Relatorio Bioquimica.docx
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Massa de proteína (μg) Abs595nm 2
0,043
4
0,061
6
0,070
8
0,075
10
0,114
12
0,116
14
0,156
16
0,180
Curva-Padrão de Albumina 0.2 0.18
y = 0.0096x + 0.0156 R² = 0.9504
0.16
Abs595nm
0.14 0.12 0.1 0.08 0.06 0.04 0.02 0 0
2
4
6
8
10
Massa de proteína (μg)
12
14
16
18
para-nitrofenolato (nmoles)
Abs420nm
0,1
0,023
0,2
0,052
0,4
0,121
0,6
0,197
1,0
0,323
1,5
0,486
2,0
0,626
2,5
0,790
3,0
1,038
3,5
1,190
4,0
1,501
Curva-Padrão de p-nitrofenolato 1.6 y = 0.3593x - 0.037 R² = 0.9916
1.4 1.2
Abs420nm
1 0.8
0.6 0.4 0.2 0 -0.2
0
0.5
1
1.5
2
2.5
p-nitrofenolato (nmoles)
3
3.5
4
4.5
0,043
4
0,061
6
0,070
8
0,075
10
0,114
12
0,116
14
0,156
16
0,180
Curva-Padrão de Albumina 0.2 0.18
y = 0.0096x + 0.0156 R² = 0.9504
0.16
Abs595nm
0.14 0.12 0.1 0.08 0.06 0.04 0.02 0 0
2
4
6
8
10
Massa de proteína (μg)
12
14
16
18
para-nitrofenolato (nmoles)
Abs420nm
0,1
0,023
0,2
0,052
0,4
0,121
0,6
0,197
1,0
0,323
1,5
0,486
2,0
0,626
2,5
0,790
3,0
1,038
3,5
1,190
4,0
1,501
Curva-Padrão de p-nitrofenolato 1.6 y = 0.3593x - 0.037 R² = 0.9916
1.4 1.2
Abs420nm
1 0.8
0.6 0.4 0.2 0 -0.2
0
0.5
1
1.5
2
2.5
p-nitrofenolato (nmoles)
3
3.5
4
4.5
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