Cinetica Enzimatiza

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Sustrato Concentración (M) 0.3 X 10-3 0.5 X 10-3 1.0 X 10-3 3.0 X 10-3 9.0 X 10-3

Inverso (1/M)

Sustrato Concentración (M) 0 0 0 0 0.01 0

0.1400 0.1350 0.1300 0.1250 0.1200

SO DE VELOCIDAD

0.1150 0.1100 0.1050 0.1000 0.0950 0.0900 0.0850 0.0800 0.0750 0.0700 0.0650 0.0600 0.0550 0.0500

Inverso (1/M) 3333.33 2000.00 1000.00 333.33 111.11

INVERSO DE

0.0750 0.0700 0.0650 0.0600 0.0550 0.0500 0.0450 0.0400 0.0350 0.0300 0.0250 0.0200 0.0150 0.0100 0.0050 0.0000 -4000.00

-3000.00

KM SIN INIHIBIDOR KM CON INHIBIDOR

TIPO DE INHIBICIO:

-2000.00

Velocidad (µmoles/min)

Sin inhibidor

Con Inhibidor

10.4 14.5 22.5 33.8 40.5

8.2 12.12 19.05 32.26 40 Velocidad (µmoles/min)

Sin inhibidor

Con Inhibidor

10.4 14.5 22.5 33.8 40.5 10.4

8.2 12.12 19.05 32.26 40 8.2

-2000.00

-1000.00

0.00

1000.00

INVERSOS DE SUSTRATO 0M 0M

COMPETITIVA.

Inversos de la velocidad (min/µmoles)

Sin inhibidor

Con Inhibidor

Inversos de la velocidad (min/µmoles)

=

Sin inhibidor

Con Inhibidor

0.0962 0.0690 0.0444 0.0296 0.0247

0.1220 0.0825 0.0525 0.0310 0.0250

1000.00

2000.00

3000.00

400

SUSTRATO VMAX CON O SIN INHIBIDOR:

50

Column E Column F

0

Column E Column F

4000.00

(µmoles/min)

-3 -2 -1 0 1 2 3

-3 -2 -1 0 1 2 3

3 2 1 0 -1 -2 -3 Row 1

Row 2

Row 3

1

Column A

Row 2

Row 3

Row 4

Row 5

Row 6

Row 7

Velocidad (µmoles/min)

Sustrato Concentración (M) 0.3 X 10-5 0.5 X 10-5 1.0 X 10-5 3.0 X 10-5 9.0 X 10-5

Inverso (1/M)

14.5 22.5 33.8 40.5

Velocidad (µmoles/min)

Sustrato Concentración (M) 0 0 0 0 0

Sin inhibidor 10.4

Inverso (1/M) 333333.33 200000 100000 33333.33 11111.11 -333333.33

0.12 0.12 0.12 0.12 0.11 0.11 0.11 0.11 0.1 0.1 0.1 0.1 0.09 0.09 0.09 0.09 0.08 0.08 0.08

Sin inhibidor 10.4

14.5 22.5 33.8 40.5

0.09 0.09 0.09 0.09 0.08 0.08 0.08 0.08 0.07 0.07 0.07 0.07 0.06 0.06 0.06 0.06 0.05 0.05 0.05 0.05 0.04 0.04 0.04 0.04 0.03 0.03

50000 25000

125000

0.03 0.03 0.02 -350000

-300000

-250000

75000

KM SIN INIHIBIDOR KM CON INHIBIDOR

TIPO DE INHIBICIO:

-20000

locidad (µmoles/min) Con Inhibidor

Inversos de la velocidad (min/µmoles) Sin inhibidor

Con Inhibidor

8.2 12.12 19.05 32.26 40

locidad (µmoles/min)

Inversos de la velocidad (min/µmoles)

Con Inhibidor

Sin inhibidor

Con Inhibidor

8.2 12.12 19.05 32.26 40

0.1 0.07 0.04 0.03 0.02

0.12 0.08 0.05 0.03 0.03

300000

-250000

-200000

0M 0.0000133333 M

COMPETITIVA.

-150000

-100000

-50000

VMAX CON O SIN INHIBIDOR: 40

0

50000

1

0

0

50000

(µmoles/min)

100000

150000

200000

250000

300000

35000

00

Column E Column F

300000

350000

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