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POSTGRADUATE COURSEWORK STUDENT COMMUNITY (BACS80C_5820_POSTGRADUATE_COURSEWORK) > TAKE ASSESSMENT: QUIZ

Take Assessment: Quiz Name Quiz Instructions Answer all questions. Final date for completing the quiz is 17 August 2007. Multiple Attempts Not allowed. This Test can only be taken once. Force Completion This Test can be saved and resumed later.

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Question 1

1 points

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Recombinant DNA molecules usually contain the DNA from at least three genomes. For insect cells containing baculovirus with a plant defensin gene, indicate the host, vector, donor and insert. Baculovirus

Plant

Defensin gene

Insect cells

A. Ve ct or B. H os t C. In se rt D. D on or

Question 2

1 points

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For the human insulin gene in pBR322 growing in E.coli indicate the host, vector, donor and insert. Human

Insulin gene

pBR322

E. coli

A. H os t B. Ve ct or C. D on or D. In se rt

Question 3

1 points

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For E. coli containing a BAC with an insert of yeast genomic DNA indicate the host, vector, donor and insert. E.coli

Bacterial Artificial Chromoso me (BAC)

Yeast

Genomic DNA

A. H os t B. D on or C. Ve ct or D. In se rt

Question 4

1 points

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For pGEX in mouse PC12 cells expressing jelly fish yellow fluorescent protein indicate the host, vector, donor and insert. pGEX

Mouse

Yellow fluorescent protein gene

Jellyfish

A. H os t B. D on or C. In se rt D. Ve ct or

Questi on 5

1 points

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Design a forward primer of 20 base pairs to amplify by PCR the 100 base pair fragment shown in bold in the following DNA sequence. CTGTGAAAGGGTCATTTGACTCCCAAAAGGGTCACGACCCATTGAGAACAACTGATGTAGA

CAGTGTATGAACATTCT TGCTAGTACTTGTGTGGTTAATGAGTTTGCTTTGAGCCATTTGAA TAGCTGTATAATAACAC ACTGTATAGTCTTAATTGCTATGTTCGTGTCTCATGATACTGAATATTTTTGTGT GTTTACTTGCCAAGTGTATTTTT TAAAACTTGTTTTTTTGCCTATTTTCAAGTGAAAATTCATGTTACTGTCCTTTTCATTAAT AAATAGATGTACACTAA

Questi on 6

2 points

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Design a reverse primer of 20 base pairs to amplify by PCR the 100 base pair fragment shown in large letters in the following DNA sequence. CTGTGAAAGGGTCATTTGACTCCCAAAAGGGTCACGACCCATTGAGAACAACTGATGTAGA

CAGTGTATGAACATTCT TGCTAGTACTTGTGTGGTTAATGAGTTTGCTTTGAGCCATTTGAA TAGCTGTATAATAACAC ACTGTATAGTCTTAATTGCTATGTTCGTGTCTCATGATACTGAATATTTTTGTGT GTTTACTTGCCAAGTGTATTTTT TAAAACTTGTTTTTTTGCCTATTTTCAAGTGAAAATTCATGTTACTGTCCTTTTCATTAAT AAATAGATGTACACTAA

Question

1 points

7

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Adenine is to DNA as lysine is to: Question

1 points

8

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Protein is to protease as nucleic acid is to: Question 9

2 points

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A 1.5% agarose gel was run in TBE buffer. The table shows the distance migrated by molecular weight markers and an unknown sample. Calculate the approximate size (in base pairs, to the nearest 100 base pairs) of the unknown samples. Size of marker (base pairs) Distance migrated (mm) 21,200 12 5,000 18 3,500 23 1,900 32 1,400 39 900 48 800 51 600 62 Unknown 1 21 Unknown 2 45

Question 10

1 points

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A laboratory procedure requires you to add a reagent to 7nM. You have a 1uM stock solution available. The final reaction volume is 100uL. What volume of the stock solution should you add?

