Department Of Ayurved Research And Development

Department of Ayurved Research and Development Past Research: In respect of various diseases, which are considered incurable in the modern medical system, the ancient sages had prescribed successful treatment. In line with the treatment of those sages, deep study of the classics and with the grace of God, works related to upliftment, development and research in the field of Ayurved, have been undertaken with full dedication by the Brahmakalpa Chikitsalaya governed by Divya Yog Mandir Trust (SIROs). Ayurvedic treatment and research work is being carried on under the supervision of Acharya Balkrishna. In the Brahmakalpa Chikitsalaya various open level clinical research projects were undertaken to evaluate the beneficial effects of combination of classical and proprietary ayurvedic drugs along with development and improvement of new scientifically evaluated drugs i.e.; [1] Research project on ‘Formulation and process development of proprietary ayurvedic medicine for various ailments as compared to existing therapies.’ [2] Clinical evaluation of subjects suffering with ailments of digestive system. [3] Clinical evaluation of subjects suffering with ailments of hepato-biliary system. [4] Clinical evaluation of anti-diabetic combination suffering with Non-Insulin Dependent Diabetes Mellitus (NIDDM). [5] Clinical evaluation of subjects suffering with disorders of cardiovascular system. [6] Clinical evaluation of subjects suffering with Rheumatoid Arthritis (RA). [7] Clinical evaluation of subjects suffering with Chronic Renal Failure (CRF). [8] Clinical Study on “An ayurvedic formulation use on asthmatics patients". [9] Clinical Study on “An ayurvedic formulation use on Muscular Dystrophy (DMD) Patients". [10] Effect of Mukta Vati on patients of Hypertention (HTN). [11] Clinical evaluation of paralysis patients. [12] Effect of an ayurvedic combination on patients of migraine (Ardhavbhedak). [13] Effect of an ayurvedic combination on patients of epilepsy (Apasmar). Outcomes of there research activities were published in previous issues of monthly research journal/ magazine ‘Yog Sandesh’

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ABOUT DEPARTMENT Aims: To enhance the capability of the DYM(T), PYP(T), PAL and PFHPL as a premier institution for research in Ayurved and forge strategic alliance with similar establishments and constantly strive for excellence in basic and applied knowledge for efficient understanding of the cause and prevention of human diseases and their management. Objectives: 1. Scientific exploration of mysteries hidden in ancient Indian texts. 2. Extensive Survey and Research on Medicinal flora of Himalayas and other potential areas. 3. Novel Chemical compound Research Program, Pharmacological Research Program including Toxicological studies, Drug Standardization Research Program, Clinical Research Program etc. 4. To work in the field of Molecular markers development of Herbo-mineral material 5. Detection of adulterants/substitution material by their sequence characterized amplification methods. 6. Markers assisted selection of desirable chemotypes including Plant Tissue Culture. 7. Expanding horizons in food technology and processing for industrialization of Agriculture in India. 8. Formulate aims and patterns of research on scientific lines in ayurvedic systems of medicine. 9. Initiate, develop, encourage and coordinate scientific research in fundamental and applied aspects of ayurved. 10. To study of diseases, their prevention and cure, especially with emphasis for covering the rural population of the country. 11. Establish a state-of-the-art research facility dedicated to ayurvedic research i.e. establishment of centre of excellence for ayurved research 12. To Fulfill the imagination of HH Swami Ramdev ji and HH Acharya Balkrishna ji to make disease free India and World by reestablishing traditional Indian knowledge.

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AYURVED RESEARCH TEAM 1. Acharya Balkrishna Chairman, Research & Development 2. Avnish K. Upadhyay, B.Sc, B.A.M.S., P.G.D.C.R., Ph.D. (pur.) Senior Scientist and Head 3. Subhas C. Nayak, B.A.M.S. Senior Research Fellow 4. Kuldeep Kataria, B.A.M.S. Research Associate 5. Sangeeta Maharathi, B.A.M.S. Research Associate 6. Samhita Sen, M.Sc., P.G.D.N.R.M. (FRI, Dehradun) Research Associate 7. Preetam Singh, M.Sc. (Geology) Research Associate Research Team of Associated Organizations: 1. Arvind Kumar, M.Sc., Ph.D. (Biotechnology) Senior Scientist and Head, R&D, PAL 2. Anupa Tiwari, M.Sc(Inorganic Chemistry), Ph.D.(pur.) Senior Scientist 3. N.M. Sahu, B.A.M.S., P.G.D.P.P.M. (IPER, Pune) Formulation Scientist 4. B.D. Ohdar, M.Sc.(Botany), P.G.D.N.W.F.P. (FRI, Dehradun) Research Associate RESEARCH ADVISORY BOARD 1. Dr. P.L. Gautam

