Recallsmedtech.docx

Q: What are the cold factors? A: "Malamig sa 7/11 (Factor VII, Factor XI)" Kikiligin ka ba kung sasabihin ko sayong ikaw ay protein sa buhay ko? Kasiiiii... protein is from the Greek word PROTEIOS meaning "of primary importance". Trisomy 18-> edward syndrome Trisomy 21-> down syndrome Trisomy 13-> patau syndrome 13 years old si Patau and 18 years old naman si Edward nang magka Down Syndrome sila noong 21st. Spherocyte ka ba? Ang rupok mo kasi. Clinical Parasitology by Beaver Template for PCR? A. RNA B. ssDNA C. dsDNA D. None

Anong parasite ang kelangan i-massage? edi N. gonorrhoeae bakit? kasi siya yung may Pili.. Oops, thank you, next. Long buccal capsule but short genital primordium or Short buccal capsule but large genital primordium? Ancylostoma duodenale o Strongyloides stercoralis? “Malaki bibig pero juts o maliit bibig pero daks?” Type of RBCs infected by Malaria. P. Vivax = Vata! (young RBCs) P. Ovale = Oo Vata rin! (young RBCs) P. Malariae = Matanda (old RBCs) P. Falciparum = walang Finifili (all RBCs) Oxides + nonmetals with water = ACIDS Oxides + metals with water = BASES Staff: Para saan si Modified Thayer Martin? Me: Used to culture and isolate Neisseria spp. Staff: Ano yung selective supplement? M: *mabilis na sumagot* Vancomycin, Colistin, Nystatin and Trimetoprim lactate Lahat sa lab: WOW! Glycogen Storage Dse. "Vice President Cory Aquino Meet Her Tita" (c) "God Loves Devil Because Man's LIer Fvck" I-Von Gierke = G6PD II-Pompe = Lysosomal III-Cori = Debranching IV-Andersen = Branching V-McArdle = Muscle phosphorylase VI-Hers = Liver VII-Tarui = Fructokinase Immunology Complement System: C4b + C2b = C3-convertase C4b + C2b + C3b = C5-convertase C5b + C6 + C7 +C8 + C9 = MAC development BEST HONING STONE • Belgium Yellow MORE POLISHING EFFECT • Arkansas BADLY KNICKED KNIFE • Fine Carborundum STROPPING • removal of burr • toe to heel • polish and sharpen the cutting edge

(H)ONING • removal of gross nicks • (H)eel to toe • to remove irregularities from the knife BEVEL ANGLE - 27 to 32°C WEDGE ANGLE - 14 to 15°C CLEARANCE ANGLE - 5 to 15°C PLANE CONCAVE KNIFE - one side is flat, the other is concave; 25mm BICONCAVE KNIFE - both sides concave; 120mm PLANE WEDGE KNIFE - both sides straight; 100mm CLEARING • Xylene - most rapid & most commonly used • Toluene - substitute for xylene • Benzene - causes aplastic anemia • Chloroform - tough tissue spx, prolonged inhalation can cause Liver damage • Cedarwood Oil - for CNS specimen • Aniline Oil - fine & delicate spx REPORTING CANCER CYTOLOGY SMEARS (Key word only) CLASS I - Absence CLASS II - No evidence CLASS III - Suggestive CLASS IV - Strongly suggestive CLASS V – Conclusive HAXI VDOY ✓ Horizontal ✓ Abscissa ✓ X axis ✓ Independent > Vertical > Dependent > Ordinate > Y axis Performs dissection during autopsy • prosector 1. BIOPSY • Incisional biopsy - removal of part • Excisional biopsy - entire tissue • Needle biopsy - uses a specialiaze needle • Aspiration biopsy - uses an ordinary needle • Smear/Exfoliative cytology - alter cell layer • Bite biopsy - uses specialize forcep DEGENERATIVE CHANGES A. Metaplasia - transformation from one adult cell type to another B. Dysplasia - change in cellular shape, size and orientation C. Anaplasia - from adult cell type to primitive form D. Neoplasia - tumor formation NUCLEAR CHANGES • PYKNOSIS - condensation of the nucleus • KARYORRHEXIS - fragmentation of the nucleus • KARYOLYSIS - dissolution of the nucleus [SOMATIC DEATH] ~ 2ndary changes I. Pallor mortis - pale skin color II. Algor mortis - cooling ō ē body III. Rigor mortis - stiffining ō ē skeletal muscle IV. Livor mortis - purplish skin discoloration V. Putrefaction - foul odor VI. Post mortem clotting - currant jelly-like VII. Dessication - drying of the skin and eyes VIII. Autolysis - cell decomposition by its self using enzyme IX. Skeletonization - skeleton formation LIVOR MORTIS • oozing of blood on incision • disappears when pressure is applied but reappears when pressure is released ECCHYMOSIS • no oozing of blood on incision • appears when pressure is applied but disappears when pressure is released [CELLULAR DEATH] ✓ APOPTOSIS - programmed cell death ✓ NECROBIOSIS - physiologic cell death ✓ NECROSIS - pathologic cell death

