Pretest Praktikum Blok 8.docx

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PRAKTIKUM PEWARNAAN GRAM

- Penisilin V

Alpha:

- Amoksilin

1. 3 reagen warna

- Ampisilin

Carbol Gentian Violet 1%, Gram Iodin/Lugol, Water Fuchsin/Safranin 1% PRAKTIKUM MORFOLOGI KOLONI 2. Warna akhir pemeriksaan gram negatif Alpha: Merah 1. Media yang digunakan pada test biokimia 3. Contoh bakteri Gram Positif & Negatif - Gram Negatif Neisseria gonorrhoeae Klebsiella pneumonia Escherichia coli Pseudomonas aeruginosa Salmonella typhi Vibrio cholera

- Media Cair : seperti kaldu daging, TSB (Trypticase Soy Brot), Alkalis Pepton, media gula-gula dll. - Media semi solid : Semi solid agar, Carry – Blair - Media padat : Salmonella Shigella agar, Blood agar, Nutrient agar, Endo agar, Muller Hinton agar.

- Gram Positif 2. Bentuk koloni K. Pneumoniae Staphylococcus aureus Staphylococcus epidermidis

Koloni besar, memiliki kapsul sehingga berbentuk seperti mukoid, berlendir dan terlihat basah

Streptococcus pyogenes

3. Fungsi blood agar

Bacillus antraxis

Mengetahui kemampuan bakteri dalam melisiskan darah

Lactobacillus sp Clostridium tetani

Gamma: 1. Contoh mikroorganisme gamma hemolisis : staphylococcus epidermidis

PRAKTIKUM ZIEHL-NEELSEN 2. Media apo beee , padat cair Alpha: 1. 3 bahan pemeriksaan ZN

3. Pigmen pseudomonas , pyoverdin pyosianin (kmi dk jwab)

Sputum, urine, cairan otak, getah lambung, ulkus, dll

4. Ciri khas koloni

2. Ditemukan 1-10 BTA dalam 1 lp, minimal 50lp berdasarkan UIATLD ++ (2+) 3. Obat anti TB Rifampisin, Isoniazid, pirazinamid, etambutol, streptomisin, dll.

PRAKTIKUM SENSITIFITAS ANTIBIOTIK (AST) Alpha: 1. 3 cara uji kepekaan - dilusi - Difusi cakram - Difusi dan dilusi 2. Metode yang digunakan dalam uji difusi Metode Kirby-Bauer 3. Sebutkan golongan penisilin minimal 1 - Penisilin G

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