT2 STAT1/STAT1 MEF2 Elk-1/SRF AP-1 Myc/Max NFκB RBP-Jk FOXO NFAT PPAR Retinoic Acid Receptor (RAR) STAT3 Oct4 SMAD2/SMAD3/SMAD4 Vit D Receptor TCF/LEF

CCS-1019L CCS-5020L CCS-001L CCS-002L CCS-003L CCS-004L CCS-8021L CCS-005L CCS-6027L CCS-006L CCS-4023L CCS-007L CCS-008L CCS-009L CCS-7024L CCS-010L CCS-011L CCS-012L CCS-013L CCS-014L CCS-1022L CCS-015L CCS-3026L CCS-016L CCS-9028L CCS-0025L CCS-017L CCS-2029L CCS-018L

Antibody Name

- Available for TFs, modified or unmodified histones and other nuclear factors. - Guaranteed to yield the highest signal-to-noise ratios when combined with the ChampionChIP One-Day Kit. ● Convenience: Experimentally validated, ready-to-use ChIP-grade antibodies ● Sensitivity and Specificity: Qualified ChIP-grade antibodies with high efficiency and low background ● Reliability: Appropriate positive and negative control real-time qPCR primers are included

RT2 miRNA PCR Array

DECODE: ChIP-qPCR Assay Search Portal Supported by SABiosciences' proprietary database known as DECODE (DECipherment Of DNA Elements), this search portal reports binding sites for over 200 different transcription factors in any human promoter. Use this search portal to find PCR primers for your favorite transcription factors' binding sites especially if you DO NOT know their location relative to the Transcription Starting Site (TSS) of your human genes of interest.

an & Hum nome G e s e Mou ailable Av

- One-shot analysis of 88 or 376 pathway-focused miRNA sequences - Single nucleotide mismatch discrimination - Distinction between mature miRNA and their precursors Sensitivity: As little as 0.5 µg total RNA needed Multi-Sequence Flexibility: Analyze up to 376 sequences simultaneously ● Simplicity: As easy as a real-time PCR experiment ● ●

shRNA 2

CCS-017G CCS-018G CCS-002G CCS-010G CCS-010G CCS-011G CCS-002G CCS-013G CCS-017G CCS-018G CCS-002G CCS-017G CCS-018G

GA-111 GA-112

ANTIBODY NAME

MODIFICATIONS

H3ac H3K9ac H4ac H3K4me1 H3K4me2 H3K4me3 H3K9me1 H3K9me2 H3K9me3 H3K27me3 H3K36me3 H3K79me3 H3K20me3 H3

Acetylation Acetylation Acetylation Methylation Methylation Methylation Methylation Methylation Methylation Methylation Methylation Methylation Methylation Unmodified Control

CATALOGUE NUMBER GAH-7201 GAH-1209 GAH-0202 GAH-5207 GAH-3203 GAH-8208 GAH-5210 GAH-8211 GAH-6204 GAH-9205 GAH-1212 GAH-4213 GAH-7214 GAH-2206

Learn more about our ChIP system here: http://www.sabiosciences.com/chipqpcr.php

http://www.sabiosciences.com/miRNAsearch.php

Pathway CentralTM FREE Editable Pathway Map in Powerpoint Format - A pathway study resource for you to find pathway reviews and presentation-ready pathway maps. NEW FEATURES: D A O L • Download free pathway maps in PowerPoint. DOWN Ready-to-use for your lab meeting! T

R BLE FO AVAILA OWERPOIN IN P

- Available miRNA PCR Arrays: Cancer Cell Differentiation & Development Whole Genome miFinder

- Three easy ways to navigate in the pathway central • Browse based on your research areas. • Search with key words. • View the complete list of pathways

http://www.sabiosciences.com/pathwaycentral.php You will love it!

Genomax Technologies Pte Ltd

CCS-010G

Search for your miRNA qPCR assay specific for sequences regulating your gene of interest. Assays are available for every over 400 majority miRNA sequences. Each assay is experimentally validated to amplify a single amplicon with uniform PCR efficiency.

miRNA Profiling with PCR Array Technology

DECODE TSS Search Portal

CCS-013G CCS-002G CCS-018G CCS-002G

miRNA qPCR Assay Search Portal

miRNA Profiling

ChampionChIP™ qPCR Primer

GFP

Catalogue Number

Polymerase II P53

ChampionChIP™ Antibody Kit NEW! Validated High-quality ChIP-grade Abs

ChampionChIP PCR System includes: ● ChampionChIP™ Antibody Kits- Validated ChIP-grade antibodies with positive/negative control primers ● ChampionChIP™ One-Day Kit- Simple and Robust ChIP kit ● ChampionChIP™ Real-Time PCR Primers- Analyze any transcription factor and histone binding to chromatin via qPCR

Stem Cell

ChampionChIP™ Transcription Factor Ab Kits

- Designed for performing chromatin immunoprecipitation (ChIP) using your carefully chosen antibodies to specifically enrich genomic DNA fragments that bind in vivo to transcription factors, modified histones or other nuclear factors of interest. ● Speed & Ease: Simplified protocol easily obtains ChIP DNA from cells in just 6 hours ● Universality: General kit compatible with any ChIP-Grade antibody ● Reliability: High-yield and high-quality DNA for real-time PCR and highly reproducible enrichment

- Complete platform for the analysis of in vivo protein-DNA interactions using ChIP and qPCR detection. - Simple and robust one-day preparation kit with a genome-wide qPCR primer design to quickly deliver reliable and biologically relevant results.

FACS samples

PATHWAY

ChampionChIP™ One-Day Kit Fast ChIP with Reliable Results

ChampionChIP™ PCR System

Please visit : http://www.sabiosciences.com/chipqpcrsearch.php

FNB samples

Androgen Antioxidant Response C/EBP cAMP/PKA Cell Cycle p53/DNA Damage Early Growth Response Estrogen Receptor GC Box Binding TF Glucocorticoid Receptor Heat Shock Hypoxia Type I Interferon Interferon Gamma MADS Box MAPK/ERK MAPK/JNK c-myc NFκB Notch PI3K/Akt Pathway PKC/Ca++ PPAR Retinoic Acid Receptor STAT3 Stem Cell Marker TGFβ VDRE Wnt

Modified Histone Ab Kits

cancer

(65) 6777 1886

[email protected]

To benefit from these offers, please make sure you quote SAB09 on your order. Prices are valid until 30/4/2009 and are not compatible with other offers, special prices or discounts.