Salmonell And Shigella Lab-13
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Salmonella and shigella
Salmonella clinical sps. 1-faeces 2-rectal swab *culture (XLD,HE and MacConkey(selective media)) *selenite F broth (enrichment media) 3-blood culture
Lab diagnosis *gram negative bacilli, *motile, *NF, *API20E, *TSI (H2S) *semi-automated and automated systems (vitek system,microscan system,PCR)
1-slide agglutination 2-tube agglutination (WIDAL test) it is a tube agglutination test for the detection of antibodies against entric fever. Serum aggl rise sharply during the second and third week of salmonella infiction . *(serotyping (autbreaks of food poisoning) *(phagetyping and biotyping (to see the orgin type)
Technique 1-make serial dilutions of the patints serum in saline in several raws 2-to each of these add equal volumes of the antigen suspensions(o,H) for typhoid paratyphoid A ,B and C 3-shake will put in water bath at 37C for 4 hours *serial dilution dilute the serum in 1/10, 1/20, 1/40, 1/80, 1/160 ,1/320, 1/640
shigella LABORATORY INDICATIONS: *non-motile *-/+ TSI reaction (no gas) *NF none-lactoes feremont
The End
Salmonella clinical sps. 1-faeces 2-rectal swab *culture (XLD,HE and MacConkey(selective media)) *selenite F broth (enrichment media) 3-blood culture
Lab diagnosis *gram negative bacilli, *motile, *NF, *API20E, *TSI (H2S) *semi-automated and automated systems (vitek system,microscan system,PCR)
1-slide agglutination 2-tube agglutination (WIDAL test) it is a tube agglutination test for the detection of antibodies against entric fever. Serum aggl rise sharply during the second and third week of salmonella infiction . *(serotyping (autbreaks of food poisoning) *(phagetyping and biotyping (to see the orgin type)
Technique 1-make serial dilutions of the patints serum in saline in several raws 2-to each of these add equal volumes of the antigen suspensions(o,H) for typhoid paratyphoid A ,B and C 3-shake will put in water bath at 37C for 4 hours *serial dilution dilute the serum in 1/10, 1/20, 1/40, 1/80, 1/160 ,1/320, 1/640
shigella LABORATORY INDICATIONS: *non-motile *-/+ TSI reaction (no gas) *NF none-lactoes feremont
The End
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