8-rbc Fragility

( Lab. no. 8 )

Osmotic fragility of RBCs

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Types of Solutions: Hypotonic Sol. Isotonic Sol. Hypertonic Sol.

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Principle: The responses of RBCs to solutions of different osmotic activities are examined after one hour of adding fresh blood to these solutions.

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Procedure:  Add (1–2) drops of fresh blood to solutions of NaCl with concen.:(0.1%, 0.3%, 0.45%, 0.7%, 0.9%, and 10%).

 Add (1–2) drops of fresh blood to solutions of Glucose (1%, 5%, and 10%).

 After one hour examine the response of RBCs to the different solutions of NaCl and Glucose.

 Microscopic examinations may be performed.

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NaCl 0.1%

0.3%

0.5%

0.7%

0.9%

Glucose

1%

5%

10%

10%

NaCl 0.7%

0.9%



0.5%



0.3%



0.1%

5%

10%

Glucose

1%

10%

12 3

9 6

After ONE hour [email protected]

0.1-0.33% NaCl 1.0%

Glucose

very hypotonic sol.

 True solution.  No supernatant.  Transparent.  Complete hemolysis.

0.33%-0.48% NaCl hypotonic solution

 Mixture at the beginning is turbid. Supernatant (yellowish or reddish clear and transparent). Partial haemolysis

0.5%-0.7%-0.9%-10% NaCl 5%-10% Glucose Slightly hypotonic Isotonic and Hypertonic solution

 Mixture at the beginning is turbid. Supernatant colorless. No haemolysis

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