Yuhong Zhang Lab 0209

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Yuhong Zhang 276 Grove Street #.8, Newton, MA, 02466, USA ( 617) 953-4904 (cell); [email protected]

EDUCATION AND ACADEMIC DEGREES 1986-1987, M.Sc.training program in Pharmacology, Anhui Medical University, Hefei, Anhui, P.R.China ( Pharmacology) 1983, Bachelor of medicine, Xian Medical University, Xian, Shaanxi, P.R.China ( Pharmacy)

PROFESSIONAL APPOINTMENTS • • • •

Research associate in Beth Israel Deaconess Medical Center, Jul.2007-now Research fellow, Research associate, in University Texas Medical Branch. USA 1999July.2007 Research administrator in Beijing Biotechnology and New Drugs Center,1997-1999 Lecturer, research fellow of Pharmacology, in Qinghai Medical School 1983 -1997

TECHNICAL SKILLS: My work experience mainly focuses on molecular biology and in vivo pharmacology area.

In Vivo Pharmacology Work: • •

Small animal surgery, collection of blood and various tissue types for ex vivo analysis( primary cell culture, fix tissue for histology work) Animal injections, dosing by using various routes of administration (IV, PO,

SC, IM, ID, IP) •

Animal bleeding, assessment

(clinical observations, health checks, and disease

scoring). • •

Establishing tumor models in mice and rat by injecting tumor cells or performing subcutaneous (SC) implant for cancer pharmacology study Acute, chronic toxicity experiments

Molecular and Cell Biology: • • • • • • • • • • •

Real Time PCR miRNA Isolation of lymphocytes RNA,DNA isolation RNase protection assay Southern blot Northern blot analysis Membrane-based cDNA macroarrays Cell culture (primary cell culture) Stable transfection Two-Dimensional Gel Electrophoresis

Immunology, Antibody and Proteins: • • •

Immunohistochemical staining Elisa assay Western blot

Laboratory management • • • • • •

Clinical samples collection and shipment Serum bank and tissue bank maintenance Data analysis, and familiar with bioinformatics Order lab supply Lab equipments maintenance Protocol creating and modification

Computer Skills •

Microsoft Office XP (Word, Excel, PowerPoint, Access).

PUBLICATIONS AND PRESENTATIONS: •





Yuhong Zhang et al: Ribavirin Treatment Up-regulates Antiviral Gene _Expression via the Interferon-Stimulated Response Element in Respiratory Syncytial Virus-Infected Epithelial Cells. Journal of Virology, May.2003, p.5933-5947 Yuhong Zhang et al: _Expression of Respiratory Syncytial Virus-Induced Chemokine Gene Networks in Lower Airway Epithelial Cells Revealed by cDNA Microarrays. Journal of Virology, Oct.2001, p.9044-9058 Daryl Lau, Mala Sinha, Bruce Luxon, Yuhong Zhang et al: Interferon-induced Gene Expression in Liver and Peripheral Blood Mononuclear cells(PBMCs) in HCV Genotype 1





• • • • •

patients. Abstract on 15th International Symposium on Hepatitis C Virus & Related Viruses 2008. Brasier AR, Spratt H, Wu Z, Boldogh I, Zhang Y, et al: Nuclear Heat Shock Response and Novel Nuclear Domain 10 Reorganization in Respiratory Syncytial Virus-infected A549 Cells Identified by High-Resolution Two-Dimensional Gel Electrophoresis. Journal of Virology, Nov.2004, p.11461-11476 Tian B, Zhang Y, Luxon BA, Garofalo RP, Casola A, Sinha M, Brasier AR. Identification of NF-kappaB-dependent gene networks in respiratory syncytial virus-infected cells. Jounal of Virology, Jul.2002, p.824-836 Yuhong Zhang et al: Measurement of hair vanadium in patients with heart, haptic and renal dysfunction. Journal of Qinghai Medical College 1998,19(1):1 Yuhong Zhang: Anti-HIV effect of Fluoroquinolones. Journal of Chinese Northwest Pharmcology 1997,2:88 Yuhong Zhang et al: Measurement of hair vanadium in acute leukemia. Journal of Qinghai Medical College 1996,1:18 Yuhong Zhang et al: Development and application of ion pump inhibitor. Journal of Qinghai Medicine 1994,5:88 Yuhong Zhang: ACEI is benefit for patients with acute heart infarction. Journal of Qinghai Medical College 1993,14(2):122

Textbook: • •

Advance pharmacology course, 3rd,Shaanxi people's educational press, 1995 Apply clinic pharmcology,1st, Gansu culture press, 1996

Reference: Available upon request

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