Papain Results Collated
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Papain treated R1R1 poolA Saline cards Anti-D 73/515 Dilution 1:100 Exp 1 4 Exp 2 4 Anti-D 86 i.u. Dilution 1:400 Exp 1 4 Exp 2 4 Anti-D lot 24311 Dilution 1:4 Exp 1 4 Exp 2 4
1:400 3 3
1:800 2 3
1:1600 1:3200 1:6400 1 1 0 2 1 1
1:1600 1:3200 1:6400 1:12800 1:25600 3 1 0 0 0 3 2 0 0 0
1:8 4 4
1:16 3 3
1:32 2 3
1:64 2 1
1:128 1 0
Anti-D Lot 32372B Dilution 1:200 1:400 Exp 1 4 4 Exp 2 4 4
1:800 3 3
Anti-D Lot 101 Dilution 1:50 Exp 1 4 Exp 2 4
1:200 3 2
1:400 1 1
1:800 1 0
1:1600 0 0
1:4 1 1
1:8 0 0
1:16 0 0
1:32 0 0
1:100 4 3
Anti-D Patient Pool A Dilution Neat 1:2 Exp 1 4 3 Exp 2 4 2
1:1600 1:3200 1:6400 3 1 0 2 1 0
Anti-D Lot 102 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 3 2
1:400 3 1
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 201 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 4 3
1:400 3 2
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 202 Dilution 1:100 Exp 1 3 Exp 2 3
1:200 1 0
1:400 0 0
1:800 0 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 103 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 4 4
1:400 3 3
Anti-D Lot 203 Dilution 1:200 Exp 1 4 Exp 2 4
1:400 4 4
1:800 2 3
1:800 3 3
1:1600 1:3200 1 0 1 0
1:1600 1:3200 1:6400 2 1 0 3 2 1
Pooled R1R1 cells Pool B Cells Treated 14/2/09 Dilutions made in PBSS Anti-D 73/515 Papainised cell Saline card Test date 19/2/09 Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 2 0 0 Exp 3 4 4 3 2 0 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 2 2 1 Exp 2 4 4 3 3 2 0 Exp 3 4 4 3 3 1 0 Anti-D 86 i.u. Test date 19/2/09 Papainised cell Saline card Test date 19/2/09 Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 3 2 1 0 0 Exp 2 4 3 2 0 0 0 Exp 3 4 4 3 1 0 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 3 1 0 0 Exp 2 4 4 4 1 2 2 Exp 3 4 4 3 2 1 0 Anti-D Lot 32372B Papainised cell Saline card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 4 3 1 0 Exp 2 4 4 3 2 1 0 Exp 3 4 4 4 2 1 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 4 3 2 1 Exp 2 4 4 4 3 2 2 Exp 3 4 4 4 4 3 1 Exp 1 Dilutions made 4 4 3 1 0 0 Exp 2 and discarded 4 4 3 1 0 0 Exp 3 4 4 3 1 0 0
Anti-D Lot 203 Papainised cell Saline card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 3 1 0 Exp 2 4 4 3 3 0 0 Exp 3 4 4 4 3 0 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600 1:51200 Exp 1 4 4 4 4 3 3 Exp 2 4 4 4 3 2 2 Exp 3 4 4 4 3 2 1 Exp 1 Dilutions made 4 3 1 0 0 0 Exp 2 and discarded 4 2 1 0 0 0 Exp 3 4 3 1 0 0 0
1:51200
Pooled R1R1 cells Pool B Test Date 25/2/09 Cell treated 14/2/09 very weak non specific reactions Dilutions made in Albumin saline Anti-D 73/515 Papainised cell Saline card Dilution 1:100 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 3 2 0 0 0 3 1 0 0 0 3 1 0 0 0
Papainised cell AHG card Dilution 1:100 1:400 1:800 Exp 1 Dilutions discarded 3 Exp 2 3 Exp 3 3
1:1600 1:3200 1:6400 1:12800 1:25600 2 1 1 0 0 3 2 1 0 0 3 2 1 1 0
Anti-D 86 i.u. Papainised cell Saline card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 1:512001:102400 Exp 1 Dilutions discarded 4 2 1 1 0 0 Exp 2 4 3 2 0 0 0 Exp 3 4 3 2 1 0 0 Papainised cell AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 1:512001:102400 Exp 1 Dilutions discarded 4 3 2 2 1 0 Exp 2 4 3 2 1 0 0 Exp 3 4 3 2 1 1 0 Anti-D Lot 32372B Papainised cell Saline card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 3 2 1 0 0 4 3 1 0 0 4 3 1 0 0
