Tissue Culture Proposal For Ecb - Copy.docx
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MATERIALS AND SUPPLIES APPENDIX TABLE 1: A: LABORATORY APPARATUS AND EQUIPMENTS (FOR MEDIA PREPARATION) MATERIALS AND SUPPLIES 1. pH meter 2. Top loading digital weighing scale 3. Hot plate magnetic stirrer 4. Magnetic stirring bar 5. Magnetic retriever 6. Graduated cylinder 7. Graduated cylinder 8. Graduated cylinder 9. Beaker 10. Beaker 11. Beaker 12. Amber bottles 13. 14. 15. 16. 17. 18.
Refrigerator Sterilizer Pressure cooker (U.S. Made) Fire extinguisher Air conditioning units Casserole 1 set
19. Gas stove two burners La Germania 20. LPG tank with content 21. LPG tank refill 22. Stirring rods 12” 23. Automizer / hand sprayer 24. Plastic trays 25. Laboratory Gowns 26. 27. 28. 29. 30. 31. 32. 33. 34. 35.
Head cap Slippers household gloves Apron Disposable lighter Detergent powder Aluminum foil Alcohol 70% Dispensing table Pail with cover Small
CAPACITY/ SPECS. Handy 1000g x 0.0001 capacity 12” x 5/16 12” long 10 ml glass 100 ml glass 1000 ml glass 1000 ml glass 2000 ml glass 5000 ml glass 2.0 liter
Quantity 1 1 1 2 1 3 12 3 12 12 3 12
7 feet 1 pc 40 liters 6.5 kgs 2.0 HP Stainless/food grade
1 1 1 1 1 1
Heavy duty
1
50 kgs 50 kgs 2 pieces 3 pieces 25 pieces Color white/ long sleeves Disposables Plastic Disposable Cloth
1 1 2 3 25 6
50 kilograms/sack 8 “5m Galloon
4
6 5 6 3 1 box 6 10 rolls 6 1 4
Price
Amount
36. 37. 38. 39. 40. 41. 42.
Medium Large Pipette 1ml 0.5 ml Culture bottles small w/cover Culture bottles big w/ cover Volumetric flask 1000ml Volumetric flask 2000ml Weighing boat Spatula for reagents TOTAL
3 2 3 3 5,000 10,000 2 1 1 1 set
3 2 5,000 10,000 2 1
APPENDIX TABLE 1: B MATERIALS FOR INOCULATION ROOM Materials 1. Wooden chamber set 2. Aircon (Split type) 3. Computer chair for chambers 4. Surgical blades Japan 5. Forceps: scissors forcep 6. Thumb forcep 10” 7. Scalpel 4” 8. Lamps 9. Laboratory gown 10. Automizer/ sprayer 11. Denatured alcohol 12. Head cap and mask 13. Slippers 14. Rubbing alcohol 15. Scalpel and forcep holders 16. Carts (2 layers) 17. Lighter 18. Chamber arm cover TOTAL
Quantity 3 1 3 1 box 3 3 3 3 6 3 6 galloons 6 sets 4 sets 12 bots 3 pieces 3 1 box 6 pairs
Price
Amount
APPENDIX TABLE 1: C MATERIALS FOR CULTURE ROOM Materials 1. 2. 3. 4.
Shelves Fluorescent lamps Aircon Ladder
Specifications 6x2x6’ (10pcs) 20 watts (100 pcs) 2.0 HP 6’
Price 5,500.00 250.00 25,000.00 3,000.00
Amount 55,000.00 25,000.00 25,000.00 3,000.00
TOTAL
108,000.00
APPENDIX TABLE 1: D MATERIALS FOR SUCKER PREPARATION
Materials 1. Chopping board 2. Corm /Sucker 3. Knife big and small 4. Zonrox bleach 5. Powder detergent 6. Pails
7. 8. 9. 10.