Vice Chancellor, G.B. Pant University

2. Dr. B.D. Sharma

Ex. Principal of NBPGR

3. Mr. Shrikant Chandola

Chief Conservator of Forests and Wild Life

4. Dr. Akhila Anand

Senior Assistant Director, CIMAP

5. Dr. Pradeep Kumar

Principal, RAC, Haridwar

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6. Prof. S.D. Upadhyay

HOD, Deptt. of Kaya Chikitsa, RAC

7. Dr. Kaushal Kumar

Senior Scientist, Patanjali Herbal Garden

INSTITUTIONAL ETHICS COMMITTEE (IEC) 1. Chairman [Internal]

Acharya Balkrishna

2. Member Secretary

Sri. Lalit Mohan

3. Member

Avnish K. Upadhyay

4. Member

Prof. S.D. Upadhyay

5. Member

Sri Mahant Rajendra Das Kothari

6. Member

Dr. Vinod Upadhyay

7. Member

Dr. P.L.Sharma

8. Member

Dr. Satish Chandra

9. Member

Sri Uttam Singh Chowhan

RECOGNITION(S) 1. Recognised as Scientific and Industrial Organisations (SIROs), by the Department of Scientific & Industrial Research, Ministry of Science & Technology, Govt. of India -Vide Lett. No. 14/408/2005- TU-V, Dt. 20 July, 2006. 2. Accreditation as Research & Development organization by Central council for Research in Ayurveda and Siddha (CCRAS), Deptt. of AYUSH, Ministry of Health & Family Welfare, Govt. of India [Under Process] RESEARCH PROJECT(S) Completed: 1. Formulation development and clinical evaluation of various herbo-mineral products. 2. Process development of various classical ayurvedic preparations described in ancient texts. 3. Myths and scientific contribution to explore the Ashtavarg Plants in the Himalayas. 4. Demographic study on “Role of Divya Yog Mandir Trust for the scientific development of Yog, Pranayam and Indian System of Medicine”.

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5. Antimicrobial and bronchodilatory effects of some newly introduced medicinal herbs as hawan samagri in Vedic Agnihotra. 6. Physio-chemical analysis of ingredients in Divya Madhunashini and its Pathophysiological effects on Diabetes Mellitus with and without SRY. 7. Chemical and Biological investigations related to process development of some metallic preparations used in Indian System of Medicine. 8. Process Development and commercialization of Extraction technology of herbals. 9. A novel method and scheme for Standardization of Divya Madhunashini: A Herbal Medicine 10. Project for development of “An extraction process for Emblica officinalis Gaertn. 11. A noval method of detection and differenciation of giloy satwa (Tinospora cordifolia) from coomen adulterent such as potteto and maize satwa. 12. In-vivo toxicological study of Drishti eye drop. 13. In-vivo toxicological study on plant Ricinus communis Linn. 14. Efficacy evaluation of Drishti Eye drop (A product of Patanjali Ayurved Limited) in different eye disorders. 15. Effect of Ashwashila Capsule (An Ayurvedic Preparation of Patanjali Ayurved Limited) In Patients with Non-Insulin Dependent Diabetes Mellitus [NIDDM]. 16. “Detection, Identification and Differentiation of urine of cow of Indian breed amongst the urines of other breed cow urine, goat and buffalo urine.” 17. A novel field test kit which enables spot test for the detection and identification of Raw Herbs Active constituent in the field within a few minutes. 18. Isolation, Identification and characterization of specific chemical compound marker of 1000.8 KD in shilajit sample responsible for the therapeutic activity of shilajit prescribed in ayurved as panacea. 19. The novel aroma composition of more then 50 compounds in specific ratio isolated from Sanjeevni plant ‘Saussurea gossypiphora’ for the prevention and treatment of psychological disorders.

