Brom Add Results Collated
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Bromalain addition technique AHG card and standard AHG technique Anti-D 73/515 R1R1 cell batch R1023272 Expire 19/2/09 test date 20/1/09 Bromalain additon AHG card Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 2 1 0 0 0 Exp2 4 3 1 0 0 0 Exp3 4 2 1 0 0 0 Standard AHG technique Dilution 1:100 1:400 Exp 1 4 3 Exp2 4 3 Exp3 4 3
1:800 2 2 2
1:1600 1:3200 1:6400 1 0.5 0 1 0.5 0 1 0.5 0
Anti-D 86 i.u. R1R1 cell batch R1023272 Expire 19/2/09 test date 20/1/09 Bromalain additon AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 1 0 0 0 0 Exp2 4 1 0 0 0 0 Exp3 4 1 0 0 0 0 Standard AHG technique Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 3 0.5 ? 0 0 Exp2 4 3 2 ? 0 0 Exp3 4 3 1 ? 0 0
Anti-D Lot 24311 R1R1 cell batch R1023272 Expire 19/2/09 test date 20/1/09 Bromalain additon AHG card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Standard AHG technique Diltuion 1:4 1:8 Exp 1 2 2 Exp2 3 2 Exp3 2 2
1:16 1 1 1
1:32 0 0 0
1:64 0 0 0
1:128 0 0 0
Anti-D Lot 32372B R1R1 cell batch R1023272 Expire 19/2/09 test date 20/1/09 Bromalain additon AHG card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 Exp2 Exp3
4 4 3
3 3 2
Standard AHG technique Dilution 1:200 1:400 Exp 1 3 2 Exp2 3 2 Exp3 3 2
1 1 1
1:800 1 1 1
0 0 0
0 0 0
0 0 0
1:1600 1:3200 1:6400 ? 0 0 0.5 0 0 0.5 0 0
Anti-D 73/515 R1R1 cell batch 45161.85.X Expire 2/2/09 test date 20/1/09 Standard AHG technique Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0
Anti-D 86i.u. R1R1 cell batch 45161.85.X Expire 2/2/09 test date 20/1/09 Standard AHG technique Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 2 0.5 0 0 0 Exp2 4 2 1 0 0 0 Exp3 4 2 0.5 0 0 0
Anti-D 73/515 Titre R2R2 cell Bromelain addition technique Batch R1023272 Expire 19/2/09 Test date AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4 Saline card Dilution 1:100 Exp 1 4 Exp 2 4 Exp3 4
1:400 3 3 2
1:800 2 2 1
1:1600 1:3200 1:6400 0 0 0 ? 0 0 0 0 0 Test date 19/1/09
1:400 3 3 2
1:800 1 0.5 0.5
1:1600 1:3200 1:6400 0 0 0 0 0 0 0 0 0 Test date 19/1/09
Anti-D 86i.u. Titre R2R2 cell Bromelain addition technique Batch R1023272 Expire 19/2/09 Test date 1 AHG card Dilution 1:400 Exp 1 4 Exp 2 4 Exp 3 4 Saline card Dilution 1:400 Exp 1 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 3 2 0.5 0 0 3 2 0.5 0 0 3 2 ? 0 0 Test date 19/1/09 1:1600 1:3200 1:6400 1:12800 1:25600 2 0.5 0 0 0 2 0.5 0 0 0 3 0 0 0 0 Test date 19/1/09
Anti-D Lot 24311 Titre R2R2 cell Bromelain addition technique Batch R1023272 Expire 19/2/09 Test d AHG card Diltuion 1:4 Exp 1 3 Exp 2 3 Exp 3 4 Saline card Diltuion 1:4 Exp 1 3 Exp 2 3 Exp 3 4
1:8 3 3 3
1:16 2 2 2
1:32 2 2 1
1:64 ? ? 0
1:128 0 0 0 Test date 19/1/09
1:8 3 3 3
1:16 ? ? 2
1:32 0 0 ?
1:64 0 0 0
1:128 0 0 0 Test date 19/1/09
2 Expire 19/2/09 Test date 15/1/09
Expire 19/2/09 Test date 15/1/09
3272 Expire 19/2/09 Test date 15/1/09
Bromelain addition technique Pooled R1R1 cells Pool A Anti-D 73/515 AHG card Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 0 0 0 Exp2 4 3 2 0 0 0 Exp3 4 3 2 0 0 0 Anti-D 86 i.u. AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 1 0 0 0 0 Exp2 4 1 0 0 0 0 Exp3 4 1 0 0 0 0 Anti-D lot 24311 AHG card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 32372B AHG card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 101 AHG card Dilution 1:50 1:100 1:200 1:400 1:800 1:1600 Exp 1 3 2 0 0 0 0 Exp2 4 2 0 0 0 0 Exp3 4 2 0 0 0 0 Anti-D Patient Pool A AHG card Dilution Neat 1:2 1:4 1:8 1:16 1:32 Exp 1 2 1 0 0 0 0 Exp2 2 1 0 0 0 0 Exp3 2 1 0 0 0 0 Anti-D Lot 102 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 3 2 1 0 0 0 Exp2 3 2 1 0 0 0 Exp3 3 2 1 0 0 0 Anti-D Lot 201 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 2 Exp2 2 Exp3 2 Anti-D Lot 202 AHG card Dilution 1:100 Exp 1 1 Exp2 1 Exp3 1 Anti-D Lot 103 AHG card Dilution 1:100 Exp 1 4 Exp2 4 Exp3 4 Anti-D Lot 203 AHG card Dilution 1:200 Exp 1 4 Exp2 4 Exp3 4
1 1 1
0 0 0
0 0 0
0 0 0
0 0 0
1:200 0 0 0
1:400 0 0 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
