Abraham's Handout Week 7

BICD 120 Fall 2008 Section: Wednesday 6-6:50PM, York 3000A OH: by appointment Handout week 7 Lecture 12 • Auxin receptor

TA: Abraham Tang [email protected] http://plantbio08.wordpress.com

o o Cloned mutated genes  Ubiquitin-mediated proteolysis 

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Psalm 103:15 (NKJV) As for man, his days are like grass; As a flower of the field, so he flourishes.

BICD 120 Fall 2008 Section: Wednesday 6-6:50PM, York 3000A OH: by appointment o

TA: Abraham Tang [email protected] http://plantbio08.wordpress.com

o TIR1-axr  SCF protein binds AUX proteins 

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If you add auxin, ARF becomes free If have SCF mutant… ARF will NOT be released, KEY POINT Mutant situation, aux never binds to SCF-Tir1 Wildtype: binds in presence of auxin TIR1 refers to a wildtype gene that acts as a SCF complex component that normally degrades AUX proteins in presence of auxin (wildtype TIR1 protein) Mutant = tir1-, the SCFTIR1 is inactive and can no longer bind to the aux protein • If you can’t bind aux, you can’t respond to auxin Left side, wildtype, right side mutant • Mutant is auxin RESISTANT

Psalm 103:15 (NKJV) As for man, his days are like grass; As a flower of the field, so he flourishes.

BICD 120 Fall 2008 TA: Abraham Tang Section: Wednesday 6-6:50PM, York 3000A [email protected] OH: by appointment http://plantbio08.wordpress.com • Cyokinin: (+) promotes growth and cell division

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 o Gibberellin (GA) (+) •

Psalm 103:15 (NKJV) As for man, his days are like grass; As a flower of the field, so he flourishes.

BICD 120 Fall 2008 Section: Wednesday 6-6:50PM, York 3000A OH: by appointment • Reproductive growth 

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TA: Abraham Tang [email protected] http://plantbio08.wordpress.com

o 2 types of mutants  Biosynthesis mutant  Receptor mutant ABA- abscisic acid (-)  keeps seed dormant by blocking GA responses 

Psalm 103:15 (NKJV) As for man, his days are like grass; As a flower of the field, so he flourishes.