Iso Grid Staph
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Food Safety Online Ordering
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ For Enumeration of Staphylococcus aureus in Foods
Disposable NEO-GRID System
Durable ISO-GRID System
Results
Intended Use
Baird-Parker Egg Yolk Agar
Using Baird-Parker Egg Yolk Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for enumerating Staphylococcus aureus in foods.
Baird-Parker Agar Tryptone Beef Extract Yeast Extract Sodium Pyruvate Glycine Lithium Chloride-6-Hydrate Agar Final pH: 7.0 ± 0.2 @ 25°C
The Test The ISO-GRID/NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration (HGMF). Staphylococcus aureus is detected through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic grid membrane filter, and the membrane is placed on Baird-Parker Egg Yolk Agar. Baird-Parker Egg Yolk Agar is specifically formulated to detect Staphylococcus aureus. After incubation the membrane filter is examined for the presence of black or dark grey colonies. Any black or dark gray colonies are a presumptive positive for Staphylococcus aureus, and should be confirmed using procedures detailed in the Methods Manual. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate. Filter 1 mL of homogenate through an ISO-GRID or NEO-GRID hydrophobic grid membrane filter. Place the membrane filter on Baird-Parker Egg Yolk Agar. Incubate for 48 hours at 35 - 37°C.
Refer to the Methods Manual for complete instructions, and other available test methods.
54
Egg Yolk Tellurite Emulsion (100 mL) Chicken egg yolk Potassium Tellurite
10 g/L 5 g/L 1 g/L 10 g/L 12 g/L 5 g/L 17 g/L
100% solution 0.21 g
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 7112 Baird-Parker Agar 7983 Egg Yolk Tellurite Emulsion (100 mL) 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ For Enumeration of Staphylococcus aureus in Foods
Disposable NEO-GRID System
Durable ISO-GRID System
Results
Intended Use
Baird-Parker Egg Yolk Agar
Using Baird-Parker Egg Yolk Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for enumerating Staphylococcus aureus in foods.
Baird-Parker Agar Tryptone Beef Extract Yeast Extract Sodium Pyruvate Glycine Lithium Chloride-6-Hydrate Agar Final pH: 7.0 ± 0.2 @ 25°C
The Test The ISO-GRID/NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration (HGMF). Staphylococcus aureus is detected through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic grid membrane filter, and the membrane is placed on Baird-Parker Egg Yolk Agar. Baird-Parker Egg Yolk Agar is specifically formulated to detect Staphylococcus aureus. After incubation the membrane filter is examined for the presence of black or dark grey colonies. Any black or dark gray colonies are a presumptive positive for Staphylococcus aureus, and should be confirmed using procedures detailed in the Methods Manual. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate. Filter 1 mL of homogenate through an ISO-GRID or NEO-GRID hydrophobic grid membrane filter. Place the membrane filter on Baird-Parker Egg Yolk Agar. Incubate for 48 hours at 35 - 37°C.
Refer to the Methods Manual for complete instructions, and other available test methods.
54
Egg Yolk Tellurite Emulsion (100 mL) Chicken egg yolk Potassium Tellurite
10 g/L 5 g/L 1 g/L 10 g/L 12 g/L 5 g/L 17 g/L
100% solution 0.21 g
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 7112 Baird-Parker Agar 7983 Egg Yolk Tellurite Emulsion (100 mL) 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
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