Iso Grid Total Bacterial
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Food Safety Online Ordering
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ For Total Bacterial Count
Disposable NEO-GRID System
Durable ISO-GRID System
Results
Intended Use
Product Specifications
Using Tryptic Soy Fast Green Agar, the ISO-GRID/NEO-GRID Membrane Filter Systems are used to provide a method for determining a sample’s total bacterial count.
Approvals:
The Test The ISO-GRID/NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. A sample’s total bacterial count is enumerated through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on Tryptic Soy Fast Green Agar. Tryptic Soy Fast Green Agar is specifically formulated to provide a total bacterial count. After incubation the membrane filter is examined, and all squares containing one or more green or blue colonies are counted. The total number of positive squares is converted to the corresponding most probable number (MPN) using one of the methods described in the Methods Manual, and the total bacterial count MPN per gram is calculated. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate. Filter 1 mL of the homogenate through the prefilter and ISO-GRID or NEO-GRID membrane filter. Place the membrane filter on the surface of Tryptic Soy Fast Green Agar plate. Incubate inverted for 48 hours at 35–37°C.
Refer to the Methods Manual for complete instructions, and other available test methods.
58
AOAC Official Method #986.32
Tryptic Soy Fast Green Agar Tryptone Soytone Sodium Chloride Fast Green FCF Agar Final pH: 7.3 ± 0.2
15 g/L 5 g/L 5 g/L 0.25 g/L 15 g/L
See Equipment and Accessories - ISO-GRID / NEO-GRID, pg. 95
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6905A TSAF Agar 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ For Total Bacterial Count
Disposable NEO-GRID System
Durable ISO-GRID System
Results
Intended Use
Product Specifications
Using Tryptic Soy Fast Green Agar, the ISO-GRID/NEO-GRID Membrane Filter Systems are used to provide a method for determining a sample’s total bacterial count.
Approvals:
The Test The ISO-GRID/NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. A sample’s total bacterial count is enumerated through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on Tryptic Soy Fast Green Agar. Tryptic Soy Fast Green Agar is specifically formulated to provide a total bacterial count. After incubation the membrane filter is examined, and all squares containing one or more green or blue colonies are counted. The total number of positive squares is converted to the corresponding most probable number (MPN) using one of the methods described in the Methods Manual, and the total bacterial count MPN per gram is calculated. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate. Filter 1 mL of the homogenate through the prefilter and ISO-GRID or NEO-GRID membrane filter. Place the membrane filter on the surface of Tryptic Soy Fast Green Agar plate. Incubate inverted for 48 hours at 35–37°C.
Refer to the Methods Manual for complete instructions, and other available test methods.
58
AOAC Official Method #986.32
Tryptic Soy Fast Green Agar Tryptone Soytone Sodium Chloride Fast Green FCF Agar Final pH: 7.3 ± 0.2
15 g/L 5 g/L 5 g/L 0.25 g/L 15 g/L
See Equipment and Accessories - ISO-GRID / NEO-GRID, pg. 95
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6905A TSAF Agar 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
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