Enhanced Inhibitory Effects Of Hyperthermia Combined With Ascorbic Acid On Dna Synthesis In Ehrlich Ascites Tumor Cells Grown At A Low Cell Density

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Titre du document / Document title Enhanced inhibitory effects of hyperthermia combined with ascorbic acid on DNA synthesis in Ehrlich ascites tumor cells grown at a low cell density Auteur(s) / Author(s) KAGEYAMA K. (1) ; ONOYAMA Y. ; OTANI S. ; MATSUI-YUASA I. ; NAGAO N. ; MIWA N. ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s) (1) Osaka city univ. medical school, radioisotope cent., Osaka 545, JAPON Résumé / Abstract Effects of hyperthermia and cell densities on inhibitory activity of ascorbic acid on DNA synthesis in Ehrlich ascites tumor cells were studied. When cells at a low density of 5 x 103/ml were treated with 75 μ M ascorbic acid for 1 h, DNA synthesis was inhibited after treatment at 37°C and the inhibition was significantly enhanced at 42°C. At a cell density as high as 1 x 105/ml, however, inhibition did not occur at 37°C or 42°C. In contrast, dehydroascorbic acid was inactive even at a low cell density under similar conditions. Inhibitory effects of ascorbic acid on DNA synthesis were also markedly enhanced by treatment at 40°C. DNA synthesis was not inhibited in the absence of the drug. Futhermore, mice transplanted with cells treated with a combination of 75 μ M ascorbic acid and hyperthermia at 42°C, considerably prolonged their survival time in comparison with untreated cells. Addition of ascorbic acid to hyperthermia is suggested to be an advantageous treatment for cancer Revue / Journal Title Cancer biochemistry biophysics ISSN 0305-7232 CODEN CABCD4 Source / Source 1995, vol. 14, no4, pp. 273-280 (22 ref.) Langue / Language Anglais Editeur / Publisher Gordon and Breach, London, ROYAUME-UNI (1974-1999) (Revue) Mots-clés anglais / English Keywords Ehrlich ascites tumor ; Ascites ; Animal ; Ascorbic acid ; Chemotherapy ; Combined treatment ; Induced hyperthermia ; In vitro ; In vivo ; Cell culture ; Cell density ; Biological activity ; DNA ; Biosynthesis ; Cell proliferation ; Spontaneous ; Mouse ; Vitamin ; Rodentia ; Mammalia ; Vertebrata ; Mammary gland diseases ; Malignant tumor ; Adenocarcinoma ; Mots-clés français / French Keywords Tumeur Ehrlich ; Ascite ; Animal ; Ascorbique acide ; Chimiothérapie ; Traitement associé ; Hyperthermie provoquée ; In vitro ; In vivo ; Culture cellulaire ; Densité cellulaire ; Activité biologique ; DNA ; Biosynthèse ; Multiplication cellulaire ; Spontané ; Souris ; Vitamine ; Rodentia ; Mammalia ; Vertebrata ; Glande mammaire pathologie ; Tumeur maligne ; Epithélioma glandulaire ; Mots-clés espagnols / Spanish Keywords Tumor Ehrlich ; Ascitis ; Animal ; Ascórbico ácido ; Quimioterapia ; Tratamiento asociado ; Hipertermia provocada ; In vitro ; In vivo ; Cultivo celular ; Densidad celular ; Actividad biológica ; DNA ; Biosíntesis ; Multiplicación celular ; Espontáneo ; Ratón ; Vitamina ; Rodentia ; Mammalia ; Vertebrata ; Glándula mamaria patología ; Tumor maligno ; Adenocarcinoma ;

Localisation / Location INIST-CNRS, Cote INIST : 16794, 35400005933339.0060 Nº notice refdoc (ud4) : 3471870

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