• • • • 4 Calories/gram Made Up Of Amino

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Protein • • • •

4 calories/gram Made up of amino acids 20 different amino acids Amino: nitrogen

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein • 9 essential amino acids – Histidine – Threonine – Valine – Tryptophan – Isoleucine – Leucine – Lysine – Phenylalanine – Methionine © 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein • Proteins are chains of amino acids connected by peptide bonds – Dipeptides, tripeptides, polypeptides

• 20 aas make infinite different proteins • Proteins fold and coil

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

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© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein

Protein in the body

• Denaturing protein = unfolding – Heat – Agitation – Acid – Base – Alcohol

• Tissues – Muscle, skin, connective tissue (tendons, ligaments), organs, bone, hair, nails – Keratin – Collagen

• Growth and repair • Energy • Enzymes – Lipase, lactase, etc © 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein in the body

Fluid and electrolyte balance

• Hormones – Insulin, glucagon

• Blood clotting • Acid-base balance in blood • Fluid and electrolyte balance

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

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Protein in the body • Antibodies – Fight off bacteria and viruses

• Melanin • Hemoglobin

How the body makes proteins • DNA in nucleus of each cell provides blueprints for thousands of proteins • Cells put together amino acids in a specific sequence to make proteins (hormones, enzymes, collagen, etc.)

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

The Variety of Proteins

The Variety of Proteins

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

The Variety of Proteins

Problems in protein synthesis • Genetic diseases can cause problems in protein synthesis, for example • Sickle cell anemia – One amino acid is different in hemoglobin – Alters shape of red blood cell – Makes it unable to carry oxygen – Symptoms:

© 2006 Thomson-Wadsworth

• Abnormal blood clotting • Joint pain • Greater risk of infection

© 2006 Thomson-Wadsworth

3

Protein in food • DRI: – 10 to 35% of calories – 0.8 gm/kg (adults)

• Most Americans get more than enough – > 1 gm/kg

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein in food • Sources:

Sources of protein in US

– Meat – Poultry – Fish – Eggs – Milk, cheese – Soy – Legumes – Grains – Vegetables

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein in food

Protein in food

• Animal proteins – Generally better digested and absorbed

• Plant proteins – Generally less well digested and absorbed

• When you get enough protein, as most in the US do, these differences are not significant © 2006 Thomson-Wadsworth

• Complete protein – All essential amino acids – Animal proteins and soy

• Incomplete protein – Low in one or more essential aa – Plant proteins

© 2006 Thomson-Wadsworth

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Protein in food

Amino Acid Composition

• Limiting amino acids • Low in grains: – Isoleucine – Lysine

• Low in legumes: – Methionine – Tryptophan

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein in food

Protein digestion

• Complementary proteins – Traditional food combinations • Beans and rice • Beans and corn (tortillas) • Legumes and wheat (split pea soup with crackers, pasta with beans, peanut butter sandwich) • Small amounts of animal protein complements plant proteins (eg. Asian cuisines)

© 2006 Thomson-Wadsworth

• Stomach acid denatures protein – Pepsin enzyme begins to break it down

• Small intestine – Pancreatic enzymes (proteases) – Enzymes in villi – Absorbed as individual amino acids

© 2006 Thomson-Wadsworth

Nitrogen balance • Positive nitrogen balance – Body adding protein – Athletes, pregnancy, children

• Nitrogen equilibrium – Take in enough protein, excrete excess – Most adults

• Negative nitrogen balance – Losing protein, losing muscle – Starvation, anorexia, astronauts, surgery © 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

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Protein Malnutrition • PEM – protein energy malnutrition or PCM – protein calorie malnutrition • Most common in children in developing countries

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein Malnutrition

Protein Malnutrition

• Marasmus

• Marasmus

– Chronic inadequate calories and protein – ‘Skin and bones,’ little muscle – Heart weakened – Poor growth

– Affects brain development • Learning is impaired

– Poor temperature regulation – Lethargy – Malabsorption – Infection

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Protein Malnutrition

Protein Malnutrition

• Kwashiorkor

• Kwashiorkor

– Acute calorie and protein deprivation – Often in older child weaned for new baby – Muscle wasting, poor growth, affects brain development

© 2006 Thomson-Wadsworth

– Edema – Proteins and hormones that maintain fluid balance are diminished • Fluids leak out of the blood and accumulate in the belly and legs

– Enlarged belly due to fatty liver © 2006 Thomson-Wadsworth

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Protein Malnutrition • Marasmus and kwashiorkor often overlap • If caught in time, starvation can be reversed – Fluid balances are most critical • Electrolyte imbalances may lead to heart failure

Will extra protein build muscle? • Adding more gas to your car won’t make it go faster • Resistance exercise builds muscle • Some is good, more is not necessarily better

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Too much protein?

Vegetarianism

• Strains kidneys, which must dispose of excess nitrogen • Risk of dehydration • Animal protein increases calcium loss, weakens bones • Excess protein stored as fat

• Well-planned vegetarian diets are associated with: – Obesity ↓ – Heart disease ↓ – High blood pressure ↓ – Cancer ↓ – Life span ↑

© 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

Vegetarianism

Vegetarianism

• Vegetarian diets often contain more – Fruits, vegetables, whole grains – Fiber – Potassium – Phytochemicals

• And less – Total fat – Saturated fat

• Religious reasons – Hindu, Seventh Day Adventist, etc

• Ethical reasons – Not wanting to kill animals – Inhumane treatment of animals – Environment

• Health • Occasionally, eating disorders © 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

7

Vegetarianism • • • • • •

Concerns for vegetarians • Vegan children

Lacto-ovo Lacto Vegan Fruitarian Raw food Macrobiotic

– Too much fiber

• Vitamin B12 – Fermented soy foods, seaweed, supplement

• • • • • © 2006 Thomson-Wadsworth

Iron Zinc Calcium Vitamin D Omega-3 fats © 2006 Thomson-Wadsworth

© 2006 Thomson-Wadsworth

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