Biosafety Ppt

Dr. Rajesh Kumar

Ph.D (Dairy Microbiology) Molecular Biology Unit Dairy Microbiology Division N.D.R.I [email protected]

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BIOSAFTEY TRAINING Good Laboratory Practice. Emergency procedures Biosecurity Biological Safety/GMOs‘‘ Wastes Chemical safety Transport of biological samples pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Links and Useful numbers

Good Laboratory Practice Strict Definition: “A quality system concerned with the organisational process and the conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, archived and reported.”

How does GLP relate to academic research? 1. Safety 2. Reproducibility 3. Reporting

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1) Biosafety – your responsibilities Those who work in the University/organization should be aware of the University’s Statement of biosafety Policy (http://www.dbtbiosafety.nic.in) Everyone must know the risks and potential hazards of the materials and equipment he/she is working with and so be alert to any dangers. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

You should also: 1. Have had a safety induction & training – check the local rules in all laboratories that you work in 2. Know who your Safety Officer is – seek advice, report any incidents 3. Consider your health status (e.g. allergies) & any vaccination requirements (e.g. Hepatitis B) 4. Complete risk assessments so that you know • how to use, store and dispose of all chemicals • how to deal with any spills or accidents • the safety precautions required and for some equipment, e.g. safety cabinets, how they work pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Risk Assessments Hazardous Chemicals Control of Substances Hazardous to Health Regulations 2002 (COSHH).

Biological Hazards – Genetic manipulation, handling pathogens, Crime & Security Act 2001

Radiochemicals – Contact the safety office for permission before use.

Experimental risk assessments – Combination of hazards associated with a technique or protocol. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Emergency Procedures What to do in case of an accident or incident ? Telephone numbers:

or

Experimental Hall Operators: 2525(24h/24h) Safety Group : 2403 pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

EMERGENCY PROCEDURES The safety signs to recognize

Emergency exits and evacuation routes Fire extinguishers Spill kits and First aid kits Emergency showers Eye-wash fountains Know your nearest first aiders pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

SAFETY ICONS

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Safety reminders

HAZARD ICONS

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Safety reminders

Hazard symbols Radioactive

Highly flammable

You must be able to recognise and explain the significance of the following hazard symbols.

Oxidizer

Harmful

Biohazard

Corrosive

Toxic

Look for them around the laboratory – all your chemicals should be labelled appropriately pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Personal protective equipment

But also be aware of hazards from your work possibly affecting others working around you – use a fume cupboard, warn others, restrict access to where you are working. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Safety cabinets Class I biosafety Protects the user from aerosol hazards

Fume cupboards Designed to exhaust fumes from the laboratory environment. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Class III biosafety User works through gloves. The material within the cabinet is protected by hepa-filtered air. The inside of the cabinet operates at a negative pressure.

Class II biosafety Protects both the user and the material within the cabinet pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Biological hazard Categories HAZARD GROUP 1- Agent is unlikely to cause human disease HAZARD GROUP 2- Agent can cause human disease and may be a hazard; unlikely to spread to the community; effective prophylaxis or effective treatment available. HAZARD GROUP 3- Agent can cause severe human disease and presents a serious hazard; may present a risk of spreading to the community; effective prophylaxis or treatment available. HAZARD GROUP 4- Agent causes severe human disease and is a serious hazard; likely to spread to the community; no effective prophylaxis or treatment available. pdfMachine - is a pdf writer that produces quality PDF files with ease!

All work with Group 3 pathogens Get yours now! must be with permission from the safety office “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Blood and human tissue Particular concern over the potential presence of infectious agents including Hepatitis B & HIV. It is recommended that any worker be successfully immunised against HepB before commencing work with human/primate samples.

Usual recommendations: Check your risk assessment & local rules before starting, Assess the HepB/HIV risk in samples, Work under biocontainment conditions (safety cabinet), Take care in disposal (e.g. double-bag & autoclave prior to disposal with clinical waste) pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Biological Safety Biological agents Any agent which may cause any infection, allergy, toxicity or can create any hazard to human health and/or environment. Micro-organisms such as Fungi (including yeasts), Bacterias, Viruses, Prions,

Parasites Genetically modified organisms (GMO’’s) Cell cultures such as Mammalian cell cultures (animal and human) Plant cell cultures pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Biological Safety Hazard groups (Since 1994)

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Adult healthy worker!!!

