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  • November 2019
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Film of rustyred sputum shows typical organisms, many PMN’s and RBC’s -

-Fresh

emulsified sputum mixed with antiserum gives capsule swelling (Quellung reaction) -peritoneal exudates can also be used

-Streptococcus

pneumoniae Quellung (capsular swelling) reaction can be used to demonstrate the presence of a specific capsular type of the bacterium.

-BAP in CO2 or candle jar; blood culture

--Streptococcus pneumoniae A mucoid strain on blood agar showing alpha hemolysis (green zone surrounding colonies). Note the zone of inhibition around a filter paper disc impregnated with optochin. Viridans streptococci are not inhibited by

  Animals die in 18-48 hours; heart blood gives pure culture; sensitive but seldom used because of the need to maintain a mouse colony.

 

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