Methods In Molecular Medicine -vascular Disease- Molecular Biology And Gene Therapy Protocols

3.3 mb, 435 pages methods in molecular medicine vascular disease molecular biology and gene therapy protocols 1 detection of mutations and dna polymorphisms in genes involved in cardiovascular diseases by polymerase chain reaction�single-strand conformation polymorphism analysis 2 analysis of genetic variants in cardiovascular risk genes by heteroduplex analysis 3 radiation hybrid (rh) mapping of human smooth muscle-restricted genes 4 efficient extraction of rna from vascular tissue 5 preparation of cdna libraries from vascular cells 6 screening cdna libraries using partial probes to isolate full-length cdnas from vascular cells 7 cloning full-length cdnas from vascular tissues and cells by rapid amplification of cdna ends (race) and rt-pcr 9 the use of differential mrna display (ddrt-pcr) to identify genes differentially expressed in normal and diseased vascular cells 10 identification of novel protein kinases in vascular cells 11 primer extension analysis to map transcription start sites of vascular genes 12 use of liposome-mediated dna transfection to determine promoter activity in smooth muscle cells 13 nuclear run-on assay to study gene transcription in vascular cells 14 electromobility shift analysis (emsa) applied to the study of nf-kappa b binding interactions in vascular smooth muscle cells 15 measurement of gene expression in the vascular wall by reverse transcriptionpolymerase chain reaction (rt-pcr) analysis 16 northern blot analysis to quantify gene expression in vascular diseases 17 localization of gene expression in human atherosclerotic lesions by in situ hybridization 18 rnase protection assays for quantitation of gene expression in vascular tissue 19 detection of apoptosis in atherosclerosis and restenosis by terminal dutp nickend labeling (tunel) 20 in vitro detection of apoptosis in isolated vascular cells 21 molecular assessment of the phenotypic changes associated with smooth muscle cells using two-dimension electrophoresis and microsequence analysis 22 simple methods for preparing recombinant adenoviruses for high-efficiency transduction of vascular cells 23 generation of recombinant adeno-associated viruses for delivery of genes into vascular cells 24 hemagglutinating virus of japan liposome-mediated gene delivery to vascular cells 25 high-efficiency and low-toxicity adenovirus-assisted endothelial transfection 26 gene transfer in vascular cells using an engineered na-h exchanger (nhe1) as a selectable marker 27 use of retroviruses to express exogenous genes in vascular smooth muscle cells 28 embryonal stem (es) cell�derived macrophages a cellular system that facilitates the genetic dissection of macrophage function 29 delivery of recombinant adenoviruses to human saphenous vein 30 an in vivo angiogenesis assay to study positive and negative regulators of neovascularization 31 efficient liposome-mediated gene transfer to rabbit carotid arteries in vivo 32 gene deliver y to rabbit arteries using the collar model 33 delivery of antisense oligonucleotides to the vascular wall 34 local gene delivery of recombinant adenoviruses to the rat carotid artery in vivo

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