Pre Brom Cells 26.1.09
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Pre Bromalain treated R1R1 pooled Treated cells ate date 26/1/09 22/1/09 test Test date date 26/1/09 26/1/09 Anti-D 73/515 Saline card Dilution 1:100 Exp 1 4
1:400 3
1:800 2
1:1600 1:3200 1:6400 1 0 0
AHG card Dilution 1:100 Exp1 4
1:400 4
1:800 2
1:1600 1:3200 1:6400 2 1 0
reated cells washed in cellstab AHG card Dilution 1:100 1:400 Exp1 4 3
1:800 2
1:1600 1:3200 1:6400 1 0 0
Pre bromalain treated pooled rr red treated cells Treated date 26/1/09 26/1/09 tdatetest 26/01/09 Test date date 26/1/09 26/1/09 Anti-D 73/515 Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Sal 0 0 0 0 0 0 AHG 0 0 0 0 0 0 Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D 86 i.u. Saline card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 2 1 0 0 0 Exp 2 4 2 1 0 0 0 Exp 3 4 2 1 0 0 0 AHG card Dilution 1:400 Exp 1 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 2 0 0 4 3 2 0 0 3 3 1 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 3 2 1 0 0 Exp 2 4 3 2 1 0 0 Exp 3 4 3 2 1 0 0 Pre Bromalain treated R1R1 pooled Treated cells ate date 26/1/09 26/1/09 9 Test test Test date date date 27/1/09 27/1/09 Anti-D lot 24311 Saline card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 2 0 0 Exp 3 4 4 3 2 0 0
AHG card Dilution 1:4 Exp 1 4 Exp 2 4 Exp 3 4
1:8 4 4 4
1:16 3 3 3
1:32 2 2 3
1:64 1 1 2
1:128 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:4 1:8 1:16 Exp 1 4 3 2 Exp 2 4 3 2 Exp 3 4 3 2
1:32 1 0 1
1:64 0 0 0
1:128 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D Lot 32372B Saline card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 2 1 0 0 Exp 2 4 4 2 1 0 0 Exp 3 4 4 2 1 0 0 AHG card Dilution 1:200 Exp 1 4 Exp 2 4 Exp 3 4
1:400 4 4 4
1:800 4 3 4
1:1600 1:3200 1:6400 2 1 0 2 1 0 2 1 0
Untreated cells in cellstab Standard AHG technique Dilution 1:200 1:400 1:800 Exp 1 4 3 2 Exp 2 4 3 2 Exp 3 4 3 2
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D Lot 101 Saline card Dilution 1:50 1:100 1:200 1:400 1:800 1:1600 Exp 1 4 2 0 0 0 0 Exp 2 4 2 1 0 0 0 Exp 3 4 2 1 0 0 0 AHG card Dilution 1:50 Exp 1 4 Exp 2 4 Exp 3 4
1:100 4 4 4
1:200 2 2 2
1:400 1 0 1
1:800 0 0 0
1:1600 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:50 1:100 1:200 Exp 1 2 2 1 Exp 2 3 2 0 Exp 3 3 2 0
1:400 0 0 0
1:800 0 0 0
1:1600 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D Patient Pool A Saline card Dilution Neat 1:2 1:4 1:8 1:16 1:32 Exp 1 2 1 0 0 0 0 Exp 2 2 1 0 0 0 0 Exp 3 3 1 0 0 0 0 AHG card Dilution Neat Exp 1 4 Exp 2 4 Exp 3 4
1:2 2 2 2
Untreated cells in cellstab Standard AHG technique Dilution Neat 1:2 Exp 1 2 1 Exp 2 2 1 Exp 3 2 1
1:4 1 1 1
1:8 0 0 0
1:16 0 0 0
1:32 0 0 0
1:4 0 0 0
1:8 0 0 0
1:16 0 0 0
1:32 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 27/1/09 Anti-D Lot 102 Saline card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 4 3 1 0 0 0 Exp 2 4 4 1 0 0 0 Exp 3 4 3 1 0 0 0 AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4
1:200 3 3 3
1:400 2 2 2
