Custom Antibody, Protein, Peptide Service

3.1. Polyclonal, monoclonal and recombinant antibody Service Items Polyclonal antibody

Timeline

Criteria

10-12 Weeks

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Design, synthesize and conjugate peptide or recombinant protein Immunization of Animals(Mouse, Rabbit, Sheep, et.al) Fixing amount of immune sera Generated antibody recognizes immunogen in ELISA assay ELISA titer is up to 1:10000 Antibody purification by affinity column upon request

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Design, synthesize and conjugate peptide or recombinant protein Immunization of two mice, fusion, screening, subclone and initial characterization

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Monoclonal antibody 4-5 months

With up to three monoclones and their supernatant for confirming d detection • Generated antibody recognizes immunogen in ELISA assay • Antibody purification by affinity column(5mg) • Customer clone ownership agreement Design, synthesize and biotinylated peptide or recombinant protein Selecting, screening, and initial characterization with up to five ELISA positive clones 10 ml of Phage and soluble antibody for customer confirmig detection Antibody gene sequence and analysis for specific clone Antibody purification by affinity column may be obtained upon request Customer clone ownership agreement

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Recombinant antibody 6-8 weeks

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Peptide & Peptide Synthesis Custom Recombinant Protein Custom Antibody Production

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Describe your location by landmark or area of town.

3.2. Antibody Engineering Followings are suitable for modify your antibody: Well characterized non-human antibody Unique monoclonal antibody but it is produced from unstable hybridoma Want to change your antibody format Improve antibody functions Convert monoclonal antibody to scFv, and convert scFv to whole IgG

3.3. Proteomics Antibody Our platforms are to help you explore the entire human proteome using protein-specific antibody with high-affinity. By combining of antibody extensive generation with comprehensive antibody characterization, we will help you reach your aim in proteomics research and target discovery. Screening protein-protein interactions using available antibody-based array Parallel, multiplexed, miniaturized, high-throughput proteomic analysis by array Individual protein detection, protein expression profiling, and interactions between proteins and ligands, antibodies, or other proteins In antibody generation, that is included as traditional antibody generation as well as high-throughput recombinant antibody screening In antibody function, that is extended to combination of our available arrays (peptide, protein and tissue) with capturing of specific proteins except doing routine immunological analysis

Custom Peptide, Protein, Antibody Service and Production RayBiotech, Inc. - The Protein Array Pioneer Company. 3607 Parkway Lane, suite 200, Norcross, GA 30092 Website: www.raybiotech.com Phone: 770-729-2992, 1-888-494-8555 E-mail: [email protected]

Custom Peptide, Protein, Antibody Service and Production 1. Custom Peptide production 1.1. Peptide synthesis Custom peptide synthesis: All kinds of peptides, crude, purified, modification, even rare amino acids. Pre-made peptides: hundreds of pre-made peptides are in stock. Cost effective: As with our custom peptide service, our pricing is always competitive. Price(US $)/residue

Purity

Quality(mg)

Crude

20-50

7.8-10.2

>70%

20-50

12.8-15

>80%

20-50

16.9-19.9

>95%

1-5

18.6-21.9

(6-30 mer peptide)

* Note: Other purity and quality are available upon request.

1.2. Leading peptide screen Peptide screening service: Selecting and identifying potential peptide by using random peptide library. Epitope mapping: defining critical binding region of functional antibody, including neutralizing antibody. A final format peptide will be provided in synthetic or expressed form for customer’s further testing in cell or animal model.

2. Recombinant Protein Production

3. Custom Antibody Service

Our Custom Protein Service offers all aspects of protein expression, production, purification, and development, from gene to protein including: Cloning; Subcloning; Generation of plasmid DNA; Multiprotein Complex Expressions; Optimization of expression conditions; Small, mini- or large-scale expression and affinity purification; Purity evaluation and determination of concentration; Lyophilization of protein.

Our Custom Protein Service offers all aspects of protein expression, production, purification, and development, from gene to protein including: Cloning; Subcloning; Generation of plasmid DNA; Multiprotein Complex Expressions; Optimization of expression conditions; Small, mini- or large-scale expression and affinity purification; Purity evaluation and determination of concentration; Lyophilization of protein.

Phase Service Timeline Phase 1: Subcloning

1 Week

Phase 2:

Small-scale Protein Expression

1 week

Phase 3: Large-scale Protein Expression

1 week

Phase 4:

1.3. Peptide Modification Modification (such as Acetylation, Succinylation and etc.) Special Amino acid (such as Phosphorylation, N-Methyl amino acid and etc.) Labeling (such as Biotinylation, FITC and etc.) Cyclic Peptide (such as Disulfide bridge, amide cyclic and etc.) Peptide Conjugation (such as KLH, BSA and etc.) Quenched fluorescent peptide Multiple Antigenic Peptide

1.4. Peptide Array Peptide array involves thousands of peptides with specified amino acid sequences of various lengths (15-30 mer). The array can be used to study epitope mapping and to identify and optimize leads for a wide range of targets. Contrary to peptide mixtures, the amino acid sequence of each peptide is immediately known without subsequent purification or sequencing. The array, in standard plates or attached to solid supports, can be screened with high throughput using any type of bio-assay, including antibodies, enzymes, receptors and cells.

Protein purification on a large scale

1 week

Description ·

Directly use customer’s clone and expression system without any modification

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Subclone your desired cDNA into an expression vector

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Confirm sequence of the final vector

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Screen desired clones

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Grow the clone in 100 mL of medium

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Express protein in 100 mL of medium

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Pellet cells, lyse cell pellet, centrifuge and collect supernate

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Primary identification of protein expression by immunological assay

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Grow the selected clone in 2 L of medium

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Express protein in 2 L of medium

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Pellet cells, lyse cell pellet, centrifuge and collect supernate

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Purify protein from supernates on affinity chromatography column

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Identify the fractions containing desired protein using immunological assay

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Concentrate the pooled fractions

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Determine the protein concentration

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Yield: Up to 100–500 µg of purified protein

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Lyophilize protein to customer’s specifications

Additional services: Biotinylation of purified protein, Proteolytic cleavage and fragment purification, Sitedirected mutagenesis, Antibody purification and Protein assay development and validation.

Do not forget: RayBiotech is the protein array pioneer company. We provide comprehensive protein array products and services: protein arrays, cytokine antibody arrays, phosphorylation antibody arrays, tissue microarrays, assay design, printing, testing services and consultation.

Polyclonal Antibody Monoclonal Antibody Recombinant Antibody Antibody Engineering Antibody Purification and Conjugation Custom antibody production: Comprehensive and very competitive pricing: Generation of polyclonal, monoclonal, recombinant antibodies as well as their purification and conjugation. Higher titer and affinity: Our proprietary technology enables to make higher affinity of antibody (up to 1:106). Unique and package service: Phosphorylation-specific antibody, converting mAb into single-chain variable fragment (scFv), constructing target-specific antibody library, bi-specific or multiple or functional fusion antibody. Antibody characterization: Characterizing antibodies for use in ELISA, Western blot, immunoprecipitation, immunostaining. Antibody function screening: Its focus on using diseaseassociated tissue or multiple tissue arrays, epitope mapping and protein arrays. Antibody-based custom assay development: Integrating custom antibodies into our signature antibody arrays.