Enzymes Models Of Action

  • Uploaded by: Brandon Hunter
  • 0
  • 0
  • June 2020
  • PDF

This document was uploaded by user and they confirmed that they have the permission to share it. If you are author or own the copyright of this book, please report to us by using this DMCA report form. Report DMCA


Overview

Download & View Enzymes Models Of Action as PDF for free.

More details

  • Words: 411
  • Pages: 29
18th October

Enzyme Action Learning objective: to describe different models for enzyme action and to investigate factors which control the rate of an enzyme reaction

Lock and Key

Lock and Key However certain substances can bind to the enzyme at sites other than the Active site and modify its activity (inhibitors/co-factors) Idea that the enzyme is flexible

Induced Fit

Enzyme reactions

enzyme + substrate

enzyme-substrate complex

Enzyme reactions

enzyme + substrate E +S

enzyme-substrate complex ES

Enzyme reactions enzyme + substrate

enzyme-substrate complex

E +S

ES

enzyme-substrate complex ES

enzyme + product E +P

Enzyme activity How fast an enzyme is working Rate of Reaction

Enzyme activity How fast an enzyme is working Rate of Reaction Rate of Reaction = Amount of substrate changed (or amount product formed) in a given period of time.

Rate of Reaction

Enzyme activity

Variable you are looking at

Enzyme activity

Four Variables

Enzyme activity Temperature Four Variables

pH Enzyme Concentration Substrate Concentration

Rate of Reaction

Temperature

Rate of Reaction

Temperature

0

10

20

30

40

50

60

Temperature

5- 40oC Increase in Activity

Rate of Reaction

40oC - denatures

0 <5oC - inactive

10

20

30

40

50

60

Effect of heat on enzyme activty If you heat the protein above its optimal temperature bonds break meaning the protein loses it secondary and tertiary structure

Effect of heat on enzyme activty

Denaturing the protein

Effect of heat on enzyme activty

Denaturing the protein ACTIVE SITE CHANGES SHAPE SO SUBSTRATE NO LONGER FITS Even if temperature lowered – enzyme

can’t regain its correct shape

Rate of Reaction

pH

Rate of Reaction

pH

1 2 3 4 5 6 7 8 9

pH

Rate of Reaction

Narrow pH optima

1

2

3

4

5

6

7

8

9

pH

Rate of Reaction

Narrow pH optima

WHY?

1

2

3

4

5

6

7

8

9

pH

Rate of Reaction

Narrow pH optima

Disrupt Ionic bonds - Structure Effect charged residues at active site

1

2

3

4

5

6

7

8

9

Rate of Reaction

Enzyme Concentration

Rate of Reaction

Enzyme Concentration

Enzyme Concentration

Rate of Reaction

Substrate Concentration

Rate of Reaction

Substrate Concentration

Substrate Concentration

Substrate Concentration

Rate of Reaction

Active sites full- maximum turnover

Substrate Concentration

This powerpoint was kindly donated to www.worldofteaching.com

http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.

Related Documents

Enzymes In Action
July 2020 0
Enzymes
November 2019 31
Enzymes
June 2020 13
Enzymes
October 2019 25
Enzymes
May 2020 15

More Documents from ""