Guidelines for record and specimen retention: [RECORDS] • Bone Marrow reports, Clinical & Surgical pathology laboratory reports - 10 YEARS • Cytogenetics reports - 20 YEARS • Autopsy forensic reports - INDEFINITELY Guidelines for record and specimen retention: [SPECIMENS] • Cytogenetic slides - 3 YEARS • Pathology blocks and Bone marrow slides - 10 YEARS • Cytogenetic diagnostic images - 20 YEARS FROZEN SECTION [COLD KNIFE] ✓ carbon monoxide [CRYOSTAT] ✓ refrigerated cabinet which houses a microtome ✓ Rotary ✓-18 to -20°C Releasing of Results • Surgical pathology and Cytology - 24hrs • Frozen section - 5 to 15mins • Autopsy report - within 1 week MAIN FACTORS INVOLVED IN FIXATION • pH: 6-8 • temperature: routine (RT), 0-4°C (OT) • thickness of section: 4-6 micra; renal biopsy 2-4 micra • duration of fixation: 24hours • volume: 10-20:1 FORMALDEHYDE FIXATIVE ✓ Formaldehyde - most common ✓ 10% Formol Saline - for CNS ✓ 10% Neutral Buffered Formalin - universal fixative ✓Alcoholic Formalin (Gendre's) - coagulates sputum ✓ Formol Calcium - for Lipids METALLIC FIXATIVE [Mercuric Chloride] - most common mettalic fixative ✓ Zenker's Fluid - for trichrome staining ✓ Zenker-Formol (Helly's) - for pituitary glands & bone marrow ✓ Heidenhain's Susa - for tumor biopsies eps. the skin ✓ B-5 fixative - used for bone marrow [Chromate Fixative] PORC ✓ Potassium Dichromate ✓ Orth's Fluid - for eary degenerative process & tissue necrosis ✓ Regaud's Fluid (Muller's) - for mitochondria, chromosomes & golgi bodies ✓ Chromic acid [Lead Fixative] - for acid mucopolysaccharide PICRIC ACID FIXATIVES - excellent for glycogen demonstration, explosive and may impart yellow color to tissue ✓ Bouin's Solution - for embryo & pituitary biopsies ✓ Brasil's Alcoholic Picroformol ALCOHOL Methanol - dry & wet smears, blood smears and bone marrow tissue Isopropyl alcohol touch preparation Ethanol - most common & routinely used Carnoy's Fluid - most rapid fixative; used for chromosomes, lymph glands & urgent biopsies Newcomer's Fluid ACETONE - for enzyme studies and for brain studies e.g. RABIES VACUUM EMBEDDING TECHNIQUE • this is the most rapid embedding technique

Term of PRC Commissioner • 7 YEARS (PD 223)