Urea Uv.pdf
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UREA/BUN
Enzymatic-spectrophotometric ULTRAVIOLET
The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.
Instrument:
AU 400 / 640
Reagent preparation
Working Reagent: Transfer the contents of one Reagent B vial into a Reagent A bottle. Stable for 2 months at 2-8 ºC.
Instrument settings Specific Test Parameters Sample: Volume 2 µL Reagents: R1 Volume 300 µL Wavelength: Pri. 340 Method: FIX Reaction slope: Measuring Point 1: First 2 Measuring Point 2: First 0 Linearity: % No Lag Time:
Min OD
First L -0.1 First H 0.5 Last L -0.1 Last H 0.5
Dynamic Range:
Last 5 Last 0
Correlation Factor:
L
0 H
300
A
1 B
0
On-board stability period:
7. None selected 8. Out of range L
Panic Value
Max OD H 2.5
L -0.1 Reagent OD limit:
Dilution 0 µL Dilution 0 µL Sec. 380
H
L
H
10
50
Unit: mg/dL
Decimal places: 0
Counts:
Process: CONC.
Calibration Specific Calibration Type: Point 1:
AB
Formula: Cal. Nº #
Y=AX+B OD
CONC *
2 Factor/OD-L -9999
Factor/OD-H 9999
* Enter the assigned standard value # User defined Vers. 0206
Enzymatic-spectrophotometric ULTRAVIOLET
The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the instrument. It is advisable to validate the use when there is any change in software or reagent versions.
Instrument:
AU 400 / 640
Reagent preparation
Working Reagent: Transfer the contents of one Reagent B vial into a Reagent A bottle. Stable for 2 months at 2-8 ºC.
Instrument settings Specific Test Parameters Sample: Volume 2 µL Reagents: R1 Volume 300 µL Wavelength: Pri. 340 Method: FIX Reaction slope: Measuring Point 1: First 2 Measuring Point 2: First 0 Linearity: % No Lag Time:
Min OD
First L -0.1 First H 0.5 Last L -0.1 Last H 0.5
Dynamic Range:
Last 5 Last 0
Correlation Factor:
L
0 H
300
A
1 B
0
On-board stability period:
7. None selected 8. Out of range L
Panic Value
Max OD H 2.5
L -0.1 Reagent OD limit:
Dilution 0 µL Dilution 0 µL Sec. 380
H
L
H
10
50
Unit: mg/dL
Decimal places: 0
Counts:
Process: CONC.
Calibration Specific Calibration Type: Point 1:
AB
Formula: Cal. Nº #
Y=AX+B OD
CONC *
2 Factor/OD-L -9999
Factor/OD-H 9999
* Enter the assigned standard value # User defined Vers. 0206
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