Iso Grid Listeria
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Food Safety Online Ordering
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ 24-Hour Presumptive Enumeration of Listeria spp. and L. monocytogenes in Foods
Disposable NEO-GRID System
Durable ISO-GRID System
Results
Using LM-137 Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for presumptively enumerating Listeria spp. and Listeria monocytogenes in foods in 24 hours.
D-Glucose Sodium Carbonate Acriflavin Liver Powder Agar Final pH: 7.4 - 7.5
The Test
Presterilized Supplements
Intended Use
The ISO-GRID and NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. Listeria spp. and Listeria monocytogenes are detected through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on LM-137 Agar. LM-137 Agar is specifically formulated to detect Listeria spp. and Listeria monocytogenes. After incubation, the membrane filter is examined for the presence of homogeneous pink, flat, small to medium-sized colonies. Any colonies of this type are a presumptive positive for Listeria spp. and/or Listeria monocytogenes. These presumptive colonies must be confirmed using procedures detailed in the Methods Manual. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate. Filter 1 mL of homogenate through an ISO-GRID or NEO-GRID hydrophobic grid membrane filter. Place the membrane filter on LM-137 Agar. Incubate for 22–24 hours at 35 - 37°C.
Refer to the Methods Manual for complete instructions, and other available test methods. LM-137 Agar Tryptone Yeast Extract Sodium Pyruvate Lithium Chloride MgSO4•7H2O
10 g/L 1 g/L 10 g/L 5 g/L 7.4 g/L
1 g/L 1 g/L 0.015 g/L 10 g/L 15 g/L
• Egg Yolk Emulsion (50%) - available from Neogen. DO NOT USE PRODUCT CONTAINING TELLURITE. • LM-137 Emulsion (included with LM-137 Agar).
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6913A LM-137 Agar + 4 Supplements 7982 Egg Yolk Emulsion (50%), 100 mL 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
45
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ 24-Hour Presumptive Enumeration of Listeria spp. and L. monocytogenes in Foods
Disposable NEO-GRID System
Durable ISO-GRID System
Results
Using LM-137 Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for presumptively enumerating Listeria spp. and Listeria monocytogenes in foods in 24 hours.
D-Glucose Sodium Carbonate Acriflavin Liver Powder Agar Final pH: 7.4 - 7.5
The Test
Presterilized Supplements
Intended Use
The ISO-GRID and NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. Listeria spp. and Listeria monocytogenes are detected through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on LM-137 Agar. LM-137 Agar is specifically formulated to detect Listeria spp. and Listeria monocytogenes. After incubation, the membrane filter is examined for the presence of homogeneous pink, flat, small to medium-sized colonies. Any colonies of this type are a presumptive positive for Listeria spp. and/or Listeria monocytogenes. These presumptive colonies must be confirmed using procedures detailed in the Methods Manual. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate. Filter 1 mL of homogenate through an ISO-GRID or NEO-GRID hydrophobic grid membrane filter. Place the membrane filter on LM-137 Agar. Incubate for 22–24 hours at 35 - 37°C.
Refer to the Methods Manual for complete instructions, and other available test methods. LM-137 Agar Tryptone Yeast Extract Sodium Pyruvate Lithium Chloride MgSO4•7H2O
10 g/L 1 g/L 10 g/L 5 g/L 7.4 g/L
1 g/L 1 g/L 0.015 g/L 10 g/L 15 g/L
• Egg Yolk Emulsion (50%) - available from Neogen. DO NOT USE PRODUCT CONTAINING TELLURITE. • LM-137 Emulsion (included with LM-137 Agar).
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6913A LM-137 Agar + 4 Supplements 7982 Egg Yolk Emulsion (50%), 100 mL 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
45
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