Microbiologist With Four Years Research Experience In Molecular Biology.

CURRICULUM VITAE

RAJESH KUMAR Krishna hostel-217, National Dairy Research Institute, Karnal-132001 (Haryana) Mobile & SMS: +919253086059 Email & Chat: [email protected] OBJECTIVE Looking for a permanent position in a Hi-tech environment that would allow me to utilize my skills for human wellness through scientific approaches. ACADEMICS 

Doctoral Degree – Dairy Microbiology (2005-2009)

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NATIONAL DAIRY RESEARXH INSTITUTE, KARNAL, INDIA Post Graduation – Microbiology (2003-2005)

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BUNDELKHAND UNIVERSITY, JHANSI Graduation – Biology (1996-1999) CCS UNIVERSITY, MERRUT

RESEARCH/WORK EXPERIENCE Organisation: Molecular Biology Unit, D.M. Division, NDRI, Karnal Designation: Ph.D Scholar Area of research: “Molecular cloning, sequence analysis and expression of bile salt hydrolase from a potential indigenous probiotic Lactobacillus of human origin” under the supervision of Dr. V.K. Batish . Publications: Research Article: “Molecular cloning and sequence analysis of bsh gene from L. plantarum strain of Indian origin” accepted with minor revisions in Food biotech journal (Taylor and Francis). Abstracts: 

“Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin” in International Conference on Microbial Biotechnology held at Punjab University, Chandigarh (March 03-04, 2009). Authors: Rajesh Kumar, Sunita Grover, A.K. Mohanty and V.K. Batish.

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“Molecular cloning of bile salt hydrolase gene from a probiotic L. plantarum of human origin” in 2nd International Conference on Trends in Cell and Molecular Biology at Jawaharlal Nehru university (JNU), Delhi (Jan 06-08, 2008). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish.

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“Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin” in International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007). Authors: Rajesh Kumar and V.K. Batish Acession nos: EU 477374; EU770692;EU784663;EU836149;EU822811;EU887663; FJ179486;FJ179487 ACG59259; ACF35257; ACF05268; ACF04740; ACA49878; ACF35782; ACG76118.  Dissertation Training ( March 2005 - June 2005) N.D.R.I KARNAL. India Project Title: “Optimization of ultrafiltered retentate for manufacturing of CHAKKA by defined strain starters” under the supervision of Dr. S.K. Tomar (Sr. Scientist).  Summer Training ( June 2004 – July 2004) MOHAN MEAKINS LTD, GHAZIABAD (U.P) DABUR INDIA LTD, SAHIBABAD (U.P) Responsibilities: Literature survey, Development, optimization and execution of experiments regarding molecular cloning, sequence submission, recombinant protein expression, purification and characterization. Maintenance and demonstration of laboratory and instruments. Data compilation and interpretation, Documentation of Standard Operating Protocols.

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TECHNICAL SKILLS 

Purification and characterization of recombinant and native proteins with Gel filtration, Affinity, Ionexchange, Hydrophobic interaction chromatography on FPLC system, Ultrafiltration, Rotophor (Isoelectric focussing), denaturing and nondenaturing PAGE. Localization and identification of proteins by Western blotting and Zymograms.  Cloning, characterization, sequence analysis and optimization of recombinant protein expression. PCRs (BSH/Species/Genus/Gradient/RAPD). Agarose gel electrophoresis.  Nucleotide and amino acid sequence submission to NCBI. Efficient use of BLAST, FASTA, ClustalW and Geneious Pro (4.6.2) for sequence analysis, characterization and Primer designing. Nucleic acids isolation and purification. Optimization of different Colorimetric Assays. Anaerobes culturing in MK3 Anaerobic Workstation.  Maintenance and Experimental handling (Survival bleeding, fecal analysis, Dissection etc), of wistar rats for hypocholesterolemic studies. Plasma HDL-, LDL-, VLDL-, Total Cholesterol and TG estimation.  Bacterial Microencapsulation, Maintenance, enumeration, preservation and propagation of bacteria from diverse nature of samples. Characterization of Lactobacillus strains for various probiotic attributes. Computer skills Operating systems: Windows Vista, XP, 2000 and 98. Software tools: Geneious Pro (4.6.4), DNA Star, Gencheck, Sequin, SYSTAT etc. MS office: MS Word, MS Excel, MS PowerPoint. (2000, 2003 & 2007). Others: Installation and Maintenance of software and Windows. Personal skills - Appreciated by peers and supervisors alike for pioneering attitude, leadership qualities, strong personal and communication skills - Fluent in written and spoken English, Hindi.

