The Effect Of Ph On Enzymatic Activity

The effect of pH on enzymatic activity: Table: Optical density over time for pH 3, 5, and 7. Time 3

pH 5

7

0 0.207 -0.100 0.056 15 0.226 -0.100 0.091 30 0.245 -0.890 0.135 45 0.263 -0.060 0.172 60 0.281 -0.035 0.202 75 0.298 -0.014 0.228 90 0.313 0.007 0.255 105 0.328 0.022 0.272 120 0.342 0.036 0.287 135 0.355 0.048 0.302 150 0.367 0.058 0.315 165 0.379 0.068 0.327 180 0.389 0.074 0.337 195 0.400 0.080 0.347 210 0.410 0.085 0.355 225 0.419 0.090 0.364 240 0.427 0.093 0.371 255 0.435 0.095 0.377 270 0.442 0.098 0.383 285 0.449 0.100 0.389 300 0.455 0.102 0.393 Observation: Color of mixture in cuvette after Spectrophotometric measurement pH 3: caramel color pH 5: light orange color pH 7: light orange-brown