Cv Dr Anand M Jha

Curriculum Vitae Dr. Anand Mohan Jha OFFICIAL ADDRESS Reader & Co-ordinator Department of Biotechnology Samastipur College (Post- Graduate), Samastipur- 848 134, India (Under L. N. Mithila University,Kameshwarnagar,Darbhanga, India)

RESIDENTIAL DDRESS Professor Colony, Bahadurpur, Samastipur- 848101, India Phone No. +916274223078 (R) Mobile No. +919431425273 Mobile No. +919939699549 Email:[email protected]

Date of Birth: 22.nd January 1957

CAREER OBJECTIVES  Teaching and Research  To acquire knowledge about recent developments taking place in the field of biotechnology and disseminate these among the students  To make efforts for bringing the name of institution at International arena. by doing research in the field of Genetic Toxicology and Environmental Mutagenesis

2

EDUCATIONAL QUALIFICATION Ph. D.

Mutagenesis

Title of Thesis

Induction and Isolation of Mutants in French bean (Phaseolus vulgaris L.)

University

Bihar University, Muzaffarpur

Year of Award

1986

Post-graduate

Master of Science in BOTANY

Specialisation

Cytogenetics and Plant Breeding

University

L. N. Mithila University, Kameshwar Nagar, Darbhanga - 846004

Year

1978

Class

First

Percentage of Marks

66.66%

Graduation

Botany Hons.

University

L. N. Mithila University, Kameshwar Nagar, Darbhanga - 846004

Year

1976

Class

Second

Percentage of Marks

57.33%

Under- Graduation

Intermediate Science

University

L. N. Mithila University, Kameshwar Nagar, Darbhanga - 846004

Year

1974

Division

First

Percentage of Marks

60.33%

Secondary Board

Bihar School Examination Board, Patna

Year

1972

Division

First

Percentage of Marks

66.33%

TEACHING EXPERIENCE

3

Under-Graduate

More than 25 Years

Post Graduate

More than 23 Years

 Experience of teaching student of Botany Stream up to Post-graduate level from 1983  Experience of teaching students of Biotechnology stream (B.Sc. Honors Level) from 1999 Botany stream: Biotechnology stream

Plant physiology, Cytogenetics, Molecular biology,Biotechnology Molecular Biology. Genetics, Plant Biotechnology, Recombinant DNA Technology

DISTINCTIONS ACHIEVED 2. Fellow Indian Society of Genetics and Plant Breeding 3. Life Member Environmental Mutagen Society of India 4. Member of Editorial Board of the following International Journals

1. International Journal of Botany- Technical Editor 2. Asian Journal of Scientific Research- Technical Editor 3. Research Journal of Mutagenesis- Technical Editor 4. Research Journal of Environmental Toxicology- Associate Editor 5. Research Journal of Environmental Science- Associate Editor 5. Reviewer of Research Papers submitted for Publication in many International journals such as Mutation Rssearch (Genetic Toxicology and Environmental Mutagenesis), Elsevier Publication International Journal of Botany, International Journal of Biotechnology, Pakistan Journal of Biological Science, ANSI Journals, [email protected]

ADMINISTRATIVE EXPERIENCE Coordinator, Vocational Course in Biotechnology from May 1999- April 2003 and again from April 2006

PARTICIPATION IN TRAINING & SHORT TERM COURSES  Participated in Short ter course and Training in Microbiol Biotechnology and Application at Centre of Relevance and Excellence in Agro and Industrial Biotechnology (TIFAC-CORE)

4 Thapar Institute of Engineering and Technology, Patiala  Paticipated in U.G.C. sponsored INFLIBNET Awareness Programme for the Colleges of Bihar and Jharkhand at St. Xavier College, Ranchi  Participated in Short term training on Genotoxicity Biomarkers in Fishes, at National Bureau of Fish Genetic Resource (NBFGR), Lucknow

RESEARCH ACTIVITIES Area of Specialization:

Genetic Toxicology & Chemical Mutagenesis

Research scholars who have successfully completed their research leading to the Award of Ph.D. degree 1

Dr. S. F. Rab

1993

Cytogenetic Activity of Some Agrochemicals on Vicia faba L.