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SCHOLARLY ACTIVITY 

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Posters Presentation “Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin” in International Conference on Microbial Biotechnology held at Punjab University, Chandigarh (March 03-04, 2009). Authors: Rajesh Kumar, Sunita Grover, A.K. Mohanty and V.K. Batish. “Molecular cloning of bile salt hydrolase gene from a probiotic L. plantarum of human origin” in 2nd International Conference on Trends in Cell and Molecular Biology at Jawaharlal Nehru university (JNU), Delhi (Jan 06-08, 2008). Authors: Rajesh Kumar, Sunita Grover and V.K. Batish. “Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin” in International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007). Authors: Rajesh Kumar and V.K. Batish. Conferences Actively Participated in 45th AMI conference held at NDRI Karnal (Nov 23 – 25, 2004).

Awards - Institutional Senior Research Fellowship awarded during Ph.D programme (Aug 2005- Feb 2009) through Indian Council of Agricultural Research. - Awarded best (Ist) poster presentation on “Molecular cloning, sequence analysis and expression of bile salt hydrolase (bsh) gene from indigenous probiotic Lactobacillus plantarum strain of human origin” in International Conference on Microbial Biotechnology held at Punjab University, Chandigarh (March 0304, 2009). - Awarded second best poster presentation on “Bile Salt Hydrolase activity of some potential indigenous probiotic lactobacilli of human origin” in International Conference on Traditional Dairy Foods held at NDRI, Karnal (Nov 14-17, 2007). 

EXTRA CURRICULAR ACTIVITY  

Delivered presentation and Lectures for students of DNA/VPNR clubs sponsored by DBT, Govt of India. Actively Participated in Annual sports held at NDRI, Karnal (2007, 2008 & 2009).

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PERSONAL DETAIL -

Father’s Name: Date of birth: Place of birth: Nationality: Sex/Marital Status: Tel. No:

Shri Om Prakash April 14, 1980 Thora, G.B. Nagar (U.P) Indian Male/Single +915738270290

REFERENCES 

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Dr. V.K Batish, Ph.D ([email protected]) Head & Pr. Scientist, Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, India Dr. Sunita Grover, Ph.D, PDF ([email protected]) Pr. Scientist, Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, India Dr. A.K. Mohanty, Ph.D, PDF ([email protected]) Sr. Scientist, Animal Biotechnology Centre, Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, India

DECLARATION: I hereby declare that the information furnished and entries made in this resume, by me are true to the best of my knowledge and belief and nothing has been concealed/distorted.

Date: June 18, 2009 Place: Karnal

(RAJESH KUMAR)

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UPDATED PROFILE - Current Company/ Organization: National Dairy Research Institute, Karnal - Expected Salary (per annum): 3.0 lac to 4.0 lac Rs. (Flexible) - Date/Place of Birth: April 14, 1980/ Thora. - Joining Period: First week of August 2009. - Current Location: Karnal - Total Experience: 04 years research work during my doctoral degree programme. - Relocation Preference: Anywhere - Professional References (Senior & Peer) (Compalete Contact information): Mentioned in resume. 1.Core Competencies: i.Protein purification, characterization, Conducting in vivo trials ii.Molecular cloning, sequence analysis, characterization, iii.Bacterial isolation, preservation, anaerobic culturing, Microencapsulation 2.Reason(s) for Exploring: Specify primary reason..I had finished my doctoral degree and looking for suitable job. 3.Compensation: a)Current CTC: …1.3 Lakhs. i.Fixed: ……10.5K/month ii.Variable: …NA b)Expected Compensation(%): …3.0 -4.0 Lakhs…… 4.Notice Period (Days): …One month….. 5.Gender: ……Male….. 6.Current Location: ……Karnal…. 7.Preferred Location(s): ……Anywhere

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