Stains Comparison
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Stain 1 Stain 2 Wavelength, nm Intensity Wavelength, nm Intensity 420 5.2 420 2.56 423 5.37 423.84 2.24 426 5.55 427.68 2.06 429 5.75 431.53 2.1 432 5.95 435.37 1.98 435 6.36 439.21 1.98 438 6.96 443.05 1.9 441 7.88 446.89 1.86 444 8.85 450.74 1.93 447 9.68 454.58 2.01 450 10.8 458.42 2.16 453 12.11 462.26 2.24 456 13.69 466.11 2.38 459 15.58 469.95 2.33 462 17.36 473.79 2.42 465 19.72 477.63 2.45 468 22.52 481.47 2.37 471 25.13 485.32 2.57 474 29.27 489.16 2.63 477 32.15 493 2.8 480 35.08 496.84 2.99 483 40.47 500.68 3.12 486 43.73 504.53 3.31 489 47.23 508.37 3.75 492 48.56 512.21 4.02 495 54.87 516.05 4.3 498 57.79 519.89 4.88 501 61.53 523.74 5.47 504 61.36 527.58 5.8 507 66.99 531.42 6.69 510 69.06 535.26 7.18 513 70.24 539.11 7.75 516 68.35 542.95 8.42 519 72.16 546.79 9.33 522 72 550.63 9.96 525 70.08 554.47 11.04 528 68.95 558.32 11.91 531 69.91 562.16 13.45 534 68.22 566 15.66 537 68.05 569.84 17 540 63.96 573.68 19.05 543 60.65 577.53 20.39 546 59.44 581.37 24.06 549 59.22 585.21 26.04 552 55.7 589.05 27.92 555 52.07 592.89 29.78 558 50.81 596.74 30.22 561 45.18 600.58 32.45 564 46.01 604.42 34.46 567 44.09 608.26 35.11 570 41.33 612.11 34.22 573 38.6 615.95 34.36
576 579 582 585 588 591 594 597 600 603 606 609 612 615 618 621 624 627 630 633 636 639 642 645 648 651 654 657 660 663 666 669 672 675 678 681 684 687 690 693 696 699 702 705 708 711 714 717 720 723 726 729 732 735
36.54 33.14 33.39 31.88 29.63 26.64 25.32 23.29 22 20.71 20.46 19.14 18.08 16.85 15.03 13.89 14.39 13.32 12.45 12.31 11.28 10.39 9.83 8.99 9.11 8.82 8.54 8.12 7.92 7.33 6.87 6.74 6.3 6.26 6.13 6.06 5.9 5.79 5.59 5.55 5.3 5.19 5.16 5.03 4.96 4.87 4.8 4.77 4.73 4.67 4.63 4.57 4.48 4.46
619.79 623.63 627.47 631.32 635.16 639 642.84 646.68 650.53 654.37 658.21 662.05 665.89 669.74 673.58 677.42 681.26 685.11 688.95 692.79 696.63 700.47 704.32 708.16 712 715.84 719.68 723.53 727.37 731.21 735.05 738.89 742.74 746.58 750.42 754.26 758.11 761.95 765.79 769.63 773.47 777.32 781.16 785
32.87 34.94 33.88 33.49 33.15 31.28 28.71 27.73 30.71 28.9 28.34 27.01 23.1 22.31 20.64 20.5 19.59 19.25 18.13 16.06 14.56 13.44 12.45 10.25 11.75 11.13 10.34 9.57 9.01 8.27 7.42 6.76 6.3 5.72 6.31 5.86 5.41 5 4.8 4.69 4.13 3.93 3.25 2.5
738 741 744 747 750 753 756 759 762 765 768 771 774 777 780 783
4.48 4.41 4.39 4.37 4.38 4.37 4.35 4.33 4.29 4.27 4.27 4.25 4.23 4.2 4.17 4.16
Stain 3 Replace data below with your data Wavelength, nm Intensity 500 2.07 75 503 1.97 506 1.93 70 509 1.95 65 512 1.96 515 2.08 60 518 2.29 55 521 2.42 524 2.66 50 527 2.84 45 530 2.83 533 3.01 40 536 3.12 539 3.37 35 542 3.61 30 545 3.73 548 4.02 25 551 4.49 20 554 4.77 557 5.16 15 560 5.31 10 563 6.03 566 6.48 5 569 6.76 0 572 7.03 400 425 450 575 7.56 578 7.68 581 8.63 584 9.27 587 9.71 590 10.29 593 10.4 596 10.9 599 10.97 602 12.56 605 12.56 608 12.78 611 13 614 12.63 617 12.86 620 12.31 623 12.45 626 12.66 629 12.96 632 12.99 635 12.54 638 11.73 641 11.43 644 11.09 647 10.49 650 11.43 653 11.31
475
500
525
550
575
6
