Enzymes

  • November 2019
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Amino Acids, Proteins, and Enzymes Enzymes Enzyme Action Factors Affecting Enzyme Action Enzyme Inhibition

1

Enzymes • • • • • • •

Catalysts for biological reactions Most are proteins Lower the activation energy Increase the rate of reaction Activity lost if denatured May be simple proteins May contain cofactors such as metal ions or organic (vitamins) 2

Name of Enzymes • End in –ase • Identifies a reacting substance sucrase – reacts sucrose lipase - reacts lipid • Describes function of enzyme oxidase – catalyzes oxidation hydrolase – catalyzes hydrolysis • Common names of digestion enzymes still use –in pepsin, trypsin

3

Classification of Enzymes Class • Oxidoreductoases • Transferases • Hydrolases • Lyases • Isomerases • Ligases

Reactions catalyzed oxidation-reduction transfer group of atoms hydrolysis add/remove atoms to/from a double bond rearrange atoms combine molecules using ATP 4

Examples of Classification of Enzymes • Oxidoreductoases oxidases - oxidize ,reductases – reduce • Transferases transaminases – transfer amino groups kinases – transfer phosphate groups • Hydrolases proteases - hydrolyze peptide bonds lipases – hydrolyze lipid ester bonds • Lyases carboxylases – add CO2 hydrolases – add H2O

5

Learning Check E1 Match the type of reaction with the enzymes: (1) aminase (2) dehydrogenase (3) Isomerase (4) synthetase • • • •

Converts a cis-fatty acid to trans. Removes 2 H atoms to form double bond Combine two molecules using ATP Adds NH3 6

Solution E1 Match the type of reaction with the enzymes: (1) aminase (2) dehydrogenase (3) Isomerase (4) synthetase • 3 Converts a cis-fatty acid to trans. • 2 Removes 2 H atoms to form double bond • 4 Combine two molecules using ATP 1 Adds NH3 • 7

Enzyme Action: Lock and Key Model • An enzyme binds a substrate in a region called the active site • Only certain substrates can fit the active site • Amino acid R groups in the active site help substrate bind • Enzyme-substrate complex forms • Substrate reacts to form product • Product is released 8

Lock and Key Model

P S

+

+

S

P

E

+

S

ES complex

E

+

P 9

Enzyme Action: Induced Fit Model • Enzyme structure flexible, not rigid • Enzyme and active site adjust shape to bind substrate • Increases range of substrate specificity • Shape changes also improve catalysis during reaction

10

Enzyme Action: Induced Fit Model

P S

S P

E

+

S

ES complex

E

+

P 11

Learning Check E2 A. The active site is (1) the enzyme (2) a section of the enzyme (3) the substrate B. In the induced fit model, the shape of the enzyme when substrate binds (1) Stays the same (2) adapts to the shape of the substrate 12

Solution E2 A. The active site is (2) a section of the enzyme B. In the induced fit model, the shape of the enzyme when substrate binds (2) adapts to the shape of the substrate

13

Factors Affecting Enzyme Action: Temperature • Little activity at low temperature • Rate increases with temperature • Most active at optimum temperatures (usually 37°C in humans) • Activity lost with denaturation at high temperatures

14

Factors Affecting Enzyme Action Optimum temperature

Reaction Rate

Low High Temperature 15

Factors Affecting Enzyme Action: Substrate Concentration • Increasing substrate concentration increases the rate of reaction (enzyme concentration is constant) • Maximum activity reached when all of enzyme combines with substrate

16

Factors Affecting Enzyme Action Maximum activity

Reaction Rate

substrate concentration 17

Factors Affecting Enzyme Action: pH • Maximum activity at optimum pH • R groups of amino acids have proper charge • Tertiary structure of enzyme is correct • Narrow range of activity • Most lose activity in low or high pH

18

Factors Affecting Enzyme Action

Reaction Rate Optimum pH

3

5

7

9

11

pH 19

Learning Check E3 Sucrase has an optimum temperature of 37°C and an optimum pH of 6.2. Determine the effect of the following on its rate of reaction (1) no change (2) increase (3) decrease A. Increasing the concentration of sucrose B. Changing the pH to 4 C. Running the reaction at 70°C

20

Solution E3 Sucrase has an optimum temperature of 37°C and an optimum pH of 6.2. Determine the effect of the following on its rate of reaction (1) no change (2) increase (3) decrease A. 2, 1 Increasing the concentration of sucrose B. 3 Changing the pH to 4 C. 3 Running the reaction at 70°C 21

Enzyme Inhibition Inhibitors • cause a loss of catalytic activity • Change the protein structure of an enzyme • May be competitive or noncompetitive • Some effects are irreversible

22

Competitive Inhibition A competitive inhibitor • Has a structure similar to substrate • Occupies active site • Competes with substrate for active site • Has effect reversed by increasing substrate concentration 23

Noncompetitive Inhibition A noncompetitive inhibitor • Does not have a structure like substrate • Binds to the enzyme but not active site • Changes the shape of enzyme and active site • Substrate cannot fit altered active site • No reaction occurs • Effect is not reversed by adding substrate 24

Learning Check E4 Identify each statement as describing an inhibitor that is (1) Competitive (2) Noncompetitive A. B. F. D.

Increasing substrate reverses inhibition Binds to enzyme, not active site Structure is similar to substrate Inhibition is not reversed with substrate 25

Solution E4 Identify each statement as describing an inhibitor that is (1) Competitive (2) Noncompetitive A. B. C. D.

1 2 1 2

Increasing substrate reverses inhibition Binds to enzyme, not active site Structure is similar to substrate Inhibition is not reversed with substrate 26

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