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 2 1 1 4 3 2 1 1 4 3 2 1 1
Anti-D Lot 203 Papainised cell Saline card Dilution 1:200 1:400 1:800
1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Exp 2 Exp 3
Dilutions discarded 4 4 4
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
4 3 3
3 3 2
1 2 1
0 1 1
0 1 0
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 2 2 1 4 4 3 2 1 4 4 3 2 1
Pooled R1R1 cells Pool B Test date 19/2/09 Treated date 14/2/09 Dilutions made in AB serum Anti-D 73/515 Papainised cell Saline card Dilution 1:100 1:400 1:800 Exp 1 4 4 Exp 2 4 4 Exp 3 4 4
1:1600 1:3200 1:6400 1:12800 3 3 1 0 3 2 1 0 3 2 1 0
Papainised cell AHG card Dilution 1:100 1:400 1:800 Exp 1 4 4 Exp 2 4 4 Exp 3 4 4
1:1600 1:3200 1:6400 1:12800 3 3 1 0 3 3 1 0 3 2 1 0
Anti-D 86 i.u. Papainised cell Saline card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 3 1 0 Exp 3 4 4 3 3 1 0 Papainised cell AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 4 3 2 2 Exp 2 4 4 4 3 2 1 Exp 3 4 4 4 3 2 1 Anti-D Lot 32372B Papainised cell Saline card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 1 0 0 4 3 2 0 0 4 3 2 0 0
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 4 2 1 0 4 4 2 1 0 4 4 3 1 0
Anti-D Lot 203 Papainised cell Saline card Dilution 1:200 1:400 1:800
1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Exp 2 Exp 3
Dilutions discarded 4 4 4
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
4 3 3
3 3 3
2 2 2
1 0 0
0 0 0
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 3 1 1 4 3 2 1 1 4 3 2 1 1
1:400 3 3
1:800 2 3
1:1600 1:3200 1:6400 1 1 0 2 1 1
1:1600 1:3200 1:6400 1:12800 1:25600 3 1 0 0 0 3 2 0 0 0
1:8 4 4
1:16 3 3
1:32 2 3
1:64 2 1
1:128 1 0
Anti-D Lot 32372B Dilution 1:200 1:400 Exp 1 4 4 Exp 2 4 4
1:800 3 3
Anti-D Lot 101 Dilution 1:50 Exp 1 4 Exp 2 4
1:200 3 2
1:400 1 1
1:800 1 0
1:1600 0 0
1:4 1 1
1:8 0 0
1:16 0 0
1:32 0 0
1:100 4 3
Anti-D Patient Pool A Dilution Neat 1:2 Exp 1 4 3 Exp 2 4 2
1:1600 1:3200 1:6400 3 1 0 2 1 0
Anti-D Lot 102 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 3 2
1:400 3 1
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 201 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 4 3
1:400 3 2
1:800 1 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 202 Dilution 1:100 Exp 1 3 Exp 2 3
1:200 1 0
1:400 0 0
1:800 0 0
1:1600 1:3200 0 0 0 0
Anti-D Lot 103 Dilution 1:100 Exp 1 4 Exp 2 4
1:200 4 4
1:400 3 3
Anti-D Lot 203 Dilution 1:200 Exp 1 4 Exp 2 4
1:400 4 4
1:800 2 3
1:800 3 3
1:1600 1:3200 1 0 1 0
1:1600 1:3200 1:6400 2 1 0 3 2 1
Pooled R1R1 cells Pool B Cells Treated 14/2/09 Dilutions made in PBSS Anti-D 73/515 Papainised cell Saline card Test date 19/2/09 Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 2 0 0 Exp 3 4 4 3 2 0 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 2 2 1 Exp 2 4 4 3 3 2 0 Exp 3 4 4 3 3 1 0 Anti-D 86 i.u. Test date 19/2/09 Papainised cell Saline card Test date 19/2/09 Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 3 2 1 0 0 Exp 2 4 3 2 0 0 0 Exp 3 4 4 3 1 0 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 3 1 0 0 Exp 2 4 4 4 1 2 2 Exp 3 4 4 3 2 1 0 Anti-D Lot 32372B Papainised cell Saline card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 4 3 1 0 Exp 2 4 4 3 2 1 0 Exp 3 4 4 4 2 1 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 4 3 2 1 Exp 2 4 4 4 3 2 2 Exp 3 4 4 4 4 3 1 Exp 1 Dilutions made 4 4 3 1 0 0 Exp 2 and discarded 4 4 3 1 0 0 Exp 3 4 4 3 1 0 0