0.7uL

1uL

2uL

7uL

Question 11

1 points

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A laboratory procedure requires you to add 25pmoles of a reagent. You have a stock solution of 1uM available. The final reaction volume is 100uL. What volume of the stock solution should you add?

0.25uL

1uL

2uL

25uL

Question 12

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SDS-PAGE is a technique which is used to

separat e protein from nucleic acids.

identify the charge on a protein.

separat e protein molecu les by size.

assay e nzyme activity .

Question 13

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Why is RNA more difficult to handle in the laboratory than DNA?

It is usually double strande d which makes it harder to copy.

It is less stable and more likely to be degrad ed by nuclea ses.

It contain s uracil which cannot be paired with anothe r base.

It is found in long molecu les

Question 14

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Primers for PCR are generally supplied as lyophilised powder (ie dried down under vacuum). You have received 75nmoles of a primer. You want to add 20pmoles to your reaction of final volume 20uL. Which of the following procedures would give you the right concentration?

Dissol ve the dried prime rs in 750 uL water and add 2uL to the reacti on.

Dissol ve the dried prime rs in 750uL water then make a 1 in 10 dilutio n and add 2uL to the reacti on.

Dissol ve the dried

Question 15

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DNA sequencing requires

Deoxy nucleot ides and Ribonu cleotid es

Deoxy nucleot ides and dideox ynucle otides

Ribonu cleotid es and dideox ynucle otides

Dideox ynucle otides and deoxyn ucleosi des

Question 16

1 points

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What is the catalytic activity of reverse transcriptase?

It copies riboso mal RNA in the nucleol us.

It makes an RNA copy of a DNA molecu le.

It convert s RNA primers to DNA during replicat ion.

It makes a DNA copy of an RNA molecu le.

Question 17

1 points

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How is 5-bromo-4-chloro-3-indoyl-beta-D-galactoside (X-gal) used?

It is an induc er of betagalact osidas e which is used to initiat e transc riptio n of the gene, resulti ng in a blue colou r.

It is an essent ial nutrie nt which produ ces blue E. coli c olonie s when grow n in o n agar plates .

Question 18

1 points

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Why is it important to include a promoter sequence when creating a reporter plasmid?

The promo ter is the D NA seque nce w here RNA polym erase binds to initiat e transc riptio n.

The promo ter is necess ary for mRN A atta chme nt to the riboso me prior to transl ation.

The promo ter is requir

Question 19

1 points

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You have discovered a new protein which appears to be an enzyme. What would you need to carry out an assay of this enzyme?

DNA, RNA, riboso mes.

amino acids, substrat e, detecti on system.

product , coloure d substrat e, cDNA clone.

source of enzyme , substrat e, way of measur ing product .

Question 20

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A plaque assay would be used to detect recombinant virus. True

False

Question 21

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A southern blot is used to detect specific RNA sequences. True

False

Question 22

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When using the expression of the lac gene to detect recombinant plasmids, colonies containing recombinant plasmids will be blue. True

False

Question 23

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E. coli cells which are competent are those which are blocked from taking up foreign DNA molecules. True

False

Question 24

1 points

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What is a restriction enzyme? Select all correct answers.

An enzyme which copies DNA

A protein which cuts DNA sequen ces at specific sites.

An endonu clease

A molecu le which restrict s access to DNA.

Question 25

1 points

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Select all correct answers. Taq DNA polymerase is:

an enzyme which copies DNA into RNA at high temper ature.

usually found in an organis m living at high temper ature.

unable to copy DNA at temper atures under 40C.

a protein which is stable at 95C.

Question 26

1 points

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Which of the following applications would require a cDNA library? Select all correct answers

Identif ying clones for exo ns from a gene of interest .

Exami ning DNA copies of gene s express ed in a specifi c tissue.

Studyi ng total genomi c DNA from an organis m.

Clonin g DNA

Question 27

2 points is an enzyme which forms phosphate in a strand

bonds to join a 3' OH to a 5'

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