Food borne pathogen –Giardia Karishma Gupte V/05/0246 Fourth year.
Proto zoological deta
ils:
• Enteric protozoa. • Ubiquitously present. • Order –Diplomonatida, Family – Hexamitidae, Genus –Giardia. • Morphology : Dorsal surface – convex; Ventral surface –concave. • Life cycle: Cyst and trophozoite. • G.intestinalis, G.canis, G.cati, G.muris, G. bovis, G.chinchilla.
• Infective dose: 10 to 100 cysts. • Zooanthraponosis. • Raw vegetables, uncooked meat, ready -to- eat foods. • Food borne outbreak. • Water borne outbreak.
Me at indust ry : • Difficult for Meat Inspector. • No pathognomonic lesions. • AMI Sampling Stool samples Microscopy PCR and Genotyping. • Genotype A • HACCP and Microscopy – overestimate the risk of Giardia. • Practicalities ? Carcass rejection Economic loss.
Prev ention & c ontrol: • • • • • • • •
Hygienic practices in food industries. Human waste management. HACCP analysis. Filtration & addition of iodine in water. Heating : 71.5°C for 15 min. Freezing : - 18°C for 1 hour. Phenol. Disease surveillence & epidemiological techniques. • Health education –sanitary conditions and personal cleanliness.
Refer ences: • • • • • •
Zoonoses –Martin E. Hugh. Zoonoses –Mahendra Pal. Protozoology –R.P. Hall. Protozoology –Soulsby. Food borne pathogens –A.H.Varnam. International Life Science Institute (Europe) –Report series. • Internet.