2

Dr. A. K. Sinha

1996

Mutation Induction for Improved Yield and Oil Content in Indian Mustard (Brassica juncea Czern and Coss)

3

Dr. A. C. Singh

1997

Studies on Mutagenic / Carcinogenic Properties of Some Metallic Compounds

4

Dr. M. Bharti

2000

Assessment of Genotoxic Potential of Some Industrial chemicals in vivo in mice

5

Dr. A. Haque

2002

Recurrent Mutagenesis in Indian Mustard ( Brassica juncea Czern & Coss.

6

Dr. M. Kumar

2008

Mutagenicity of alpha and beta unsaturated aldehydesin mouse in vivo

Research scholars who are registered for Ph.D. degree 1

Ragni

2005

Investigation on Cytogenetical and morphological effects of Arsenic in the irrigation water in agricultural crops,

2

L. K. Ghosh

2005

Exploring the genotoxicity of soil contaminants by employing plant chromosomal aberration assay.

3

S. N. Jha

2006

Assessment of Genotoxicity of some heavy metals

4

J. Choudhary

2007

Assessment of Genetic Effects Of Some Heavy Metals In

5 Freshwater Teleost (Channa punctatus)

Research Projects Undertaken/ Ongoing 1 Biochemical Changes induced by Effluents in Plants. Supported and Financed by U.G.C., New Delhi Grant No. F-8-1 (48)/87 (SR II) (Completed) 2 Mammalian Germ Cell Genotoxicity Of , Unsaturated Aldehydes 2-trans-butenal, 2trans hexanal and 2-trans, 6-cis-nonadienal and Human Risk Assessment. Supported and Financed by U.G.C., New Delhi Grant No. F. No. 3- 121/ 2001 (SR-II), Completed 3 Multi-end point Analysis of Genetic Damage Induced by Arsenic in Mammalian Germ Cells. Supported and Financed by U.G.C., New Delhi Grant No. F. No. 32- 566/ 2006 (SR), (ongoing)

Publications Text Books 1. Comprehensive Aadhunik Vanaspati Shastra for Intermediate Students ; Laxmi Publication (Pvt.) Ltd., New Delhi 2. Comprehensive Paryavaran Adhayan (Hindi) for Class IX of BSEB, Patna ; Laxmi Publication (Pvt.) Ltd., New Delhi 3. Comprehensive Paryavaran Adhayan (Hindi) for Class X of BSEB, Patna; Laxmi Publication (Pvt.) Ltd., New Delhi 4. Comprehensive Biology (Hindi) for Class XI Laxmi Publications (Pvt.) Ltd., New Delhi (in Press).

PUBLICATIONS 1. Anand M. Jha, Akhilesh C. Singh, Uma Sinha and Mithilesh Kumar 2007 Genotoxicity of Crotonaldehyde in the Bone marrow and Germ cells of Laboratory mice, Mutation Rssearch (Genetic Toxicology and Environmental Mutagenesis) , Elsevier, Ireland, 632(1-2), 69-77. .

