Meeting Minutes 19 October 2009

Meeting minutes 19 October 2009 Microscope oil deposits: Proposals: -

Remove the lens and the responsible would give them to the person using the microscope. No

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Use Xylene, to clean the oil off the lens. (Toxic) Maybe

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Check list for people to follow instructions. Yes

Tibi’s Labview program: -

Computer freezes, we need a master switch for the microscope for emergency. We can buy a new computer? We have to figure out if it is a software issue or computer. Mohammad is in charge of that computer.

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Labview training sessions not necessary? No.

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People working with it are Mohammad, Ben, Nag and Setareh. There is a booklet beside the computer with explanations for the program.

Genome center journal club: -

Mostly proteomics and genomics. High throughput.

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We need to sign up? We have to find out how it works.

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Veronique and Wen will join

Journal authorship: -

Who is author and patent?

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Those having a significant contribution, ex. making a figure for your paper, that’s up for discussions and has to be clearly noted when working with the person.

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David encourages us to open up place for other people by getting them involved.

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Corresponding authorship is usually David if work was done in our lab.

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Patents are the same. Who contributes to the idea? The percentages of contribution are open to discussion. Once the patent is signed the content cannot be changed. At McGill 65% of income belongs to the inventors, the applicant is McGill.

New materials on wiki:

General structure of materials: -

Resources: Left side is detail of resource. Please download manuals locally because in few years the same manual might not be available that that same link. Responsible for each of the resources upload the PDF file.

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Group the internal for Details of the Resources because the page is too big. Split up and make it shorter. Responsible for each section should do that?

Incufridge: -

Incubator useful for multilayer soft lithography needs CO2 source. It also has a cooling function.

Presentations:

Roozbeh: Practice for microTAS presentation this week, 20 minutes. Wednesday lunch. Do we have a room?

Presenters: Veronique, Rym, Ben, Nag, Wen

Veronique: update on project -

Software validation, use data from her data on Array Pro, Suggestion: -David suggested using other proteomics data, -Saule suggested adding the Undo, redoing function, auto save every 10 minutes? Save and reload.

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SDS Page from CSF, 20 µl CSF contains enough proteins to detect.

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10% and 12% acrylamide gel.

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Comparing two samples of patient CSF to see sign of proteolyses. There seems to be none. David suggests: Write down what inhibitor you used.

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Wen proposed to use 2D SDS page.

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The general pattern of proteins is the same which shows that there is no general proteolysis. No need to proteomics inhibitors.

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Red blood cells in sample therefore centrifuge at 4 degrees.

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It is preferable to use plasma rather than serum but plasma is trickier. Plasma is time sensitive because of protein degradation. It would be good to see difference between plasma and serum.

Ben: -

Probe fabrication. NOA 61

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Palladium deposition for high sensitive detection

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UV lamp curing problems because of holes in UV lamp. Need to add a paper under the dish

Rym: -

Validated pins, detachment process was difficult because HF entered and replaced oxide between layers. Yield of 80%.

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Ben: use razor blade to separate?

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Thicker wafer 10um?

Nag: -

The shape of dye can be controlled by height of probe over the organotypic slice.

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We would like to understand the mechanism by which the dye enters the slice, diffusion or convection.

Wen: -

antibodies