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Ongoing: 1. Scientific evaluation and validation of lesser known herbs potentially used as folk medicine by Jaunsari tribe and its Synergy with Ayurveda. 2. Effects of Mukta Vati (A Nobel Herbomineral Preparation of Divya Pharmacy) in Patients with High-Normal to Stage I Hypertension: A randomized controlled comparative study 3. Effect of Madhunashini (A Polyherbal preparation by Divya Pharmacy) on Diabetic Profile: A randomized controlled comparative study 4. Identification and authentication of medicinal plants “Saussurea gossypiphora” by various biotechnological tools. 5. ‘Isolation of markers (Chemical, Molecular and Biochemical Metabolites) for the authentication of some most common medicinal plants’. 6. To study the peak plasma concentration of metals after the consumption of bhasma. 7. Open prospective clinical trial and study of shilajit as potential anti aging agent that maintained telomere length in cells by increasing the activity of telomerase, the enzyme involved in telomere synthesis. 8. A Randomized, Double-Blind, and Controlled Study of a Herbomineral Preparation of Divya Pharmacy in Adult Patients of mild to moderate Bronchial Asthma. 9. Development of in-vitro facility for biological activity testing of bioactive constituents of medicinal and aromatic plants using hella, Cancer cell lines. 10. Project for Collection of 1000 germplasm from different sources to develop a live bank of variants of medicinal and aromatic plants. 11. Development the deployment of rapid and suitable analytical procedures for testing and evaluation of medicinal and aromatic plant materials. 12. Development of immunoactive peptides as Immuno-modulator. 13. Isolation, characterization and development of non synthetic salicylic acid as antipyretic drug from the various vegetable sources. 14. New Markers development of most frequent herbs and to attain the patent of said markers.

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RESEARCH PUBLICATION (S) 1. Acharya Balkrishna, Upadhyay A.K., Yadav A.; “Importance of Prayanama in modern life, A Scientific Review” Ayush Tomorrow,2005;1(1): 1-20 2. Balkrishna, Acharya; Reports of Clinical trials on various disorders in Yog Sandesh a monthly research magazine published by Divya Prakasan, Haridwar 3. Acharya Balkrishna, Upadhyay A.K., Yadav A, Pant J. “An introduction of Diabetes & Physio Chemical Analysis of Active ingredients in Madhunanshini Vati” Ayush Tomorrow,2005;1(2): 25-29 4. Acharya Balkrishna, Upadhyay A.K., Ruchita T.Upadhyay “Effect of Pranayama [Voluntary Regulated Yoga Breathing] and Yogasana [Yoga Postures] in Diabetes Mellitus (DM): A Scientific Review” Journal of Complementary and Integrative Medicine, 2008. 5. Acharya Balkrishna, Upadhyay A.K.; “Assessment of favorable and unfavorable responses from Indian System of Medicine (ISM) Professionals towards ISM as a Total Health System” Indian Journal of Psychiatry, 2008 [Under Review] 6. Avnish K. Upadhyay, Arvind Kumar, An integrated approach to combat cancer (neoplasm): in perspective of Ayurveda, Ind Jour. Ancient Scie. & Yoga, 2009, 2(1): 7. Avnish K. Upadhyay, Kaushal Kumar, Effects of combination of Shilajit (Asphaltum punjabianum) and Ashwagandha (Withania somnifera) on fasting blood sugar and lipid profile, Journal of Pharmacy Research, 2009. 8. Avnish K. Upadhyay, Kaushal Kumar, Arvind Kumar, Effect of Ashwashila Capsule (An Ayurvedic Preparation of Patanjali Ayurved Limited) In Patients with NonInsulin Dependent Diabetes Mellitus [NIDDM], Complimentary therapies in Medicine, 2009 (Communicated). 9. Avnish K. Upadhyay, Kaushal Kumar, Arvind Kumar, Studies on Tinospora cordifolia (Willd.) Hook. f. & Thoms. (Guduchi): in synergy with human ailments, Int J Ayurveda Research, 2009. (Communicated). 10. Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K 2008, In-vivo toxicological study of Drishti eye drop, Progressive Agriculture (Accepted).

