2009 Abraham Handout Week 9-10

BICD 120 Fall 2009 Section: Wednesday 2-2:50PM, HSS 2321 OH: by appointment

TA: Abraham Tang [email protected] http://plantbuddy.wordpress.com

Dr. Crawford’s Lecture 11/19 1. Be able to draw the T-DNA in detail. Note the order:

a. LB, tms2, tms1, tmr1, opine biosynthesis, RB 2. Pneumonic: Race Cars in South America

a. tmr leads to ________ production b. tms leads to _________ production 3. Tumors a. Tmr+ tms- = ______ tumor b. Tmr- tms+ = ______ tumor c. Tmr+ tms+ = __________ 4. What types of genes are necessary for tumor formation in plants? a. Don’t forget the genes needed for mobilizing T-DNA

5. How can we modify T-DNA to prevent tumor formation and also to select for cells that contain only the transformed DNA? 6. Be able to list out the steps needed to transform plants in a laboratory setting.

7. Be able to describe the binary system and the steps needed to transfer DNA from the agrobacteria T-DNA plasmid into the plant genome. Dr. Crawford’s Lecture 11/24 1. What is needed in order to express different genes into different plants? 2. What is the universal plant promoter? 3. Who is the man given credit for the Green Revolution? 4. Why was the Green Revolution needed? 5. What were the four main components of the Green Revolution that increased crop production? 6. What are the major environmental concerns with industrial agriculture? 7. How can plant genetic engineering can solve industrial agriculture problems? 8. Describe organic farming methods and how they may reduce problems with industrial agriculture. 9. Describe Salatin's methods and why he is able to sustain 400 cow-days/ acre, whereas most average farmers can only sustain 70 cow-days/acre Dr. Crawford’s Lectures Week 10 1. List 3 alternative crop species and the commercial products that may be

derived. 2. Describe the StarLink corn event, including what transgene was in the corn,

why the transgene was problematic, and who was involved in exposing the problem. Mark 4:20 (NKJV) But these are the ones sown on good ground, those who hear the word, accept it, and bear fruit: some thirtyfold, some sixty, and some a hundred.”

BICD 120 Fall 2009 Section: Wednesday 2-2:50PM, HSS 2321 OH: by appointment

TA: Abraham Tang [email protected] http://plantbuddy.wordpress.com

3. Would using new technology to make transgenic plants help to prevent the problems that may arise from transgenic plants like StarLink corn?

Mark 4:20 (NKJV) But these are the ones sown on good ground, those who hear the word, accept it, and bear fruit: some thirtyfold, some sixty, and some a hundred.”