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:100 1:200 1:400 Exp 1 3 2 1 Exp 2 3 2 1 Exp 3 3 2 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 27/1/09 Anti-D Lot 201 Saline card Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600 Exp 1 2 0 0 0 0 0 Exp 2 2 0 0 0 0 0 Exp 3 2 0 0 0 0 0 Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 3 0 0 0 0 0 AHG card Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600 Exp 1 3 2 0 0 0 0 Exp 2 3 2 0 0 0 0 Exp 3 3 2 0 0 0 0 Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 4 2 1 0 0 0 Untreated cells in cellstab Standard AHG technique Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600 Exp 1 2 1 0 0 0 0 Exp 2 2 1 0 0 0 0 Exp 3 2 1 0 0 0 0 Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 4 3 1 0 0 0 Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 27/1/09 Anti-D Lot 202 Saline card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 2 0 0 0 0 0 Exp 2 2 0 0 0 0 0 Exp 3 2 0 0 0 0 0 AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4
1:200 2 2 2
1:400 1* 1* 1*
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:100 1:200 1:400 Exp 1 2 1 0 Exp 2 2 1 0 Exp 3 2 1 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
Pre bromalain treated cells R1R1 pool treated date 26/1/09 test date 27/1/09 Anti-D 73/515 Saline card Dilution 1:100 Exp 1 4 Exp 2 4 Exp3 4
1:400 3 3 3
1:800 3 3 3
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp3 4
1:400 4 4 4
1:800 3 3 3
1:1600 1:3200 1:6400 2 1 0 2 1 0 2 1 0
R1R1 cells in cellstab Dilution 1:100 1:400 Exp 1 4 3 Exp 2 4 3 Exp3 4 3
1:800 2 2 2
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test 2009-1-29 date 27/1/09 Anti-D Lot 103 Saline card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 3 3 2 1 0 0 Exp 2 3 3 2 1 0 0 Exp 3 3 2 2 1 0 0 AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4
1:200 3 3 3
1:400 2 2 2
1:800 2 2 1
1:1600 1:3200 1 0 1 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:100 1:200 1:400 Exp 1 4 4 3 Exp 2 4 4 3 Exp 3 4 3 3
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 29/1/09 Anti-D Lot 203 Saline card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 2 1 0
Exp 3
4
4
3
1:400 4 4 4
1:800 4 4 3
1:1600 1:3200 1:6400 3 2 0 2 2 0 2 1 0
Untreated cells in cellstab Standard AHG technique Dilution 1:200 1:400 1:800 Exp 1 4 4 3 Exp 2 4 4 3 Exp 3 4 4 3
1:1600 1:3200 1:6400 2 1 0 2 0 0 2 1 0
AHG card Dilution 1:200 Exp 1 4 Exp 2 4 Exp 3 4
3
1
0
AHG Technique inital titre R1R1 pooled cells Dilution Sample Neat 1:2 1:4 1:8 Lot 101 4 4 4 4 Pat. Pool A 2 1 0 0 Lot 102 4 4 4 4 Lot 201 4 4 4 4 Lot 202 4 4 4 4 Lot 103 4 4 4 4 Lot 203 4 4 4 4
1:16 4 0 4 4 4 4 4
1:32 4 0 4 4 4 4 4
1:64 2 4 4 3 4 4
1:128 1 3 4 2 4 4
1:256 0 2 3 1 3 4
1:512 0 1 2 0 3 4
1:1023 1:2046 0 0 0 1 0 2 3
0 0 0 1 3
Bromelain addition technique Pooled R1R1 cells Pool A Anti-D 73/515 AHG card Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 0 0 0 Exp2 4 3 2 0 0 0 Exp3 4 3 2 0 0 0 Anti-D 86 i.u. AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 1 0 0 0 0 Exp2 4 1 0 0 0 0 Exp3 4 1 0 0 0 0 Anti-D lot 24311 AHG card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 32372B AHG card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 101 AHG card Dilution 1:50 1:100 1:200 1:400 1:800 1:1600 Exp 1 3 2 0 0 0 0 Exp2 4 2 0 0 0 0 Exp3 4 2 0 0 0 0 Anti-D Patient Pool A AHG card Dilution Neat 1:2 1:4 1:8 1:16 1:32 Exp 1 2 1 0 0 0 0 Exp2 2 1 0 0 0 0 Exp3 2 1 0 0 0 0 Anti-D Lot 102 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 3 2 1 0 0 0 Exp2 3 2 1 0 0 0 Exp3 3 2 1 0 0 0 Anti-D Lot 201 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 2 1 0 0 0 0 Exp2 2 1 0 0 0 0 Exp3 2 1 0 0 0 0