Aluminum foil Slashing bolo Tumbling bolo Cotton TOTAL
Specs Wooden and plastic heavy duty 1000 pcs Stainless 6 gals 50 kilograms/sack 5 liter 10 liter 15 liter 5 rolls 1 1 rolls
Price 10.00
Amount 10,000.00
APPENDIX TABLE 1: E MATERIALS FOR MEDIA PREPARATION Materials 1. Dispensing Table 2. Plastic Chairs 3. Funnels (for dispensing) 4. Trays for (media bots)
Quantity 1 6 3
Price 3,500.00 600.00 25.00
Amount 3,500.00 3,600.00 150.00
4
250.00
1,000.00
TOTAL
8,250.00
Price
Amount
APPENDIX TABLE 1: E MISCELLANEOUS SUPPLIES Materials 1. Ball pen BBGR 2. Pentel Pen BB 3. Record books 4. Scotch Tape 5. Packaging Tape 6. Broom 7. Mop 8. Shoe racks 9. Tissue paper 10. Plastic bags
Quantity 4 boxes 2 boxes 2 2 rolls 2 rolls 3 pieces 3 pieces 2 pieces 12 rolls 1 roll
11. Gauge Bandage 12. Office supplies
1 roll
TOTAL APPENDIX TABLE 2: CHEMICALS TO BE USED IN THE TISSUE CULTURE LABORATORY Name of Chemicals Ammonium nitrate Potassium Iodide Cobalt Chloride Boric Acid Manganese Sulfate monohydrate Zinc Sulfate Sodium Molybdate Nicotinic acid Pyridoxine HCl Activated Charcoal Powder Sodium Hydroxide Buffer Solution pH4 Formalin (Formaldehyde) Potassium permanganate Agar Ethyl alcohol Edta (Sequestrine agar) IAA Glycerine Copper Sulphate
Grade AR AR AR AR AR
Packing 1 kilo 500g 100g 500g 500g
AR AR AR AR AR AR AR AR AR AR AR AR AR AR AR
500g 100g 100g 25g 500g 1 kilo 1 liter 2.5 liter 500g 5 kilos 2.5 liter 250 g 5g 2.5 500 g
Schedule of Activities Q1
Year 1 Q2 Q3
Year 2
Year 3
Q4
1. Reconnaissance and Site inspection 2. Site preparation and delineation 3. Laboratory supplies/equipment acquisition 4. Laboratory Set-up/ construction of shelves 5. Preparation of Culture media 6. Collection of Explants 7. Inoculation of explants 8. Incubation of plantlets 9. Potting out of explants 10. Recovery and hardening stage 11. Disposal for Marketing
BUDGETARY REQUIREMENTS: ‘000 Items
Year 1 Q1
Q2
Q3
60,000
60,000
60,000
91,494
91,494
Q4
Total
Year 2
Total
1. Salary a) Honorarium 20,000 per month b) Propagator 3x 391x26x3 c) Nursery Aide 250x26x3 d) Lab aid 3x350x26x3
81,900
81,900
180,000
180,000
91,494
274,482
365,976
640,458
19,500
19,500
78,000
175,500
81,900
245,700
245,700
737,100
SUB-TOTAL 2. Laboratory Apparatus/Equipt. 3. Supplies and materials 4.Chemicals/Reagents Grand Total
Refrigerator Sterilizer Pressure cooker (U.S. Made) Fire extinguisher Air conditioning units Casserole 1 set
19. Gas stove two burners La Germania 20. LPG tank with content 21. LPG tank refill 22. Stirring rods 12” 23. Automizer / hand sprayer 24. Plastic trays 25. Laboratory Gowns 26. 27. 28. 29. 30. 31. 32. 33. 34. 35.
Head cap Slippers household gloves Apron Disposable lighter Detergent powder Aluminum foil Alcohol 70% Dispensing table Pail with cover Small
CAPACITY/ SPECS. Handy 1000g x 0.0001 capacity 12” x 5/16 12” long 10 ml glass 100 ml glass 1000 ml glass 1000 ml glass 2000 ml glass 5000 ml glass 2.0 liter
Quantity 1 1 1 2 1 3 12 3 12 12 3 12
7 feet 1 pc 40 liters 6.5 kgs 2.0 HP Stainless/food grade
1 1 1 1 1 1
Heavy duty
1
50 kgs 50 kgs 2 pieces 3 pieces 25 pieces Color white/ long sleeves Disposables Plastic Disposable Cloth
1 1 2 3 25 6
50 kilograms/sack 8 “5m Galloon
4
6 5 6 3 1 box 6 10 rolls 6 1 4
Price
Amount
36. 37. 38. 39. 40. 41. 42.
