Pig Men Tech Shrimps

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PIGMENTECH: A NATURAL ALTERNATIVE FOR SHRIMP PIGMENTATION Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

1

Pigmentation trial with a natural mix

PIGMENTECH

Experiment made in AQUACOP, Tahiti Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

2

pigmentech

specification

• Crude Protein 52% • Crude fat 10% • Rich in Pigments • Rich in Essential Fatty Acids

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

3

pigmentech

Experimental design

• Penaeids (L.stylirostris) IBW 22,5g (In.colour=28) • Tank, Clear water – T° 26°C, S= 36%° • 3 Incorporations rate • 6 replicates • 21 days duration • 3-4 feed distribution / d • Colour index assessment (3min boiling -5g/l) • Weight gain

• Feed : (35/9) – Fish meal – Wheat – Soya cake – Yeast – Wheat gluten – Fish oil

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

4

WEIGHT GAIN (g) .

pigmentech Results: Growth 3,5 3 2,5 2 1,5 1 0,5 0 Control

4%

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

8% 5

pigmentech Results: Growth • Significant growth improvement (digestible protein) • No survival differences (80-100% according tanks) • No differences in moult amount

• No difference in food consumption Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

6

pigmentech Results: coloration

Cont 4% 26,4 28,3 +/-1,3a Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

+/-1,1b

8% 30,2 +/-0,9 c 7

pigmentech Results : PIGMENTATION

Control 0 ppm xanthophylls

Epiderm (ppm astaxanthine)

Hepathopancreas (ppm astaxanthine)

510

28

PIGMENTECH 4% 93 ppm xanthophylls

890

PIGMENTECH 8% 189 ppm xanthophylls

1383

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

119

156

8

pigmentech Results : PIGMENTATION FSignificant improved coloration vs Control FIncreased colour with increase incorporation rate FPIGMENTECH’s pigments (lutein, carotene,…) turns into astaxanthine

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

9

pigmentech Pigments conversion alpha carotene

beta carotene

beta cryptoxanthine alpha cryptoxanthine

iso-cryptoxanthine echinenone

luteine

Katayama 1971,1972 Tanaka 1976

zeaxanthine

astaxanthine

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

canthaxanthine

10

pigmentech Pigments conversion alpha carotene

beta carotene

50 %

beta cryptoxanthine alpha cryptoxanthine

iso-cryptoxanthine echinenone

luteine Yamada 1990 Negre Sardagues 1993 Chien 1992

zeaxanthine

astaxanthine

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

canthaxanthine 80 %

11

pigmentech Pigments conversion luteine

zeaxanthine

beta carotene

80 % 50 %

760 ppm

astaxanthine

Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

250 ppm

12

pigmentech Conclusion FShrimp colour can be maintained and improved with PIGMENTECH , bringing pigments with other interesting nutrients: 9Highly digestible protein 9Natural anti oxidants 9Essential fatty acids

FEconomical product Nicolas ROBERT NUTRIWAY AQUA TECHNA – Route de St-Etienne-de-Montluc – Les Landes de Bauche - 44220 COUËRON –/ FRANCE Tél. (33)TECHNA 02 40 85 –41Route 41 – Fax (33) 02 40 86 44 32 – Les Landes de Bauche - 44220 COUËRON – FRANCE AQUA de St-Etienne-de-Montluc Tél (33) 02 40 85 41 41 – Fax (33) 02 40 86 44 32

13

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