Iso Grid Directcoliform
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Food Safety Online Ordering
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ Direct Coliform/E. coli Enumeration Durable ISO-GRID System
Disposable NEO-GRID System
Results
Coliforms
Intended Use Using Lactose Monensin Glucuronate (LMG) Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for detecting and confirming a sample’s coliform and E. coli count. Buffered MUG Agar (BMA) is used for E. coli confirmation. The Test The ISO-GRID/NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. Coliforms and E. coli are detected through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on LMG Agar. LMG Agar is specifically formulated to detect coliforms. After incubation, the membrane filter is examined for the presence of blue colonies. Any blue (presumptive coliform) colonies present are confirmed using procedures detailed in the Methods Manual. To detect E. coli, transfer the membrane filter to BMA and incubate. E. coli colonies will fluoresce under long wave ultraviolet light. Test Procedure 1. 2. 3. 4. 5. 6. 7.
Prepare a sample homogenate. Filter 1.0 mL of homogenate through an ISO-GRID or NEO-GRID hydrophobic grid membrane filter. Place the membrane filter on Lactose Monensin Glucuronate (LMG) Agar. Incubate for 24 ± 2 hours at 35–37°C. Obtain coliform count. Transfer membrane filter to BMA. Incubate BMA plates for 2 hours at 35–37°C. Examine filter under long wave ultraviolet light. E. coli colonies flouresce bright blue-white on BMA.
Refer to the Methods Manual for complete instructions, and other available test methods. Product Specifications Approvals:
56
AOAC Official Method #990.11
E. coli under long wave ultraviolet light
LMG Agar Tryptose Proteose Peptone SM27 Yeast Extract Lactose Monensin T7 Aniline Blue (CI #42755) Glucuronic Acid, sodium salt Agar Final pH: 7.2 ± 0.2
10 g/L 5 g/L 3 g/L 12.5 g/L 0.038 g/L 0.15 g/L 0.1 g/L 0.5 g/L 15 g/L
Buffered MUG Agar (BMA) Dibasic Sodium Phosphate Monobasic Sodium Phosphate Sodium Chloride 4-Methylumbelliferyl-B-D-glucuronide Agar Final pH: 7.2 ± 0.2
8.23 g/L 1.20 g/L 5 g/L 0.1 g/L (MUG) 15 g/L
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6902A LMG Agar 6903A BMA Agar 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ Direct Coliform/E. coli Enumeration Durable ISO-GRID System
Disposable NEO-GRID System
Results
Coliforms
Intended Use Using Lactose Monensin Glucuronate (LMG) Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for detecting and confirming a sample’s coliform and E. coli count. Buffered MUG Agar (BMA) is used for E. coli confirmation. The Test The ISO-GRID/NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. Coliforms and E. coli are detected through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on LMG Agar. LMG Agar is specifically formulated to detect coliforms. After incubation, the membrane filter is examined for the presence of blue colonies. Any blue (presumptive coliform) colonies present are confirmed using procedures detailed in the Methods Manual. To detect E. coli, transfer the membrane filter to BMA and incubate. E. coli colonies will fluoresce under long wave ultraviolet light. Test Procedure 1. 2. 3. 4. 5. 6. 7.
Prepare a sample homogenate. Filter 1.0 mL of homogenate through an ISO-GRID or NEO-GRID hydrophobic grid membrane filter. Place the membrane filter on Lactose Monensin Glucuronate (LMG) Agar. Incubate for 24 ± 2 hours at 35–37°C. Obtain coliform count. Transfer membrane filter to BMA. Incubate BMA plates for 2 hours at 35–37°C. Examine filter under long wave ultraviolet light. E. coli colonies flouresce bright blue-white on BMA.
Refer to the Methods Manual for complete instructions, and other available test methods. Product Specifications Approvals:
56
AOAC Official Method #990.11
E. coli under long wave ultraviolet light
LMG Agar Tryptose Proteose Peptone SM27 Yeast Extract Lactose Monensin T7 Aniline Blue (CI #42755) Glucuronic Acid, sodium salt Agar Final pH: 7.2 ± 0.2
10 g/L 5 g/L 3 g/L 12.5 g/L 0.038 g/L 0.15 g/L 0.1 g/L 0.5 g/L 15 g/L
Buffered MUG Agar (BMA) Dibasic Sodium Phosphate Monobasic Sodium Phosphate Sodium Chloride 4-Methylumbelliferyl-B-D-glucuronide Agar Final pH: 7.2 ± 0.2
8.23 g/L 1.20 g/L 5 g/L 0.1 g/L (MUG) 15 g/L
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6902A LMG Agar 6903A BMA Agar 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
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