Sujit

A SEMINAR ON: SEROLOGICAL REACTION Submitted By: Mr.SUJIT J. TAMHANE For The Year 2006-07

ACKNOWLEDGEMENT I am greatful to our trustee Mr. Tekchand Shah for giving me the opporturnity to do this project work. I am greatful to our respected principle Dr. (Mrs)Sudha Rathod for giving me encouragement & inspiration. I am also thankful to Mrs. Smita mahajan (Project Incharge) for giving me valuable guidance in the preparation of this project work. I thanks to all faculty members, librarian, and my friends for giving me helps to me complete this project.

 DEFINATION OF SEROLOGICAL REACTIONS: FEATURES OF ANTIGENANTIBODY REACTION :

FOLLOWING TYPES OF SEROLOGICAL REACTION

:

1.NEUTRALISATION REACTION 2.PRESIPITAION

REACTION

3.AGLUTINATION REACTION 4.FIOCULATION REACTION 5.COMPLIMENT FIXATION

1.NEUTRALIZATION REACTIONS : 2.PRECIPITIN REACTIONS :

3.FLOCCULATION REACTIONS :

4.AGGLUTINATION REACTIONS : 5.COMPLEMENT FIXATION TEST:

ENZYME – LINKED IMMUNOSORBENT ASSAY (ELISA) :

ELISA PLATE :

WESTERN BLOT :

ELECTROPHORESIS

IMMUNOELECTROPHORESIS : IMMUNOFLUORESCENCE :

Antigen detection :Agar-gel immunodiffusion (AGID) Counterimmunoelectrophoresis (CIEP) Passive haemagglutination (PHA) Immunofluorescence (IF) Immunoperoxidase staining (IPS) Immunocapture ELISA Electron microscopy (EM) Polymerase chain reaction (PCR)

1.AGAR-GEL IMMUNODIFFUSION (AGID) :

2.COUNTERIMMUNOELECTROP HORESIS(CIEP) :

RESULT

3.IMMUNOFLUORESCENCE (IF) : METHOD: DIRECT IMMUNOFLUORESCENCE

A

B

C

INDIRECT IMMUNOFLUORESCENCE :

4.ELECTRON MICROSCOPY (EM) : METHODS ASSESSMENT OF RESULT 5.POLYMERASE CHAIN REACTION (PCR) :