6 2. Anand M. Jha and Mithilesh Kumar 2006 In vivo evaluation of induction of abnormal sperm morphology in mice by an unsaturated aldehyde crotonaldehyde, Mutation Rssearch (Genetic Toxicology and Environmental Mutagenesis) , Elsevier, Ireland , 603 (2), 159-163. 3. Akhilesh C. Singh, Mithilesh Kumar and Anand M. Jha 2003 Genotoxicity of lomefloxacin an antibacterial drug in somatic and germ cells of Swiss albino mice in vivo. Mutation Rssearch (Genetic Toxicology and Environmental Mutagenesis, Elsevier Publiction, Ireland) , 535, 35- 42 4. A. M. Jha, Akhilesh C. Singh and Mithilesh K. Bharti 2002 Clastogenicity of carbazole in mouse bone marrow cells in vivo. Mutation Research (Genetic Toxicology and Environmental Mutagenesis, Elsevier Publiction, Ireland) , 521, 11- 17. 5. Jha A. M. and M. K. Bharti 2002 Mutagenic profiles of carbazole in the male germ cells of Swiss albino mice. Mutation Research (Genetic Toxicology and Environmental Mutagenesis), Elsevier Publiction, Ireland) , 500, 97- 101 6. Jha, A.M. and Singh, A. C and M. Bharti 1998 Germ cell Mutagenicity of Phthalic acid esters in mice . Mutation Research (Fundamental and Molecular Mechanism of Mutagenesis, Elsevier Publiction, Ireland) 422, 207 - 212. 7. Singh, A.C. Rashmi B. Pandey, Bharti, M. and Jha, A.M. 1997 Cytological effects of two rare earth compounds on the root tip cells of Vicia faba L. J. Environ. Biol. 18 ,27 - 31. 8. Jha, A. M., Rab, S.F. and Singh, A.C. 1996 Cytotoxic activities of three herbicides in root tip cells of Vicia faba L.. J. Environ Biol. 17, 211- 216. 9. Jha, A.M. and Singh, A.C.1995 Clastogenicity of Lanthenides :Induction of chromosomal aberrations in bone marrow cells of mice in vivo. Mutation Research ( Genetic Toxicol. Testing ) 341, 193 - 197. 10. Jha, A. M., Singh, A. C., Jha, N. K. and Rashmi 1994 Cytological effects of water extract of Moringa olefera on Vicia root tip cells. J. Cytol. Genet. 29, 97 -100. 11 Jha, A. M. and Singh, A. C.1994 Clastogenicity of lanthenides:Induction of micronuclei in root tips of Vicia faba. Mutation Research (GenetIc Toxicology Testing, Elsevier Publiction, Ireland) 322, 169-172 12. Jha, A. M. and Sinha, A. K. 1994 Induction of chlorophyll mutations in Brassica juncea Czern & Coss. Acta Botanica Indica 22, 57 - 62. 13 Jha, A. M. and Rab, S. F. 1994 Mitodepressive and chromotoxic effects of herbicides in Vicia faba Acta Botanica Indica 22, 159 - 160. 14. Singh, A.C., Rashmi and Jha, A.M. 1994 Mitodepressive and clastogenic effects of rare earth compouds in plant system . J. Cytol. Genet. 29, 205 - 207 15. Prasad, A. B. and Jha, A. M. 1993 Induced variability for seed yield and seed protein in french bean ( Phaseolus vulgaris L.). J. Cytol. Genet. 28, 31 - 34. 16. Prasad, A. B. and Jha, A. M. 1993 Induced variability for seed protein in french bean ( Phaseolus vulgaris L.). Bioved 4, 121 - 124.

7 17. Jha, A. M. and Sinha, A. K. 1992 EMS and sodium azide induced biological damage in Brassica juncea var. krishna. Acta Botanica Indica 20, 152- 155. 18. Prasad, A. B.and Jha, A. M. 1992 Mutation affecting seed coat colour and seed protein in french bean ( Phaseolus vulgaris L.) . J. Cytol. & Genet. 27, 147- 152. 19. Prasad, A. B. and Jha, A. M. 1989 Laminar genetic disorder induced by gamma rays in different varieties of Phaseolus vulgaris L. J. Ind. Bot. Soc. 68, 241 - 243. 20. Jha, A. M. 1989 Mutagenic effects of cephalexin and sodium azide alone and in combination in Barley (Hordeum vulgare). J. Cytol. Genet. 24, 124- 127. 21. Prasad, A. B., Jha, A.M. and Rashmi 1988 A comparative assessment of radiosensitivity in french bean. J. Cytol. Genet. 25, 140 - 146. 22. Jha, A.M. 1988 Studies on the induced seedling mutants in Phaseolus vulgaris L Acta Botanica Indica 16, 28- 30. 23. Prasad, A.B., Verma, M.P. and Jha, A.M. 1986 Seed protein content and protein pattern in gamma ray induced Phaseolus mutants In: Mutagenesis: Basic and Applied (Proc. Symp. XV Intern. Cong. Genet. Sat. Symp. ) Print House, Lucknow pp. 159- 184. 24. Rab, S.F., Jha, A.M. and Shrivastava, A.K. 1986 mango.Indian Phytopath., 32, 176.

