Pour Gels 1. Make Sure That The Gel Tray

Pour gels 1. Make sure that the gel tray is sealed with the blocks or with 2-3 pieces of tape 2. Place 1-2 combs in the gel 3. Pour liquid molten agarose into the gels up to just below the top of the comb teeth 4. Let cool Extract “DNA” and cut with restriction enzymes 1. Place “DNA” samples in labeled test tubes with 1 mL of hot water 2. Agitate 3. Place in boiling water bath to concentrate the sample Loading the Gels 1. Gently remove the combs 2. Set the micropipette to 20 uL. 3. Using a new sterile tip for each well place 20 uL of sample going from left to right. Label which wells get which sample below. Everyone should practice loading a few wells Run the Gels 1. Set the gel tray near the power supply 2. Pour running buffer into the tray 3. Gently place the gel into the tray with the samples near the ________________ end (because DNA is ______________) 4. Plug in the tray. Check for bubbles on the electrodes (this shows current is flowing and causing electrolysis of water) 5. Let it run for about 30 minutes and check to see if the bands have separated enough Gel Diagram