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11. Kumar, A; Gaurav, S.S; Sirohi S.PS, Acharya, B.K and Sharma, S. 2008, Detection and identification of alkaloid of RICINUS COMMUNIS LINN by newly develop Thin Layer Electro chromatographic method, Progressive research (Accepted). 12. Kumar, A; Gaurav, S.S; Sirohi, S.PS and Acharya, B.K 2008. In-vivo toxicological study of extract of plant Ricinus Communis Linn.for development of plant extract as safe ,accurate & effective new anticancer and anti HIV agent, Progressive Agriculture (Accepted). 13. Kumar, A; Gaurav, S.S; Sirohi S.P.S and Acharya, B.K 2008, Determination of protein toxins markers of plant, RICINUS COMMUNIS LINN by new complimentary technique, Indian Journal of Forensic Science(Communicated) 14. Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K 2008, A rapid and simple scheme for the standardization of polyherbal drugs, International Journal of Green Pharmacy (In-Press). 15. Kumar, A; Gaurav, S.S; Sirohi S.PS and Acharya, B.K. 2009. Molecular characterization of Ashwagandha Indian ginseng (Withania somnifera) Progressive Agriculture (In-Press) 16. Kumar, A, 2009, Volatile phytochamical analysis from Roots of Withania somnifera, E-JOURNAL OF CHEMISTRY (Accepted). 17. A.K Indrayan, Sudeep Sharma, Arvind Kumar 2008, Study of fatty acid composition of seed oil of nelumbo nucifera Gaetrn. & its uses, Journal of Lipid Science & Technology (Accepted). 18. Kumar A, 2008, Yog Paranayam and Oxygen: Conception & Facts, Published: 2008-04-05, Articles Gratuits (Exercise & Fitness U.K.) 19. Kumar, A; Gaurav, S.S; and Acharya, B.K 2008, To study the effect of practicing of novel breathes with specific posture mechanism on maintaining the airway hygiene by expelling the harmful organisum. International Journal of YOGA (Under revision). 20. Kumar,A, Gaurav, S.S; and Acharya, B.K.2009. Detection of oxidation preventive proteins in the practicners of Pranayama. Journal of Pharmacy Research.2:268-72 21. Kumar, A and Acharya, B.K 2008,To study the effect of the sequence of seven pranayama by Swami Ramdev on gene expression in

Department of Ayurved Research and Development

leukemia

patients and rapid

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interpretation of gene expression, Journal of Clinical Pathology (British Medical Journal). (Accepted) 22. Kumar, A; Gaurav, S.S; Sirohi, S.P.S, Acharya, B. K, Ohdar, B and Tiwari, A .2009. Isolation, Identification and Quantification of yohimbine alkaloid from the leaves of Rauvolfia

tetraphylla-

an

aphrodisiac

plant.

Medical

Science

Monitor

(Communicated) 23. Kumar, A,; Gaurav, S.S and Acharya, B.K 2008. Determination of total Phytochamicals in Withania somnifera Commercial Extract and Raw herb by High Performance Thin Layer Chromatography, Elsevier Editorial for Phytochemistry (Submitted). 24. Kumar, A, Gaurav, S.S ,:Acharya B.K and Ohdar B, 2008. GC/MS/MS analysis of volatile compounds in the root extract of Withania somnifera, Phytochemistry (Submitted). 25. Kumar, A; 2005, Determination of Toxins and Alkaloids Markers of Toxic Plant from plant Ricinuss communis Linn by new complimentary technique, Published in the proceedings of 57th- Annual meeting of AAFS at New Orleans, USA 26. Kumar, A, Acharya B.K, Gaurav, S.S, Kumar, K, Upadhyay, A. and Ohdar. B. 2009. GC/MS/MS analysis of volatile phytochamicals in plant “Saussurea gossypiphora” called as Sanjeevni, Phytochemistry (Submitted) 27. Kumar, A, Acharya B.K, Gaurav, S.S, Kumar, K, Upadhyay, and Ohdar. B. 2009. Isolation, Characterization and study of carbohydrate binding properties of Lectins from the seeds of Ricinus communis Linn., Progressive agriculture (Submitted) 28. Sharma, B.D. 2005. Myths and Scientific contribution to explore the Astavarga plants. Ayursh Tomorrow, 1 (4): 65-71. 29. Sharma, B.D. 2006. A Complete non-drug cure of hypertension. Current Medical Journal Of India. 12 (3): 65-66. 30. Sharma, B.D. 2006. Management of Cardiovascular diseases through Yoga. Current Medical Journal of India. 11 (12): 62. 31. Sharma, B.D. 2006. The Origin and History of Wheat, barley, gram and potato in Indian Agriculture. In "V.C. Srivastava (ed.) History of Agriculture in India Vol. V. (In Press) Centre for Studies in Civilizations.