1:200 3 3 3
1:400 3 3 3
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
1:400 4 4 4
1:800 3 3 3
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
1:800 2 2 2
1:1600 1:3200 1:6400 1 0.5 0 1 0.5 0 1 0.5 0
Anti-D 86 i.u. R1R1 cell batch R1023272 Expire 19/2/09 test date 20/1/09 Bromalain additon AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 1 0 0 0 0 Exp2 4 1 0 0 0 0 Exp3 4 1 0 0 0 0 Standard AHG technique Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 3 0.5 ? 0 0 Exp2 4 3 2 ? 0 0 Exp3 4 3 1 ? 0 0
Anti-D Lot 24311 R1R1 cell batch R1023272 Expire 19/2/09 test date 20/1/09 Bromalain additon AHG card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Standard AHG technique Diltuion 1:4 1:8 Exp 1 2 2 Exp2 3 2 Exp3 2 2
1:16 1 1 1
1:32 0 0 0
1:64 0 0 0
1:128 0 0 0
Anti-D Lot 32372B R1R1 cell batch R1023272 Expire 19/2/09 test date 20/1/09 Bromalain additon AHG card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 Exp2 Exp3
4 4 3
3 3 2
Standard AHG technique Dilution 1:200 1:400 Exp 1 3 2 Exp2 3 2 Exp3 3 2
1 1 1
1:800 1 1 1
0 0 0
0 0 0
0 0 0
1:1600 1:3200 1:6400 ? 0 0 0.5 0 0 0.5 0 0
Anti-D 73/515 R1R1 cell batch 45161.85.X Expire 2/2/09 test date 20/1/09 Standard AHG technique Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0
Anti-D 86i.u. R1R1 cell batch 45161.85.X Expire 2/2/09 test date 20/1/09 Standard AHG technique Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 2 0.5 0 0 0 Exp2 4 2 1 0 0 0 Exp3 4 2 0.5 0 0 0
Anti-D 73/515 Titre R2R2 cell Bromelain addition technique Batch R1023272 Expire 19/2/09 Test date AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4 Saline card Dilution 1:100 Exp 1 4 Exp 2 4 Exp3 4
1:400 3 3 2
1:800 2 2 1
1:1600 1:3200 1:6400 0 0 0 ? 0 0 0 0 0 Test date 19/1/09
1:400 3 3 2
1:800 1 0.5 0.5
1:1600 1:3200 1:6400 0 0 0 0 0 0 0 0 0 Test date 19/1/09
Anti-D 86i.u. Titre R2R2 cell Bromelain addition technique Batch R1023272 Expire 19/2/09 Test date 1 AHG card Dilution 1:400 Exp 1 4 Exp 2 4 Exp 3 4 Saline card Dilution 1:400 Exp 1 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 3 2 0.5 0 0 3 2 0.5 0 0 3 2 ? 0 0 Test date 19/1/09 1:1600 1:3200 1:6400 1:12800 1:25600 2 0.5 0 0 0 2 0.5 0 0 0 3 0 0 0 0 Test date 19/1/09
Anti-D Lot 24311 Titre R2R2 cell Bromelain addition technique Batch R1023272 Expire 19/2/09 Test d AHG card Diltuion 1:4 Exp 1 3 Exp 2 3 Exp 3 4 Saline card Diltuion 1:4 Exp 1 3 Exp 2 3 Exp 3 4
1:8 3 3 3
1:16 2 2 2
1:32 2 2 1
1:64 ? ? 0
1:128 0 0 0 Test date 19/1/09
1:8 3 3 3
1:16 ? ? 2
1:32 0 0 ?
1:64 0 0 0
1:128 0 0 0 Test date 19/1/09
2 Expire 19/2/09 Test date 15/1/09
Expire 19/2/09 Test date 15/1/09
3272 Expire 19/2/09 Test date 15/1/09
Bromelain addition technique Pooled R1R1 cells Pool A Anti-D 73/515 AHG card Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 0 0 0 Exp2 4 3 2 0 0 0 Exp3 4 3 2 0 0 0 Anti-D 86 i.u. AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 1 0 0 0 0 Exp2 4 1 0 0 0 0 Exp3 4 1 0 0 0 0 Anti-D lot 24311 AHG card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 32372B AHG card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 101 AHG card Dilution 1:50 1:100 1:200 1:400 1:800 1:1600 Exp 1 3 2 0 0 0 0 Exp2 4 2 0 0 0 0 Exp3 4 2 0 0 0 0 Anti-D Patient Pool A AHG card Dilution Neat 1:2 1:4 1:8 1:16 1:32 Exp 1 2 1 0 0 0 0 Exp2 2 1 0 0 0 0 Exp3 2 1 0 0 0 0 Anti-D Lot 102 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 3 2 1 0 0 0 Exp2 3 2 1 0 0 0 Exp3 3 2 1 0 0 0 Anti-D Lot 201 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 2 Exp2 2 Exp3 2 Anti-D Lot 202 AHG card Dilution 1:100 Exp 1 1 Exp2 1 Exp3 1 Anti-D Lot 103 AHG card Dilution 1:100 Exp 1 4 Exp2 4 Exp3 4 Anti-D Lot 203 AHG card Dilution 1:200 Exp 1 4 Exp2 4 Exp3 4
1 1 1
0 0 0
0 0 0
0 0 0
0 0 0
1:200 0 0 0
1:400 0 0 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
1:200 3 3 3
1:400 3 3 3
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
1:400 4 4 4
1:800 3 3 3
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
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