Anti-D Lot 203 Papainised cell Saline card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 Exp 1 4 4 3 3 1 0 Exp 2 4 4 3 3 0 0 Exp 3 4 4 4 3 0 0 Papainised cell AHG card Test date 19/2/09 Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600 1:51200 Exp 1 4 4 4 4 3 3 Exp 2 4 4 4 3 2 2 Exp 3 4 4 4 3 2 1 Exp 1 Dilutions made 4 3 1 0 0 0 Exp 2 and discarded 4 2 1 0 0 0 Exp 3 4 3 1 0 0 0
1:51200
Pooled R1R1 cells Pool B Test Date 25/2/09 Cell treated 14/2/09 very weak non specific reactions Dilutions made in Albumin saline Anti-D 73/515 Papainised cell Saline card Dilution 1:100 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 3 2 0 0 0 3 1 0 0 0 3 1 0 0 0
Papainised cell AHG card Dilution 1:100 1:400 1:800 Exp 1 Dilutions discarded 3 Exp 2 3 Exp 3 3
1:1600 1:3200 1:6400 1:12800 1:25600 2 1 1 0 0 3 2 1 0 0 3 2 1 1 0
Anti-D 86 i.u. Papainised cell Saline card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 1:512001:102400 Exp 1 Dilutions discarded 4 2 1 1 0 0 Exp 2 4 3 2 0 0 0 Exp 3 4 3 2 1 0 0 Papainised cell AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 1:512001:102400 Exp 1 Dilutions discarded 4 3 2 2 1 0 Exp 2 4 3 2 1 0 0 Exp 3 4 3 2 1 1 0 Anti-D Lot 32372B Papainised cell Saline card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 3 2 1 0 0 4 3 1 0 0 4 3 1 0 0
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 2 1 1 4 3 2 1 1 4 3 2 1 1
Anti-D Lot 203 Papainised cell Saline card Dilution 1:200 1:400 1:800
1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Exp 2 Exp 3
Dilutions discarded 4 4 4
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
4 3 3
3 3 2
1 2 1
0 1 1
0 1 0
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 2 2 1 4 4 3 2 1 4 4 3 2 1
Pooled R1R1 cells Pool B Test date 19/2/09 Treated date 14/2/09 Dilutions made in AB serum Anti-D 73/515 Papainised cell Saline card Dilution 1:100 1:400 1:800 Exp 1 4 4 Exp 2 4 4 Exp 3 4 4
1:1600 1:3200 1:6400 1:12800 3 3 1 0 3 2 1 0 3 2 1 0
Papainised cell AHG card Dilution 1:100 1:400 1:800 Exp 1 4 4 Exp 2 4 4 Exp 3 4 4
1:1600 1:3200 1:6400 1:12800 3 3 1 0 3 3 1 0 3 2 1 0
Anti-D 86 i.u. Papainised cell Saline card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 3 2 1 0 Exp 2 4 4 3 3 1 0 Exp 3 4 4 3 3 1 0 Papainised cell AHG card Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 Exp 1 4 4 4 3 2 2 Exp 2 4 4 4 3 2 1 Exp 3 4 4 4 3 2 1 Anti-D Lot 32372B Papainised cell Saline card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 1 0 0 4 3 2 0 0 4 3 2 0 0
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
1:1600 1:3200 1:6400 1:12800 1:25600 4 4 2 1 0 4 4 2 1 0 4 4 3 1 0
Anti-D Lot 203 Papainised cell Saline card Dilution 1:200 1:400 1:800
1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Exp 2 Exp 3
Dilutions discarded 4 4 4
Papainised cell AHG card Dilution 1:200 1:400 1:800 Exp 1 Dilutions discarded 4 Exp 2 4 Exp 3 4
4 3 3
3 3 3
2 2 2
1 0 0
0 0 0
1:1600 1:3200 1:6400 1:12800 1:25600 4 3 3 1 1 4 3 2 1 1 4 3 2 1 1
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