Anti-D Lot 202 AHG card Dilution 1:100 Exp 1 1 Exp2 1 Exp3 1 Anti-D Lot 103 AHG card Dilution 1:100 Exp 1 4 Exp2 4 Exp3 4 Anti-D Lot 203 AHG card Dilution 1:200 Exp 1 4 Exp2 4 Exp3 4
1:200 0 0 0
1:400 0 0 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
1:200 3 3 3
1:400 3 3 3
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
1:400 4 4 4
1:800 3 3 3
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
Papain treated R1R1 pooled cells Saline cards Anti-D 73/515 Dilution 1:100 Exp 1 4 Exp 2 4 Anti-D 86 i.u. Dilution 1:400 Exp 1 4 Exp 2 4 Anti-D lot 24311 Dilution 1:4 Exp 1 4 Exp 2 4
1:400 3 3
1:800 2 3
1:1600 1:3200 1:6400 1 1 0 2 1 1
1:1600 1:3200 1:6400 1:12800 1:25600 3 1 0 0 0 3 2 0 0 0
1:8 4 4
1:16 3 3
1:32 2 3
1:64 2 1
1:128 1 0
Anti-D Lot 32372B Dilution 1:200 1:400 Exp 1 4 4 Exp 2 4 4
1:800 3 3
Anti-D Lot 101 Dilution 1:50 Exp 1 4 Exp 2 4
1:200 3 2
1:400 1 1
1:800 1 0
1:1600 0 0
1:4 1 1
1:8 0 0
1:16 0 0
1:32 0 0
1:100 4 3
Anti-D Patient Pool A Dilution Neat 1:2 Exp 1 4 3 Exp 2 4 2
1:1600 1:3200 1:6400 3 1 0 2 1 0
Anti-D Lot 102 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 3 2
1:400 3 1
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 201 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 4 3
1:400 3 2
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 202 Dilution 1:100 Exp 1 3 Exp 2 3
1:200 1 0
1:400 0 0
1:800 0 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 103 Dilution 1:100 Exp 1 4
1:200 4
1:400 3
1:800 3
1:1600 1:3200 1 0
Exp 2
4
Anti-D Lot 203 Dilution 1:200 Exp 1 4 Exp 2 4
4
3
1:400 4 4
1:800 2 3
3
1
0
1:1600 1:3200 1:6400 2 1 0 3 2 1
1:400 3
1:800 2
1:1600 1:3200 1:6400 1 0 0
AHG card Dilution 1:100 Exp1 4
1:400 4
1:800 2
1:1600 1:3200 1:6400 2 1 0
reated cells washed in cellstab AHG card Dilution 1:100 1:400 Exp1 4 3
1:800 2
1:1600 1:3200 1:6400 1 0 0
Pre bromalain treated pooled rr red treated cells Treated date 26/1/09 26/1/09 tdatetest 26/01/09 Test date date 26/1/09 26/1/09 Anti-D 73/515 Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Sal 0 0 0 0 0 0 AHG 0 0 0 0 0 0 Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D 86 i.u. Saline card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 2 1 0 0 0 Exp 2 4 2 1 0 0 0 Exp 3 4 2 1 0 0 0 AHG card Dilution 1:400 Exp 1 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 2 0 0 4 3 2 0 0 3 3 1 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 3 2 1 0 0 Exp 2 4 3 2 1 0 0 Exp 3 4 3 2 1 0 0 Pre Bromalain treated R1R1 pooled Treated cells ate date 26/1/09 26/1/09 9 Test test Test date date date 27/1/09 27/1/09 Anti-D lot 24311 Saline card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 2 0 0 Exp 3 4 4 3 2 0 0
AHG card Dilution 1:4 Exp 1 4 Exp 2 4 Exp 3 4
1:8 4 4 4
1:16 3 3 3
1:32 2 2 3
1:64 1 1 2
1:128 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:4 1:8 1:16 Exp 1 4 3 2 Exp 2 4 3 2 Exp 3 4 3 2
1:32 1 0 1
1:64 0 0 0
1:128 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D Lot 32372B Saline card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 2 1 0 0 Exp 2 4 4 2 1 0 0 Exp 3 4 4 2 1 0 0 AHG card Dilution 1:200 Exp 1 4 Exp 2 4 Exp 3 4
1:400 4 4 4
1:800 4 3 4
1:1600 1:3200 1:6400 2 1 0 2 1 0 2 1 0
Untreated cells in cellstab Standard AHG technique Dilution 1:200 1:400 1:800 Exp 1 4 3 2 Exp 2 4 3 2 Exp 3 4 3 2
