Iso Grid Ecoli
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Food Safety Online Ordering
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ For Enumeration of Total E. coli and E. coli O157:H7 Durable ISO-GRID System
Disposable NEO-GRID System
Results
SD-39 Agar Intended Use Using SD-39 Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for enumeration of E. coli and E. coli O157:H7. The Test The ISO-GRID and NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. A sample’s total E. coli and E. coli O157:H7 is enumerated through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on SD-39 Agar. SD-39 Agar is specifically formulated to enumerate a sample’s total E. coli and E. coli O157:H7. After incubation the membrane filter is examined, and all squares containing one or more pink and/or green colonies are counted. The total number of positive squares is converted to the corresponding most probable number (MPN) using one of the methods described in the ISO-GRID Methods Manual, and the total E. coli MPN per gram is calculated. A presumptive E. coli O157:H7 MPN per gram is calculated by counting all the squares containing one or more pink colonies and following the same procedure. The presence of E. coli O157:H7 can be confirmed using the confirmation procedures described in the Methods Manual. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate in a specified diluent. Filter 1 mL of the homogenate through the ISO-GRID or NEO-GRID membrane filter. Place the membrane filter on the surface of SD-39 Agar. Incubate inverted plate for 24 hours at 44.5°C.
Refer to the Methods Manual for complete instructions, and other available test methods. Product Specifications Approvals:
38
AOAC Official Method #997.11
Proteose Peptone SM47 Yeast Extract Sodium Chloride L-Lysine Monohydrochloride Dextrose Sorbitol Magnesium Sulfate 7-Hydrate Monensin Sodium Glucuronate Novobiocin Phenol Red X-glucose Agar Final pH: 7.2 ± 0.2
5 g/L 3 g/L 5 g/L 10 g/L 2.5 g/L 20 g/L 1.5 g/L 0.038 g/L 0.5 g/L 0.0075 g/L 0.12 g/L 0.05 g/L 15 g/L
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6907 SD-39 Agar 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
Foodborne Bacteria
www.neogen.com/foodsafetyorder.htm
NEO-GRID™ / ISO-GRID™ For Enumeration of Total E. coli and E. coli O157:H7 Durable ISO-GRID System
Disposable NEO-GRID System
Results
SD-39 Agar Intended Use Using SD-39 Agar, the ISO-GRID and NEO-GRID Membrane Filter Systems are used to provide a method for enumeration of E. coli and E. coli O157:H7. The Test The ISO-GRID and NEO-GRID systems are based on the principle of hydrophobic grid membrane filtration. A sample’s total E. coli and E. coli O157:H7 is enumerated through the use of a unique membrane filter containing a grid of 1,600 squares. First, a diluted sample is prefiltered to eliminate any food particles present. The sample is then filtered through a hydrophobic membrane, and the membrane is placed on SD-39 Agar. SD-39 Agar is specifically formulated to enumerate a sample’s total E. coli and E. coli O157:H7. After incubation the membrane filter is examined, and all squares containing one or more pink and/or green colonies are counted. The total number of positive squares is converted to the corresponding most probable number (MPN) using one of the methods described in the ISO-GRID Methods Manual, and the total E. coli MPN per gram is calculated. A presumptive E. coli O157:H7 MPN per gram is calculated by counting all the squares containing one or more pink colonies and following the same procedure. The presence of E. coli O157:H7 can be confirmed using the confirmation procedures described in the Methods Manual. Test Procedure 1. 2. 3. 4.
Prepare a sample homogenate in a specified diluent. Filter 1 mL of the homogenate through the ISO-GRID or NEO-GRID membrane filter. Place the membrane filter on the surface of SD-39 Agar. Incubate inverted plate for 24 hours at 44.5°C.
Refer to the Methods Manual for complete instructions, and other available test methods. Product Specifications Approvals:
38
AOAC Official Method #997.11
Proteose Peptone SM47 Yeast Extract Sodium Chloride L-Lysine Monohydrochloride Dextrose Sorbitol Magnesium Sulfate 7-Hydrate Monensin Sodium Glucuronate Novobiocin Phenol Red X-glucose Agar Final pH: 7.2 ± 0.2
5 g/L 3 g/L 5 g/L 10 g/L 2.5 g/L 20 g/L 1.5 g/L 0.038 g/L 0.5 g/L 0.0075 g/L 0.12 g/L 0.05 g/L 15 g/L
Ordering Information Prod.# Product description For Use With Either the NEO-GRID or ISO-GRID Test System 6907 SD-39 Agar 6810 1-Place Filtration Manifold 6800 3-Place Filtration Manifold 6801 6-Place Filtration Manifold 6821 Rubber Tubing (10 feet) 6822 Side-Arm Flask Assembly (1 L) 6824 Vacuum Pump - ¼ hp (Gast) 6828 Forceps For Use With The NEO-GRID Test System Only 6848 NEO-GRID Test System - Contains 48 individually packaged membrane filtration systems 6825 NEO-GRID Silicone Stopper 6826 Filta Tips (box of 100) 6827 Filter Bags - 24 oz Whirl-Pak (box of 250) 6829 1.0 mL Sterile Serological Pipettes (bag of 50) For Use With The ISO-GRID Test System Only Please see page 95 for the required ISO-GRID equipment and accessories.
Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com
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