Ceratocystis

fimbriata

on

25. Prasad, A .B., Verma, M. P. and Jha, A. M. 1986 Gamma ray induced Chlorophyll mutation in five varieties of Phaseolus vulgaris L. Acta Botanica Indica 14(spl) 65 -72 26. Prasad, A. B, Verma, M.P. and Jha, A.M. 1984 Seed coat colour as a marker for higher Protein content in Phaseolus vukgaris L. var. Giant Stringless. In: Recent Trends in Botanical Research ( ed. R.N.Gohil), Scientific Publishers, Jodhpur, pp. 278 - 287. Abstracts/ Paper Presented in Seminars/Symposia etc. 27. Prasad, A B., Verma, M. P., Prasad. P. R. and Jha, A.M. 1980 Varietal response to gamma rays in five varieties of French bean. All India Botanical Congress, Abst pp. 116 28. Prasad, A B., Verma, M.P. and Jha, A.M. 1981 Comparative studies of chlorophyll mutations in five varieties of French bean. All India Congress Cytologists and Geneticists, Abst pp. 136 29. Prasad, A B, Verma, M.P. and Jha, A.M. 1982 Laminar Genetic disorder induced by different group of mutagens. Indo- US Seminar on the Effects of Environmental Agents on Genetical System, University of Calcutta, Calcutta, Abst. pp.3. 30. Prasad, A B, Verma, M.P. and Jha, A.M. 1982 Seed coat colour as a marker for higher protein content in Phaseolus vulgaris L. var. Giant stringless. New Approaches in Plant Science Research, Abst pp. 21 31. Prasad, A B, Verma, M.P. and Jha, A.M. 1983 Status of protein in five different varieties of Phaseolus vulgaris L. 1983 Indian Science Congress, Abs. pp. 106 32. Prasad, A B, Verma, M.P. and Jha, A.M. 1983 Modification by mutation of Protein quality and quantity. Abst. XV International Congress of Genetics, New Delhi , 1983 pp. 483

8 33. Prasad, A B, Verma, M.P. and Jha, A.M. 1983 Seed protein content and protein pattern in gamma ray induced Phaseolus mutants. International Congress of Genetics: Satellite Symp on Mutgenesis : Basic and Applied, Abst. pp. 23 - 23 34.Jha, A.M. and Rab, S.F. 1990 Cytological effects of herbicides on root meristem of Vicia faba 1990 L. A. I. C. Cytologists and Geneticists, pp. 23-24 35. Jha, A.M. and Sinha, A. K. 1990 Induced variability for quantitative traits in Indian mustard. 1990 A. I. C. Cytologists and Geneticists, Nagpur University, Nagpur, pp. 24. 36. Jha, A.M. Singh, A. C. and Pandey, R 1994 Genotoxic effects of strontium chloride on root meristem of Allium cepa L. 1994 National Symposium Genes and Human Environment, 1994. 37. Jha, A.M. and Singh, A. C. 1994 Clastogenic effects of lanthanides- induction of micronuclei in root tips of Vicia faba L. 1994 National Symposium Genes and Human Environment, 1994. 38. Jha, A.M. and Singh, A. C. 1994 Clastogenic effects of rare earth compounds in mouse in vivo. 1994 National Symposium Genes and Human Environment, Hyderabad, 1994. 39. Jha, A.M. Singh, A. C. and Jha, N. K. 1994 Terminalia fruit extract induced clastogenic adaptation in root meristem of Vicia faba L. 1994. National Symposium Genes and Human Environment, 1994. 40. Jha, A.M. Singh, A. C. Prasad, A.B. 2002 Genotoxicity of lomefloxacin- an antibacterial drug in somatic and germ cells of Swiss albino mice in vivo. The National Academy of Sciences, India, Abstract,19- 20 41. Jha, A.M. Singh, A. C. and Mithilesh Kumar 2003 Germ Cell Mutagenicity of crotonaldehyde - an unsaturated aldehyde in mice. Environmental Mutagen Society of India Abstract, 76-77. 42.. Bibiliography on Environmental Mutagens ( Work done by Indian workers) Published & Released by Department of Botany, L. N. Mithila University, Darbhanga during XV International Congress of Genetics post Satellite Symposium on Mutagenesis : Basic And Applied, Dec 22- 23, 1983 43. Improvement of Protein Quantity and Quality in Beans, Technical Report U. G. C. Major Research Project ( F 23- 1118/ 79 ( SR II) pp. 101. 44. Monoclonal Antibodies & their applications. Published in Hindustan Times in Competitive Edge, May 2000. 45. Application of Genetic Engineering: Published in special issue of Bharti- a magazine of Samastipur College, Samastipur.2005, pp. 8-10 46. Pramukh Paryavarniya Sankat (in Hindi): Published in special issue of “Bharti” - a magazine of Samastipur College, Samastipur.2005, pp. 1-8 (Hindi section).