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D Lot 101 Saline card Dilution 1:50 1:100 1:200 1:400 1:800 1:1600 Exp 1 4 2 0 0 0 0 Exp 2 4 2 1 0 0 0 Exp 3 4 2 1 0 0 0 AHG card Dilution 1:50 Exp 1 4 Exp 2 4 Exp 3 4
1:100 4 4 4
1:200 2 2 2
1:400 1 0 1
1:800 0 0 0
1:1600 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:50 1:100 1:200 Exp 1 2 2 1 Exp 2 3 2 0 Exp 3 3 2 0
1:400 0 0 0
1:800 0 0 0
1:1600 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 26/1/09 Anti-D Patient Pool A Saline card Dilution Neat 1:2 1:4 1:8 1:16 1:32 Exp 1 2 1 0 0 0 0 Exp 2 2 1 0 0 0 0 Exp 3 3 1 0 0 0 0 AHG card Dilution Neat Exp 1 4 Exp 2 4 Exp 3 4
1:2 2 2 2
Untreated cells in cellstab Standard AHG technique Dilution Neat 1:2 Exp 1 2 1 Exp 2 2 1 Exp 3 2 1
1:4 1 1 1
1:8 0 0 0
1:16 0 0 0
1:32 0 0 0
1:4 0 0 0
1:8 0 0 0
1:16 0 0 0
1:32 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 27/1/09 Anti-D Lot 102 Saline card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 4 3 1 0 0 0 Exp 2 4 4 1 0 0 0 Exp 3 4 3 1 0 0 0 AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4
1:200 3 3 3
1:400 2 2 2
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:100 1:200 1:400 Exp 1 3 2 1 Exp 2 3 2 1 Exp 3 3 2 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 27/1/09 Anti-D Lot 201 Saline card Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600 Exp 1 2 0 0 0 0 0 Exp 2 2 0 0 0 0 0 Exp 3 2 0 0 0 0 0 Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 3 0 0 0 0 0 AHG card Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600 Exp 1 3 2 0 0 0 0 Exp 2 3 2 0 0 0 0 Exp 3 3 2 0 0 0 0 Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 4 2 1 0 0 0 Untreated cells in cellstab Standard AHG technique Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600 Exp 1 2 1 0 0 0 0 Exp 2 2 1 0 0 0 0 Exp 3 2 1 0 0 0 0 Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 4 3 1 0 0 0 Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 27/1/09 Anti-D Lot 202 Saline card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 2 0 0 0 0 0 Exp 2 2 0 0 0 0 0 Exp 3 2 0 0 0 0 0 AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4
1:200 2 2 2
1:400 1* 1* 1*
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:100 1:200 1:400 Exp 1 2 1 0 Exp 2 2 1 0 Exp 3 2 1 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
Pre bromalain treated cells R1R1 pool treated date 26/1/09 test date 27/1/09 Anti-D 73/515 Saline card Dilution 1:100 Exp 1 4 Exp 2 4 Exp3 4
1:400 3 3 3
1:800 3 3 3
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp3 4
1:400 4 4 4
1:800 3 3 3
1:1600 1:3200 1:6400 2 1 0 2 1 0 2 1 0
R1R1 cells in cellstab Dilution 1:100 1:400 Exp 1 4 3 Exp 2 4 3 Exp3 4 3
1:800 2 2 2
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test 2009-1-29 date 27/1/09 Anti-D Lot 103 Saline card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 3 3 2 1 0 0 Exp 2 3 3 2 1 0 0 Exp 3 3 2 2 1 0 0 AHG card Dilution 1:100 Exp 1 4 Exp 2 4 Exp 3 4
1:200 3 3 3
1:400 2 2 2
1:800 2 2 1
1:1600 1:3200 1 0 1 0 0 0
Untreated cells in cellstab Standard AHG technique Dilution 1:100 1:200 1:400 Exp 1 4 4 3 Exp 2 4 4 3 Exp 3 4 3 3
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
Pre Bromalain treated R1R1 pooled Treated cells date 26/1/09 Test date 29/1/09 Anti-D Lot 203 Saline card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 2 1 0
