Nayak Cvpdf

Archana Nayak 33 Battery Park Ave, Greenock, Renfrewshire PA16 UK [email protected], mobile 07771211563 landline 01475717984 Personal Attributes • Versatile, motivated and geared to achieve excellence in research and academics. • Enthusiastic team player and committed to career ACADEMIC QUALIFICATIONS 2005-2007 2002-2005 2000-2002 2000 2007

M.Sc in Biochemistry, Mysore University (A Central University) Mysore securing 80%. B.Sc with Biotechnology as Honors, other subjects included Chemistry and Zoology from Mount Carmel College, Bangalore securing 85%. 10+2 with Physics, Chemistry, Biology and Mathematics from MES Kishore Kendra College, Bangalore securing 88% SSLC securing 91.84% Computer proficiency with language C from University of Mysore.

Employment History June/2007 – September/2009 Company name: Molecular Connections Pvt. Ltd. Molecular Connections Company is a pioneering in silico discovery Services and database development Company based in Bangalore, India. I entered the organization as a junior research associate in the Curex team. During my time at Curex I worked on core cell signaling project focusing on diabetes and neurological disorders. My responsibilities involved: • • • • • •

Studying scientific journals with attention to the details. Interpreting the results with reference to standard databases Building cell signaling cascades and pathway networks. Running a bioinformatics tool to accommodate data. Assuring quality work with high targets and delivered on time to the customer. Valuable skills gained from this work included excellent organizational and communication skills as I was responsible for periodical presentation of the technical data and report writing for the same.

After an appraisal as a senior scientific analyst my duties served the services as well as product development team simultaneously.

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In the product domain, apart from the regular curation we undertook tasks to improvise the product like building chat window, reference database for internal use to speed up team work, and other motivational initiative thus adding a creative spirit to the job assigned. Well versed with search engines like Pub med, Swiss prot, Mesh, HGNC, iHOP and many others we identify cell markers for assay development and drug discovery. This provides a good insight into the pathologies of the diseases and gives way to excellent data interpretation skills.

PROJECT EXPERIENCE M.SC Academic project Jan 2007 – may 2007:

University of Mysore, Mysore

Title: “partial purification and characterization of PROTEASES from Hippasa aglinoides. (Funnel web spiders) under the guidance of Dr.kemparaj. Abstract: In this project effort has been made to characterize the proteolytic activity of the crude venom & partially purify the protease using column chromatography. Initially whole venom has been characterized using SDS PAGE. The dose dependent caseinolytic activity of the venom and Zymograms were done to support the data. The venom showed a procoagulant activity as measured by the prothrombin time. The proteolytic fraction was then run into the CM-Sephardex column to partially purify the enzyme. Summer training research projects: July 2006: Indian institute of science, Bangalore, India Title: H-1 NMR assignments and secondary structure identification of human ubiquitin protein and purification and analysis of protein under the guidance of Dr.Siddaharth Sarma. Abstract: The sample protein was extracted using French pressure, subjected to high speed centrifugation then purified using ammonium sulphate precipitation followed by dialysis and then chromatographic techniques also crosschecked by HPLC. The purity of sample protein and the analysis demonstrated using SDS PAGE. The NMR spectrum analysis was carried out using ubiquitin protein. I have assigned the ubiquitin proton NMR spectrum using the sequential assignment method. Also using the software ANSIG, the spectrum was viewed as 2D plane out of any 3D or 4D spectrum that is displayed .the peak picking and assignment of cross peaks is done after checking the chemical shift values that fall on the vertical line as in TOCSY because we see a total correlation of NH HA HB HG and so on. Also, the secondary structure of a sample protein was analyzed using the TALOS program The Chemical Shift Index

was yet another complimentary technique used for secondary structure analysis. Projects done at the B.Sc level: •

Jan-March 2005 Literature survey and report with seminar presentation on topic like molecular oncology and calcium regulation.

Speed of proliferation is always been a challenging topic of interest as I took oncology in my final year B.Sc report writing. Extensive database collection and presentation of facts honed my skills in the same. April-July 2004 On job training course in Techniques in Immunology, Jain Institute of Vocational and Advanced studies Hands on experience to conduct ELISA, ODD, RID, IEP, RIEP, CCIEP and basic medical immunology techniques- WIDAL, HCG etc •

On job training course in Clinical Biochemistry, 1 month, Shanthi Diagnostic Centre.

Skilled to conduct Gastric function tests and liver function tests. Urine analysis-(qualitative and quantitative) Blood analysis--(qualitative and quantitative) and patients report management and many responsibilities associated with the lab. TECHNIQUES KNOWN •

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Analytical and separation techniques: Paper chromatography, TLC, column chromatography, Gel electrophoresis-SDS and native page (Zymograms), Ultracentrifugation, UV spectrophotometer, Sox let extraction, Distillation. Microbiological techniques: Sterilization of glass wares, media preparation, Isolation culture staining of microorganisms. Enzymological techniques: Extraction, purification and characterization of enzymes. Kinetic assay and end point assay of parameters such as Time, substrate concentration, etc(enzymes like Alkaline Phosphates, Invertase& amylase) Genetics and molecular biology: Identification of drosophila mutants, single mutant crosses, Isolation of DNA and RNA from biological sources. Quantitative estimation of DNA and RNA. Molecular biology and genetic engineering: Mini and large scale plasmid isolation, Western blotting, Lambda phage restriction digestion. Animal studies: Edema ,myotoxcity , tissue necrosis

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Instruments familiar with: Spectrophotometer, HPLC, NMR

Awards, Seminars presented and conferences attended: • • • •

NATIONAL MERIT SCHOLARSHIP for Masters Course from government of India, India. Merit scholarship from the Chaitanya charity fund for SSLC for securing high marks. Trends-organized by Bangalore University, India Slide show presentation in CFTRI,Mysore,India

Professional Memberships Society of Biological Chemists (India) Mysore Chapter REFEREES 1. Dr. Cletus D’Souza Professor and Chair Department of Biochemistry, University of Mysore Mysore, Karnataka, India PIN - 570006 Tel +91 9448958499 Email [email protected] 2. Dr.Kemparaj Reader Department of Biochemistry, University of Mysore Mysore, Karnataka, INDIA PIN - 570006 Tel +91 9945996543 Email [email protected] 3. Dr. Rashmi Pant Senior Scientist Molecular Connections Pvt ltd Kandala mansions, Basavangudi Bangalore- 560 065 INDIA. Email [email protected]