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32. Sharma, B.D. and Balkrishan. 2005. Yoga and Joint pains. Current Medical Journal of India 11(1): 54. 33. Sharma, B.D. and Krishan Pal, 2005. Screening for antibacterial and antifungal activity of some medicinal plants of Himachal Himalaya, Univ. J. Phyto & Ayurvedic Heights, 1 (5): 7-10. 34. A.,Kumar, S.S.Gaurav, A.K.Upadhyay, A., Dubey, B., Ohdar, N.M. Sahu, A., Singh, N., Rana. GC/MS studies of the volatile oil of the Indian Ziziphus jujube seeds for new perfume development. Asian J Exp. Bio. Sciences (2009) 35. A.,Kumar, S.S.Gaurav, A.K.Upadhyay, A., Dubey, B., Ohdar, N.M. Sahu, A., Singh, N., Rana. Resveratrol from leaf stalks of Ricinus communis Linn. Asian J Exp. Bio. Sciences (2009) Conference Presentations: National: 1. Balkrishna, Acharya; Upadhyay.A.K., Yadav A.; Physics and physiology of pranayama, in National conference on “Health for all through Ayurveda’ 8-9th Dec.2005, Organized by Deptt. Of Ayurvedic & Unani Services, Govt. of Uttarakhand. 2. Balkrishna, Acharya; Rajiv K., Standardization and Quality control of Ayurvedic medicines, , in National conference on “Health for all through Ayurveda’ 8-9th Dec.2005, Organized by Deptt. Of Ayurvedic & Unani Services, Govt. of Uttarakhand. 3. Balkrishna, Acharya; Prabha.C., Microbiological assessment of medicinal plants, in National conference on “Health for all through Ayurveda’ 8-9 th Dec.2005, Organized by Deptt. Of Ayurvedic & Unani Services, Govt. of Uttarakhand. 4. Kumar, A; 2004,Illicit Use of Different Cough Syrups commonly available in the market as drug of abuse, Published in the proceedings of XV All India Forensic Science Conference, Karnal, India. 5. Kumar, A; 2004,Detection of toxin alkaloids of Ricinuss Communis Linn by newly developed Thin Layer Chromatography, Published in the proceedings of XVI All India Forensic Science Conference,Hyderabad,India.

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6. Kumar, A; 2005, In-Vivo study of ricin toxicity, which produces persistence decrease in SGOT and food intake in rabbit model, Abstract Published in the proceedings of XVII All India Forensic Science Conference,Raipur,India. 7. Kumar, A; 2007,A complete scheme for the analysis of drugs and vegetable poisons