Some rules for classifying… The classification does not take account of other hazardous properties eg. toxic, oncogenic or allergenic properties. The categorisation is based on the infective hazard to healthy adult worker, it does not allow for any , additional risk to individual workers caused by:pre-existing disease the effects of medication compromised immunity pregnancy or breastfeeding

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Hazard group

Biological Agent

Disease

1

E. coli K12, Yeast Baculovirus Expression CHO cells

None

2

Pseudomonas aeruginosa

Urinary tract & respiratory system infections, dermatitis and variety of systemic infections

2

Adenovirus type 1-5, 7

Rhinitis, pharyngitis, conjunctivitis, cough…

2

Adenovirus type 40, 41

Nausea, vomiting, diarrhea, malaise

2

Eppstein Barr Virus

Nasopharyngeal carcinoma, Burkitt’s lymphoma

2

Vaccinia Virus

Eczema, vesicular and pustular lesion

3

HVB, HBC, HIV, Bacillus anthracis

Hepatitis B, Hepatitis C, AIDS, Anthrax/Blackleg

4

Marburg Virus

Hemorrhageic fever

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Genetically modified organisms Genetic Modification is defined as the altering of the genetic material of an organism by a way that does not occur naturally by mating and/or natural recombination. It is the policy of the University that all work involving GMOs is carried out in such a way as to prevent undue risk either to those carrying out the work, to those who may otherwise be affected by the work, or to the environment. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Genetically modified organisms Risk assessments for new projects must be completed and submitted to a GM Safety Committee • hazard identification • estimation of the severity of the consequence • determination of a provisional containment class • environmental considerations • estimation of likelihood of harm occurring • can all hazards be properly controlled

Results in assignment of the final containment and activity classification 1-3 (classes 2 & 3 notifiable to HSE) pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Genetically modified organisms •Work must be carried out in a suitably equipped and designated containment laboratory •Periodic reviews of risk assessments done •Health surveillance may be required •Records of exposure may be required •Emergency plans are needed

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Before starting a project…. Involving GMO’s… Every project should be registered in the existing license for contained use of genetically modified organisms (CGG) The project may also be registered and approved by the safety officer

Involving Pathogens and human cell lines…  Work in a special designed laboratory  Project registered and approved  Specific training in using the laboratory and handling cell lines or pathogens  Check on medical follow follow-up (vaccinations if possible or necessary) pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

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Waste disposal Waste must be disposed of correctly: autoclave bags or the yellow carton bin dedicated for incineration Stainless steel container Sharp bin containers Treatment of liquid waste with disinfectant

STAFF SHOULD NEVER BE ENDANGERED BY DISPOSING OF WASTE INAPPROPRIATELY pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

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Biohazardous waste must be rendered safe before disposal

Clinical waste

Disinfection Sharps

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Autoclave

Non-infectious biological waste Liquid biological waste containing GMO’s must be inactived by autoclaving before it can be poured down into a plastic container for chemical waste treatment. (Gelyfication of liquids in the pipeline) ATTENTION: Solutions containing SeMet, corrosives or any other toxic compound must be inactivated for biological contaminant first, and treated afterwards as chemical waste. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

Chemical risks How do I protect myself and the environment ?

Protocol well known. Chemicals and material safety data sheet and (MSDS) in sample lab or on the lab web read. Use of small quantities of chemicals; quantities consider safer substitute protocols when possible. Wear safety glasses, proper gloves, and a lab coat when handling chemicals Disposal of of chemical waste appropriate. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

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Storage of chemical products No more chemicals than needed should be stored. Always read the chemical's label (and MSDS). Mark it with the date of receipt/opening before storage. Never store a chemical with an obscured or missing label. Define separate storage areas for highly toxic chemicals. Never store liquid hazardous chemicals above eye level. pdfMachine - is a pdf writer that produces quality PDF files with ease! Get yours now! “Thank you very much! I can use Acrobat Distiller or the Acrobat PDFWriter but I consider your product a lot easier to use and much preferable to Adobe's" A.Sarras - USA

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