Medium Large Pipette 1ml 0.5 ml Culture bottles small w/cover Culture bottles big w/ cover Volumetric flask 1000ml Volumetric flask 2000ml Weighing boat Spatula for reagents TOTAL
3 2 3 3 5,000 10,000 2 1 1 1 set
3 2 5,000 10,000 2 1
APPENDIX TABLE 1: B MATERIALS FOR INOCULATION ROOM Materials 1. Wooden chamber set 2. Aircon (Split type) 3. Computer chair for chambers 4. Surgical blades Japan 5. Forceps: scissors forcep 6. Thumb forcep 10” 7. Scalpel 4” 8. Lamps 9. Laboratory gown 10. Automizer/ sprayer 11. Denatured alcohol 12. Head cap and mask 13. Slippers 14. Rubbing alcohol 15. Scalpel and forcep holders 16. Carts (2 layers) 17. Lighter 18. Chamber arm cover TOTAL
Quantity 3 1 3 1 box 3 3 3 3 6 3 6 galloons 6 sets 4 sets 12 bots 3 pieces 3 1 box 6 pairs
Price
Amount
APPENDIX TABLE 1: C MATERIALS FOR CULTURE ROOM Materials 1. 2. 3. 4.
Shelves Fluorescent lamps Aircon Ladder
Specifications 6x2x6’ (10pcs) 20 watts (100 pcs) 2.0 HP 6’
Price 5,500.00 250.00 25,000.00 3,000.00
Amount 55,000.00 25,000.00 25,000.00 3,000.00
TOTAL
108,000.00
APPENDIX TABLE 1: D MATERIALS FOR SUCKER PREPARATION
Materials 1. Chopping board 2. Corm /Sucker 3. Knife big and small 4. Zonrox bleach 5. Powder detergent 6. Pails
7. 8. 9. 10.
Aluminum foil Slashing bolo Tumbling bolo Cotton TOTAL
Specs Wooden and plastic heavy duty 1000 pcs Stainless 6 gals 50 kilograms/sack 5 liter 10 liter 15 liter 5 rolls 1 1 rolls
Price 10.00
Amount 10,000.00
APPENDIX TABLE 1: E MATERIALS FOR MEDIA PREPARATION Materials 1. Dispensing Table 2. Plastic Chairs 3. Funnels (for dispensing) 4. Trays for (media bots)
Quantity 1 6 3
Price 3,500.00 600.00 25.00
Amount 3,500.00 3,600.00 150.00
4
250.00
1,000.00
TOTAL
8,250.00
Price
Amount
APPENDIX TABLE 1: E MISCELLANEOUS SUPPLIES Materials 1. Ball pen BBGR 2. Pentel Pen BB 3. Record books 4. Scotch Tape 5. Packaging Tape 6. Broom 7. Mop 8. Shoe racks 9. Tissue paper 10. Plastic bags
Quantity 4 boxes 2 boxes 2 2 rolls 2 rolls 3 pieces 3 pieces 2 pieces 12 rolls 1 roll
11. Gauge Bandage 12. Office supplies
1 roll
TOTAL APPENDIX TABLE 2: CHEMICALS TO BE USED IN THE TISSUE CULTURE LABORATORY Name of Chemicals Ammonium nitrate Potassium Iodide Cobalt Chloride Boric Acid Manganese Sulfate monohydrate Zinc Sulfate Sodium Molybdate Nicotinic acid Pyridoxine HCl Activated Charcoal Powder Sodium Hydroxide Buffer Solution pH4 Formalin (Formaldehyde) Potassium permanganate Agar Ethyl alcohol Edta (Sequestrine agar) IAA Glycerine Copper Sulphate
Grade AR AR AR AR AR
Packing 1 kilo 500g 100g 500g 500g
AR AR AR AR AR AR AR AR AR AR AR AR AR AR AR
500g 100g 100g 25g 500g 1 kilo 1 liter 2.5 liter 500g 5 kilos 2.5 liter 250 g 5g 2.5 500 g
Schedule of Activities Q1
Year 1 Q2 Q3
Year 2
Year 3
Q4
1. Reconnaissance and Site inspection 2. Site preparation and delineation 3. Laboratory supplies/equipment acquisition 4. Laboratory Set-up/ construction of shelves 5. Preparation of Culture media 6. Collection of Explants 7. Inoculation of explants 8. Incubation of plantlets 9. Potting out of explants 10. Recovery and hardening stage 11. Disposal for Marketing
BUDGETARY REQUIREMENTS: ‘000 Items
Year 1 Q1
Q2
Q3
60,000
60,000
60,000
91,494
91,494
Q4
Total
Year 2
Total
1. Salary a) Honorarium 20,000 per month b) Propagator 3x 391x26x3 c) Nursery Aide 250x26x3 d) Lab aid 3x350x26x3
81,900
81,900
180,000
180,000
91,494
274,482
365,976
640,458
19,500
19,500
78,000
175,500
81,900
245,700
245,700
737,100
SUB-TOTAL 2. Laboratory Apparatus/Equipt. 3. Supplies and materials 4.Chemicals/Reagents Grand Total
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