by

molecular

identification

methods

and

newly

developed

chromatographic methods, Published in the proceedings of XVIII All India Forensic Science Conference, Kolkata, India. 8. Acharya Balkrishna, Upadhyay A.K., Yadav A, Physics & Physiology of Pranayama” in “Health for all through Ayurveda” seminar organized by Deptt. Of ISM & H, Govt. of Uttarakhand. 9. Kumar, A, Upadhyay, A.K., New Biotechnologies in Herbal Drug Research, Annual conference of Indian Botanical Society, Allahabad University, 2008. 10. Upadhyay, A.K., Kaushal K. Study and evaluation of some lesser known herbs potentially used as folk medicine by Jaunsari tribe, Annual conference of Indian Botanical Society, Allahabad University, 2008. 11. Upadhyay, A.K., Kaushal K., Exploration of some plant based secret and wisdom of Himalaya, Annual conference of Indian Botanical Society, Allahabad University, 2008. International: 1. R Gokal, A Upadhaya, B Acharyaji, Ramdev Swami. The role of Yog, Pranayam and Ayurved in the prevention and treatment of Chronic Kidney Disease (CKD). 6th Annual Conference on Prevention in Renal disease, Toronto, September 2829, 2007 2. Kumar, A; 2005, Determination of Toxins and Alkaloids Markers of Toxic Plant from plant Ricinuss Communis Linn by new complimentary technique, Published in the proceedings of 57th- Annual meeting of AAFS at New Orleans, USA. 3. Kumar, A; 2005, In-Vivo immunization study of Alkaloids and proteins of toxin plant Ricinuss Communis Linn,17th International meeting of Forensic Scientist in Aug 2005, Hong Kong.

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PATENT FILED (2007-2009) File No. 2114/DEL/2007

Title of Invention An extraction process for Emblica officinalis Gaertn.

Inventers Kumar, A, and Acharya B.K

CBR NO- 5457

A novel method of detection and differentiation of giloy satwa (Tinospora cordifolia) from common adulterants such as potato and maize satwa. A novel herbal liquid solution “Drishti” for various ophthalmic disorders. Detection, Identification and Differentiation of urine of cow of Indian breed amongst the urines of other breed cow urine, goat and buffalo urine. A novel field test kit which enables spot test for the detection and identification of Raw Herbs Active Constituent in the field within a few minutes. “Isolation, Identification and characterization of specific chemical compound marker of 1000.8 KD in shilajit sample responsible for the therapeutic activity of shilajit prescribed in ayurved as panacea”. A novel breathes and posture mechanism which expel the various reactive oxygen species through nasal track. The novel aroma

Kumar, A, and Acharya B.K

94/DEL/2008 CBR NO 279 109/DEL/2008 111/DEL/2008

112/DEL/2008 /DEL/2008

53/DEL/2009 396/DEL/08

composition of more then 50 compounds in specific ratio isolated from Sanjeevni plant ‘Saussurea gossypiphora’ for the prevention and treatment of psychological disorders. Novel floor for the collection and detoxification of cow urine for Ayurvedic therapeutic purposes. Apparatus and method for the housing of toothbrush for prevention and destruction of microbial load deposited onto the bristle and surrounding area of used toothbrushes by novel kit for oral hygiene.

Department of Ayurved Research and Development

Kumar, A, and Acharya B.K Kumar, A, and Acharya B.K Kumar, A, and Acharya B.K Kumar, A, and Acharya B.K

Kumar, A, and Acharya B.K Kumar, A, and Acharya B.K

Kumar, A, and Acharya B.K Kumar, A, and Acharya B.K

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FURTHER RESEARCH PROJECTS 1. Clinical Trials-Phase 1, 11, Ill & IV Stability tests will be conducted on all our products as per ICH (International Conference for Harmonization) guidelines to ensure quality of product through out its shelf life. 2. To conduct study and research projects associated with Yajna, Organic Agriculture, Cow-Urine, Nature and Environment in addition to the study and research in Ayurved. Priority Research project on: 1. Asthma 2. Hypertension 3. Lung Cancer 4. CML 5. Breast Cancer 6. AIDS 7. Diabetes 8. Herbal cosmetic development TECHNICAL COLLABORATION (S) 1. National Institute of Malaria Research (ICMR), Delhi 2. C.C.S. University, Meerut, Uttar Pradesh 3. Central Council for Research in Yoga & Naturopathy, New Delhi (CCRYN) 4. R & D, Divya Pharmacy, Haridwar 5. R & D, Patanjali Ayurved Limited, Haridwar 6. Patanjali & Divya Seva Kendra for Ayurvedic treatment & Research 7. Department of Ayurveda & Unani Services, Haridwar 8. Social Science Research Foundation, Nepal 9. Dev Sanskriti Vishwavidyalaya, Haridwar 10. Indian Society of Medical & Pediatric Oncology Department of Ayurved Research and Development

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11. R & D, Patanjali Foods & Herbal Parks Limited

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