Asianalysis 2009 Kuala Lumpur Poster

Synthesis and Characterization of Peptidyl Fluorescent Probe Dansyl - Glycine -Serine - Glycine - Serine - Methyl Ester ( Dns - Gly - Ser - Gly - Ser - OMe ),Application For Metal Ions Detection Mohd Rashidi Abdull Manap, Nor Azah Yusof *, Siti Mariam Mohd Nor, Faujan Hj Ahmad Department of Chemistry, Faculty of Science Universiti Putra Malaysia,43400 Serdang,Malaysia Corresponding email*: [email protected]

Certain heavy metals can pose potential health risks when consumed by humans due to their high toxicity. Heavy metals such as mercury, lead and cadmium can be released into the environment through industrial use and erosion of natural deposits and once released can contaminate water, soil and air(Jarup.,2003).It is important that we have a sensor that can detect those metals. Some of the peptides that have been applied in metal detection systems were Cys-Pro-Gly-His-Glu (Joshi and Lee., 2008) and GGDGGDGGDGGDGG (White and Holcombe., 2007) where G is Glycine and D is Aspartic Acid. New peptide for sensor development that will be synthesized is a combination of Glycine and Serine and combined with fluorophore Dansyl (Dns) at Nterminal of Glycine. The expected Dns-GSGS will be used to detect metals by monitoring the fluorescence properties.

APPLICATION

SYNTHESIS Boc-Amino Acid

+

Amino Acid-OMe

Boc-Dipeptide-OMe 4 M HCl in Dioxane

DCC,TEA

Crude

Selectivity

Dipeptide-OMe Column Chromatography

Dipeptide-OMe

Boc-Dipeptide-OMe

+

Amino Acid-OMe

Limit of detection

pH study

Boc-Tripeptide-OMe

A new biosensor Dns-Gly-Ser-Gly-Ser-OMe will be synthesized by solution phase technique .Then,it will be applied as a biosensor to detect metal (cation/anion) at optimum parameters such as pH and ligand concentration .Selectivity,detection limit and interference towards certain metal also will determin

Jarup,L. (2003) .Hazards of heavy metal contamination. Br Med Bull. 68: pp 167-182. Joshi,B.P.,Lee,K.-H.(2008).Synthesis of highly selective fluoresecent peptide probes for metal ions:tuning selective metal monitoring with secondary structure.Bioorganic & Medicinal Chemistry.16:pp8501-8509. White,B.R.,Holcombe,J.A.(2007).Fluorescent peptide sensor for the selective detection of Cu2+.Talanta.71 :pp 2015–2020.

Thank you to Ministry of Science, Technology & Innovation, Malaysia (MOSTI) for financial support under Science Fund 03-01-04-SF0134 (5450218)