Group 6-jade Ip Proposal

Efficiency of Crab Shells and Acacia Seeds in Purifying Water PROBLEM: Are crab shells and acacia seeds effective in purifying water? HYPOTHESIS: We think that if we utilize crab shells and acacia seeds in unpurified water then it will neither have a significant effect on this liquid nor it will purify unpurified water. EXPERIMENTAL DESIGN: a) Controlled Variable - a sample of unpurified water from Iloilo River b) Manipulated Variable(s) - another sample of unpurified water from Iloilo River - Acacia seeds - Crab shells b) Responding Variable - impurities in water

MATERIALS: ITEMS Crab Shells Acacia Seeds Sterilized bottle Erlenmeyer Flask Flashlight Mortar and Pestle NaOH solution Distilled Water Commercial Faucet Filtering Device Glass bottle Test tube pH paper Beaker Phenolphthalein

QUANTITY 400 grams 40 grams 2 units 6 units 1 unit 1 unit 400 mL. 2 liters 1 unit 2 units 2 units 2 units 1 unit 10 grams

PROCEDURE: a) COLLECTING PROCESS Water samples are to be collected from Iloilo River passage near Gaisano Mall. The bottle should be held in the lower part and was submerged at a depth of about 25 centimeters with the bottle mouth slightly facing upwards. Remember that if there is a current, that bottle mouth should face towards the current. The 1st water sample should be marked ‘A’ because this should be our controlled sample. The 2nd water sample should be marked ‘B’ and is to be used for the investigation. Acacia seeds are to be collected either from West Visayas State University, Iloilo National High School or St. Clements church. The rest of the materials are expected to be purchased. b) MAIN PROCESS Acacia seeds are to be broken into two, collecting the yellowish, sticky part and leave to dry. Crab shells are to be pounded and dried. The shells were supposed to be immersed in 200 mL. of 0.02n NaOH solution to solidify the chitosan gel. After this procedure, the

membrane should be washed with distilled water to remove the NaOH solution. The membrane should be air dried after this. A Ca membrane is to be needed in this treatment. The Ca membrane is made out of a thin film of 1% solution of chitosan in 2% acetic acid and cast it to a cellulose acetate (CA) micro filtration membrane. A commercial faucet filtering device is to be used. Acacia seeds and chitosan UF membrane are to replace stones inside the filtering device if there are any. Water sample ‘B’ is then to be filtered by the commercial faucet filtering device.