Presentation.pptx

Protein microarrays

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Levels of Organization • Organism – A single complete individual • Organ System – Group of organs that perform a specific purpose in the human body • Organs – Structure composed of two or more tissues types to perform a specific function • Tissues – Clusters of cells performing a similar function • Cells – The basic and the smallest unit of life. The study of cell organelles is called Cytology • Organelles – Microscopic structures in a cell that carry out its individual functions • Molecules – Make up organelles and other cellular components • Atoms – The smallest particle with unique chemical identities 2

Applications of protein arrays

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Aberrant expression of self proteins leading to autoantibody production Protein A

Immune system: Everything is self! No harm!!

Protein A

Immune system: Cannot recognise protein A!! Kill this new molecule! Antibody to be produced

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HuProt array Full length recombinant human protein with GST tag

GST tag

Protein

Immobilize on chip

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Autoantibody screening for detection of Biomarker Healthy individual

Patient

Serum antibodies

Serum antibodies

HuProt array

HuProt array

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