Exp 3
4
4
3
1:400 4 4 4
1:800 4 4 3
1:1600 1:3200 1:6400 3 2 0 2 2 0 2 1 0
Untreated cells in cellstab Standard AHG technique Dilution 1:200 1:400 1:800 Exp 1 4 4 3 Exp 2 4 4 3 Exp 3 4 4 3
1:1600 1:3200 1:6400 2 1 0 2 0 0 2 1 0
AHG card Dilution 1:200 Exp 1 4 Exp 2 4 Exp 3 4
3
1
0
AHG Technique inital titre R1R1 pooled cells Dilution Sample Neat 1:2 1:4 1:8 Lot 101 4 4 4 4 Pat. Pool A 2 1 0 0 Lot 102 4 4 4 4 Lot 201 4 4 4 4 Lot 202 4 4 4 4 Lot 103 4 4 4 4 Lot 203 4 4 4 4
1:16 4 0 4 4 4 4 4
1:32 4 0 4 4 4 4 4
1:64 2 4 4 3 4 4
1:128 1 3 4 2 4 4
1:256 0 2 3 1 3 4
1:512 0 1 2 0 3 4
1:1023 1:2046 0 0 0 1 0 2 3
0 0 0 1 3
Bromelain addition technique Pooled R1R1 cells Pool A Anti-D 73/515 AHG card Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 0 0 0 Exp2 4 3 2 0 0 0 Exp3 4 3 2 0 0 0 Anti-D 86 i.u. AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 1 0 0 0 0 Exp2 4 1 0 0 0 0 Exp3 4 1 0 0 0 0 Anti-D lot 24311 AHG card Dilution 1:4 1:8 1:16 1:32 1:64 1:128 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 32372B AHG card Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 3 2 1 0 0 Exp2 4 3 2 1 0 0 Exp3 4 3 2 1 0 0 Anti-D Lot 101 AHG card Dilution 1:50 1:100 1:200 1:400 1:800 1:1600 Exp 1 3 2 0 0 0 0 Exp2 4 2 0 0 0 0 Exp3 4 2 0 0 0 0 Anti-D Patient Pool A AHG card Dilution Neat 1:2 1:4 1:8 1:16 1:32 Exp 1 2 1 0 0 0 0 Exp2 2 1 0 0 0 0 Exp3 2 1 0 0 0 0 Anti-D Lot 102 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 3 2 1 0 0 0 Exp2 3 2 1 0 0 0 Exp3 3 2 1 0 0 0 Anti-D Lot 201 AHG card Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200 Exp 1 2 1 0 0 0 0 Exp2 2 1 0 0 0 0 Exp3 2 1 0 0 0 0
Anti-D Lot 202 AHG card Dilution 1:100 Exp 1 1 Exp2 1 Exp3 1 Anti-D Lot 103 AHG card Dilution 1:100 Exp 1 4 Exp2 4 Exp3 4 Anti-D Lot 203 AHG card Dilution 1:200 Exp 1 4 Exp2 4 Exp3 4
1:200 0 0 0
1:400 0 0 0
1:800 0 0 0
1:1600 1:3200 0 0 0 0 0 0
1:200 3 3 3
1:400 3 3 3
1:800 1 1 1
1:1600 1:3200 0 0 0 0 0 0
1:400 4 4 4
1:800 3 3 3
1:1600 1:3200 1:6400 1 0 0 1 0 0 1 0 0
Papain treated R1R1 pooled cells Saline cards Anti-D 73/515 Dilution 1:100 Exp 1 4 Exp 2 4 Anti-D 86 i.u. Dilution 1:400 Exp 1 4 Exp 2 4 Anti-D lot 24311 Dilution 1:4 Exp 1 4 Exp 2 4
1:400 3 3
1:800 2 3
1:1600 1:3200 1:6400 1 1 0 2 1 1
1:1600 1:3200 1:6400 1:12800 1:25600 3 1 0 0 0 3 2 0 0 0
1:8 4 4
1:16 3 3
1:32 2 3
1:64 2 1
1:128 1 0
Anti-D Lot 32372B Dilution 1:200 1:400 Exp 1 4 4 Exp 2 4 4
1:800 3 3
Anti-D Lot 101 Dilution 1:50 Exp 1 4 Exp 2 4
1:200 3 2
1:400 1 1
1:800 1 0
1:1600 0 0
1:4 1 1
1:8 0 0
1:16 0 0
1:32 0 0
1:100 4 3
Anti-D Patient Pool A Dilution Neat 1:2 Exp 1 4 3 Exp 2 4 2
1:1600 1:3200 1:6400 3 1 0 2 1 0
Anti-D Lot 102 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 3 2
1:400 3 1
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 201 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 4 3
1:400 3 2
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 202 Dilution 1:100 Exp 1 3 Exp 2 3
1:200 1 0
1:400 0 0
1:800 0 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 103 Dilution 1:100 Exp 1 4
1:200 4
1:400 3
1:800 3
1:1600 1:3200 1 0
Exp 2
4
Anti-D Lot 203 Dilution 1:200 Exp 1 4 Exp 2 4
4
3
1:400 4 4
1:800 2 3
3
1
0
1:1600 1:3200 1:6400 2 1 0 3 2 1
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