CLINICAL LABORATORY 1. What is the function of screening? ➢ Detect subclinical disease ➢ Detect and quantify the risk of future disease 2. Role of the laboratory test
➢ Absolutely specific for a specific disease ➢ Easily measurable ➢ Early detection ➢ Not affected by other biological disturbances 7. Function of Troponin 1 is a
➢ Screening
marker of MI because
➢ Diagnoses
➢ Found predominately in
➢ Helpful patient management 3. What are the steps in investigation of a patient? ➢ Imaging techniques ➢ diagnosis ➢ Evaluation 4. Why are laboratory tests
cardiac tissue ➢ Released into the blood stream following cell death ➢ Highly specific marker 8. Inter-individual varation ➢ Age ➢ Sex
ordered?
➢ Race
➢ Prevent malpractice
➢ Genetic
➢ Educating residents
➢ Long term health issue
➢ Monitor effectiveness!! ➢ Identify complication of treatment ➢ Check accuracy and unexpected data 5. What are normal biological specimens?
BUC (Comprise the majority of ➢ Blood all specimens ➢ Urine analyzed) ➢ Cerebrospinal fluid
9. Intra-individual variation ➢ Diet ➢ Exercise ➢ Drugs ➢ Time of venipucture ➢ Length of time tourniquet is applied ➢ Posture ➢ Sleep pattern 10.Pre-analytical variation ➢ Transport
➢ Stools
➢ Exposure to UV light
➢ Tissue specimen
➢ Storage condition
6. What is the function of a
➢ Centrifugation time
marker? Ps (Ten): Just can only know these questions… Anywhere, Others question all in pictures form lo!! All the best guy~ Good lUck Page 1
CLINICAL LABORATORY ➢ Standing time be4 separation of cell
➢ Increases Potassium, Magnesium and
11.Analytical variation
phosphorous
➢ Random errors
17.Lipemia and proteinemia
➢ Systemic errors 12.Post-analytical ➢ Transcription errors ➢ Result reported to wrong patient 13.Prolonged venous stasis
cause: ➢ Serum and plasma to become turbid!! 18.Blood composition with anticoagulant (Chelating agent EDTA, Citrate /
(tourniquet put in a long
Heparin enhance
period) will cause?!
antithrombin)!
➢ Increase( Total
➢ 3 layers (PLASMA,
protein, Proteins, iron Fe, Cholesterol, and bilirubin) ➢ Decreases potassium (K+) 14.Supine position ( reduces
BUFFY COAT, and RBC) ➢ Buffy coat (WBC and platelets) ➢ Plasma (55% whole blood – protein, sugar, vitamin,
700ml blood volume)
mineral, lipids,
➢ Decrease 5-15% of
lipoprotein, and clotting
(Total protein,
factors)
albumin, lipids, iron,
(95% of plasma is
Calcium and
water)
Enzymes) 15.Specimens which required
19.Blood composition without anticoagulant
special handling technique:
➢ 2 layers
➢ Lactate
➢ Serum and blood clot
➢ Ammonia
➢ Serum same as plasma
➢ Acid phosphatase
but without clotting
➢ Plasma catecholamines
factors
16.Hemolysis will cause: ➢ Serum and plasma into red
➢ Blood clot (Clotting factors, fibrin, platelets, and RBCs) 20.Collection tube
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CLINICAL LABORATORY ➢ Red (X anticoagulant)
• Urea; BUN; Creatinine
➢ Gold (Gel barrier)
• GFR
➢ Grey (Glucose)
➢ Endocrine (DM)
➢ Green (Anticoagulant) ➢ Lavender (EDTA; Inhibit coagulation) ➢ Light blue (Coagulation studies) ➢ Brown and Royal Blue (Trace metal studies) 21.Complete blood count is? ➢ Haemoglobin ➢ WBC count ➢ Platelets count ➢ MCV ➢ MCH
DIAGNOSA • fasting glucose • 2h post prandial glucose • OGTT • C-peptide (DD type 1 and type 2 DM) MONITORING • C.B.C, Urinalisa • Lipid profile
➢ MCHC
• Renal function
➢ RDW
• Electrolyte
➢ Haematocrit
• Blood gas analysis
➢ RBC count
• Ketone bodies
➢ Erythrocyte
• Hb A1C
sedimentation rate ➢ Blood smear evaluation 22.POCT (Point of care testing) ➢ Blood glucose
• Fructosamine • Micro-albuminuria • Insulin-Fasting and 2 h pp
➢ Urinalysis ➢ Blood gases ➢ Electrolytes ➢ Cardiac markers (troponin 1 and T) ➢ Drugs screen 23.Routine clinical chemistry in laboratory examination ➢ Renal function test
➢ Lipid profile • Total cholesterol • Trygliceride • HDL • LDL • Lp(a) • ApoA, ApoB
• Urinalysis Ps (Ten): Just can only know these questions… Anywhere, Others question all in pictures form lo!! All the best guy~ Good lUck Page 3
CLINICAL LABORATORY ➢ Liver function test Hepatobilier
➢ Hematuria ➢ Myohemoglobinuria???