Summary of Important Research Findings Published in International and National Journals of Repute

9 1. Clastogenic and mutagenic properties of three herbicides viz. Butachlor, Fluchloralin and Benthiocarb were evaluated in the root meristem, flower buds and morphological and many agronomic traits of Vicia faba L. 2. Chemical mutagens like EMS and Sodium azide were employed to induce mutations for agronomic traits and oil content in in Indian Mustard (Brassica juncea).. 3. Mutagenic and carcinogenic properties of metallic compounds (lanthanides) were studied in plants and animal models and clastogenic properties of lanthanide compounds in plant and animals were reported for the first from our laboratory. The report has been published in an International Journal Mutation Research ( Genetic Toxicol. Testing, , Elsevier Publiction, Ireland ). 4. Genotoxic and mutagenic potentials of industrial chemicals viz. Pthalic acid and carbazole were evaluated in vivo in mice. We have reported that pthalic acid and carbazole are genotoxic and mutagenic for animals in vivo. The results were published in an International Journal Mutation Research (Genetic Toxicol. Testing) and Mutation Research (Fundamental and Molecular Mechanism of Mutagenesis, Elsevier Publiction, Ireland) , 500, 97- 101 5. Recurrent Mutagenesis was successfully employed for inducing mutants with quantitative and qualitative changes in oil content in Indian Mustard ( Brassica juncea Czern & Coss) by the use of gamma rays and EMS.. 6. Evaluation of somatic and germ cell clastogenicity and mutagenicity of alpha and beta unsaturated aldehydes (2-Alkanals) has been undertaken as a major research project keeping in view ubiquitous nature of these organic compounds. The three test chemicals viz. 2-trans butenal, 2-trans hexenal and 2-trans, 6-cis nonadienal induced chromosomal aberrations in the bone marrow cells and also in the germ cells and stage specific dominant lethal mutations as well as high frequency of abnormal sperm heads. Although they have failed to induce higher frequency of heritable chromosomal changes i.e. translocations. The reproductive profile observed in the dominant lethal experiment demonstrates similarity in the effect of these three structurally and functionally related compounds in male germ cells. The primary reproductive effect recorded for these chemicals was induction of dominant lethal mutation resulting in the significant reduction in the number of offspring. The ratio of dominant lethal mutation to heritable translocation has been shown to be dependent upon the nature of chemical mutagens and the stage of post-meiotic germ cells affected. In the present investigation with a and bunsaturated aldehydes higher incidence of dominant lethals were induced in early to late spermatids and testicular sperm but the corresponding frequencies of heritable translocations were much lower. Finally, the human genetic hazards posed by the three alpha and beta unsaturated aldehydes are almost identical. On the basis of the results of the present investigation it may be concluded that the alpha and beta unsaturated aldehydes viz. 2-trans butenal, 2-trans hexenal and 2-trans, 6-cis nonadienal are mutagenic in the germ cells of mammals. The research finding has been published in Mutation Rssearch (Genetic Toxicology and Environmental Mutagenesis) , Elsevier, Ireland , 603 (2), 159-163(2006) and 632(1-2) 2007, 69-77.

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Other Experience 1. Experience of handling sophisticated instruments like PCR, HPLC, GLC, AAS, Fluorescence Microscope, PAGE, UV- Vis Spectrophotometer etc. 2. Practical experience of PAGE Protein analyses, RFLP, Comet assay, Chromosome aberration assay in Plant and Animal models etc

Dr. Anand Mohan Jha