656 659 662 665 668 671 674 677 680 683 686 689 692 695 698 701 704 707 710 713 716 719 722 725 728 731 734 737 740 743 746 749 752 755 758 761 764 767 770 773 776 779 782 785
11.25 11.31 10.28 9.02 8.89 8.3 7.93 7.82 8.03 7.5 7.26 7.1 6.13 5.98 5.41 5.39 4.93 4.73 4.57 4.8 4.53 4.36 4.09 4.03 3.65 3.46 3.1 3.13 2.9 2.7 2.56 2.59 2.67 2.44 2.43 2.3 2.21 2.2 2.09 1.88 1.76 1.75 1.53 1.36
37.5 35 32.5 30 27.5 25 22.5 20
Column B
17.5
Column D
15 12.5 10 7.5 5 2.5 500
525
550
575
600
625
650
675
700
725
750
775
0 800
Column F
37.5 35 32.5 30 27.5 25 22.5 20
Column B
17.5
Column D
15 12.5 10 7.5 5 2.5
0 0
Column F
576 579 582 585 588 591 594 597 600 603 606 609 612 615 618 621 624 627 630 633 636 639 642 645 648 651 654 657 660 663 666 669 672 675 678 681 684 687 690 693 696 699 702 705 708 711 714 717 720 723 726 729 732 735
36.54 33.14 33.39 31.88 29.63 26.64 25.32 23.29 22 20.71 20.46 19.14 18.08 16.85 15.03 13.89 14.39 13.32 12.45 12.31 11.28 10.39 9.83 8.99 9.11 8.82 8.54 8.12 7.92 7.33 6.87 6.74 6.3 6.26 6.13 6.06 5.9 5.79 5.59 5.55 5.3 5.19 5.16 5.03 4.96 4.87 4.8 4.77 4.73 4.67 4.63 4.57 4.48 4.46
619.79 623.63 627.47 631.32 635.16 639 642.84 646.68 650.53 654.37 658.21 662.05 665.89 669.74 673.58 677.42 681.26 685.11 688.95 692.79 696.63 700.47 704.32 708.16 712 715.84 719.68 723.53 727.37 731.21 735.05 738.89 742.74 746.58 750.42 754.26 758.11 761.95 765.79 769.63 773.47 777.32 781.16 785
32.87 34.94 33.88 33.49 33.15 31.28 28.71 27.73 30.71 28.9 28.34 27.01 23.1 22.31 20.64 20.5 19.59 19.25 18.13 16.06 14.56 13.44 12.45 10.25 11.75 11.13 10.34 9.57 9.01 8.27 7.42 6.76 6.3 5.72 6.31 5.86 5.41 5 4.8 4.69 4.13 3.93 3.25 2.5
738 741 744 747 750 753 756 759 762 765 768 771 774 777 780 783
4.48 4.41 4.39 4.37 4.38 4.37 4.35 4.33 4.29 4.27 4.27 4.25 4.23 4.2 4.17 4.16
Stain 3 Replace data below with your data Wavelength, nm Intensity 500 2.07 75 503 1.97 506 1.93 70 509 1.95 65 512 1.96 515 2.08 60 518 2.29 55 521 2.42 524 2.66 50 527 2.84 45 530 2.83 533 3.01 40 536 3.12 539 3.37 35 542 3.61 30 545 3.73 548 4.02 25 551 4.49 20 554 4.77 557 5.16 15 560 5.31 10 563 6.03 566 6.48 5 569 6.76 0 572 7.03 400 425 450 575 7.56 578 7.68 581 8.63 584 9.27 587 9.71 590 10.29 593 10.4 596 10.9 599 10.97 602 12.56 605 12.56 608 12.78 611 13 614 12.63 617 12.86 620 12.31 623 12.45 626 12.66 629 12.96 632 12.99 635 12.54 638 11.73 641 11.43 644 11.09 647 10.49 650 11.43 653 11.31
475
500
525
550
575
6
656 659 662 665 668 671 674 677 680 683 686 689 692 695 698 701 704 707 710 713 716 719 722 725 728 731 734 737 740 743 746 749 752 755 758 761 764 767 770 773 776 779 782 785
11.25 11.31 10.28 9.02 8.89 8.3 7.93 7.82 8.03 7.5 7.26 7.1 6.13 5.98 5.41 5.39 4.93 4.73 4.57 4.8 4.53 4.36 4.09 4.03 3.65 3.46 3.1 3.13 2.9 2.7 2.56 2.59 2.67 2.44 2.43 2.3 2.21 2.2 2.09 1.88 1.76 1.75 1.53 1.36
37.5 35 32.5 30 27.5 25 22.5 20
Column B
17.5
Column D
15 12.5 10 7.5 5 2.5 500
525
550
575
600
625
650
675
700
725
750
775
0 800
Column F
37.5 35 32.5 30 27.5 25 22.5 20
Column B
17.5
Column D
15 12.5 10 7.5 5 2.5
0 0
Column F
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