function • Synthesis function:
肌红蛋白尿症 ➢ Myoglobinuria??
CHE, albumin, coagulation factors
肌红蛋白尿 ➢ Hemoglobinuria??
• Hepatocellular functions: AST, ALT • Obstructive functions: GGT, ALP • Excretions: Bilirubin,
血红素尿 28.Observe the tube with graphic paper background is to? ➢ Observe the clarity of the urine
bile acid 24.Markers for hepatitis:
29.Clarity of urine influenced
➢ AFP
by
➢ Ceruloplasmin
➢ Leucocyturia
➢ Serum iron
➢ hematuria
➢ Feritin
➢ Bacteriuria
25.Type of macroscopic examination ➢ Colour ➢ Clarity
➢ Mucus from vagina ➢ Phosphate precipitation 30.Dipstick examination: ➢ Provide information-
➢ Volume (count by
• Carbohydrate
urinometer)
metabolism
➢ pH
• Kidney function
➢ odor
• Liver function
26.Chemical examination ➢ Glucose ➢ Blood (haemoglobin) ➢ Leukocyte esterase ➢ Protein ➢ Urobilinogen ➢ Bilirubin ➢ Ketone ➢ Nitrite 27.Red urine is caused by
• Acid-base balance • UTI Dip not longer than 1 second
31.Indicator used in the dipstick: ➢ Glucose (Blue-brown) • Glucosa oxidase
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CLINICAL LABORATORY • Peroxidase ➢ Bilirubin (Yellow-brown) • Diazotized dicholororaniline salt ➢ Ketone • Nitroprusid ➢ SG
➢ Acid sulfosalisilat ➢ Protein Bence Jones • +ve or –ve 33.Glucose test ➢ Benedict (5 ml) and 8 drops (1/10 of urine) • Negative (Blue) • +1 (Bight yellowish)
• Bromthymol blue
• +2 (Yellow + green)
• Polyelectrolyte
• +3 (Orange)
(Polymethylvinyl ether/ maleic acid) ➢ Blood (Yellow-green) • Tetramethylbenzidine ➢ pH
• +4 (Brownish red) ➢ Reduction test Others redactor: • Vit C • Lactose
• Bromthymol blue
• Asam salisilat
• Methyl red
• Galactose
➢ Protein (Green) • Tetrablomphenol
• Fructose 34.Picture!!
➢ Urobilinogen (Red)
➢ Erythrocyte
• Diazonium salt
➢ Leukocytes
➢ nitrite (pink) • P-arsanilic acid ➢ Leucocyte • Indoxyl ester 32.Urine protein test ➢ 2ml of urine + 3-5 drops acetic acid
➢ Hyaline cast ➢ Erythrocyte cast • Glomerulonephritis ➢ Leukocyte cast • Pyelonephritis ➢ Granular cast (Coarsely) 35.Oval Fat bodies +
• Negative
➢ Nephrotic syndrome
• +1 white
➢ Tubular cells that have
• +2 white cloudy
absorbed lipoproteins
• +3 white yellowish
with cholesterol and
• +4 white yellowish
triglycerides leaked
cloudy
from nephritic glomeruli
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CLINICAL LABORATORY 36.Leukocyte cast + ➢ Pyelonephritis 37.Erythrocyte cast + ➢ GLomerulonephritis 38.Fever ➢ Upper UTI 39.Burning sensation ➢ Lower UTI
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