Role Of Eclipta Alba

ROLE OF ECLIPTA ALBA CSA IN COMBINATION TO 17kDa LEISHMANIA DONOVANI POLYPEPTIDE IN MACROPHAGE ACTIVATION AND ITS LEISHMANICIDAL FUNCTION Shubhankar K Singha, Sanjiva Bimala, Raageeba Bimalb, PK Sinhaa, Ravi Rajhansa and P Dasa. a. Rajendra Memorial Research Institute of Medical Sciences (Indian Council of Medical Research) Agamkuan, Patna, 800007, India. b. Biotechnology Lab, Post Graduate Department of Botany, BRA Bihar University, Muzaffarpur, Bihar, India. Abstract: Eclipta alba induced significant TNF-α and nitric oxide synthesis in combination to 17kDa polypeptide from macrophages of Kala-azar patients. Introduction: Macrophage infected with Leishmania donovani fails to express inducible nitric oxide synthase (iNOS) and hence down regulating nitric oxide (NO) production, which is a microbicidal component of infected macrophage. Its expression is induced by the secretion of an array of Th1 cell response and synthesis of TNF-α from infected macrophages. In the present study, we targeted lectin receptor to internalize plants extracts with 17kDa polypeptide to explore its possible role in induction of leishmanicidal components inside. Objectives: To trigger anti-leishmanicidal effect in macrophages of visceral leishmaniasis through lectin receptor with plant extract in combination to 17kDa polypeptide. Materials and methods: T-cell depleted macrophage culture of VL patients pre-pulsed with L. donovani promastigotes were incubated with CSA obtained from E. alba or A. indica alone or in presence of 17kDa polypeptide. Control wells were arranged with or without 17kDa polypeptide. NO were measured in culture supernatant using Griess reagent, however TNF-α accumulated macrophages were stained with anti human TNF-α FITC and measured on Flow cytometer. Results and Discussion: Both the plant CSA and 17kDa polypeptide induced higher percentage of TNF-α and corresponding NO level. But the CSA obtained from A. indica failed to express its adjuvant capability as the level of NO released in combination to 17kDa was comparable to the level induced by either alone. However, the CSA obtained from E. alba in combination to 17kDa protein fraction expressed four fold higher amount than that of either alone. The present study defines the possible leishmanicidal role of CSA obtained from E. alba in combination to 17kDa polypeptide. The possibility that CSA of E. alba acts by upregulating TNF-α and NO expression in macrophages infected with Leishmania donovani needs to be explored further in order ascertain their therapeutic capabilities in VL patients. Referances: 1. Shubhankar K Singh, Sanjiva Bimal, Diwakar S Dinesh, Anil K Gupta, Prabhat K Sinha, Raajeeva Bimal and Pradeep Das. 2005. Towards identifying immunogenic targets in visceral leishmaniasis: role of 17kDa and 63kDa phosphoproteins. American Journal of Immunology 1(3): 94-98. 2. Liew FY. 1984. Regulation of nitric oxide synthesis in infection and autoimmune disease. Immunol. Lett. 43: 95-98.