GROUP ACTIVITY FOR EXERCISE 3 Objective 2: Identify the characteristics of the major phylogenetic groups of protists Table 1 Major phylogenetic groups, their representatives, and major characteristics GROUP
REPRESENTATIVE
CHARACTERISTICS
Euglena
- unicellular simple flagellates -may be photosynthetic or heterotrophic
Paramecium
- unicellular microorganisms with diverse characteristics in subgroups -unified by the presence of air sacs called alveoli beneath cell surfaces
Stramenopiles: Bacillariophytes
Navicula
-unicellular microorganisms which has intricate skeletons and various symmetrical body organizations -cell walls are called frustules that are made up of pectin and silica; -reproductive cells are motile and flagellated -photosynthetic
Rhodophytes
Galaxaura
-unicellular to multicellular, photosynthetic organisms -red in color -cell walls contain polysaccharides agar and carrageenan -food material is floridean starch
Chlorophytes
Spirogyra, Volvox
-unicellular, colonial, to filamentous green-colored organisms -photosynthetic
Euglenozoans
Alveolates
-motile with various methods of motility Mycetozoans: Myxomycetes
Physarum
-unicellular, plasmodial slime molds -has alternating generation in sporic meiosis (can become amoeba-like and flagellated cells after spore germination)
Stramenopiles: Phaeophytes
Sargassum
-microscopic filamentous to macroscopic seaweeds -brown in color -photosynthetic -cell walls contain large amounts of alginic acid -food materials are polysaccharide laminarin, sugar alcohol mannitol, and lipid droplets -reproductive cells are flagellated
Rhizopods
Amoeba
-unicellular protist that use cytoplasmic extensions called pseudopods for motility and food ingestion
Objective 3: Define terms used in identification and classification of major protistan taxa Objective 4: Recognize representative taxa Table 2.1 The Euglenozoa: Euglena
General information Feature we can derive from its name:
True eyes (but not really)
Cell number:
unicellular
Shape:
Irregular, eggplant, cucumber
Habitat:
Freshwater ponds
Relevant structures/process for:
motility
Specific structure/process flagella
Plant-like or animal-like animal-like
covering
pellicle
animal-like
sensory
eyespot
plant-like
chloroplasts
plant-like
Paramylon body
plant-like
Cytostome, reservoir
animal-like
organelles for food production for food storage vestigial structure Other terms/concepts *Type of movement *Paramylon
-metaboly -carbohydrate
animal-like plant-like
Checklist of structures in label: flagellum | cytostome/gullet | reservoir | eye-spot/stigma | chloroplasts | paramylum bodies | pellicle Table 2.2 The Alveolates: Paramecium
General information Feature we can derive from its name:
Elongated
Cell number:
unicellular
Shape:
slipper-shaped
Habitat:
freshwater
Relevant structures/process for:
Specific structure/process
Plant-like or animal-like
motility
cilia
animal-like
feeding
Cytostome, gullet
animal-like
covering
pellicle
animal-like
genetic material
Macronucleus, micronucleus
animal-like
osmoregulation
Contractile vacuole
animal-like
Other terms/concepts *Type of movement *For slide 2: means of reproduction
-spiral -transverse fission (asexual), conjugation (sexual)
Animal-like animal-like
Checklist of structures in label: cilia | macronucleus | micronucleus | Anterior end | Posterior end | Pellicle | Oral groove | Cytostome | gullet/cytopharynx | food vacuoles | contractile vacuoles & radiating canals
Table 2.3 The Stramenopiles: Navicula
General information Feature we can derive from its name: Cell number:
“boat-shaped” Unicellular
Shape:
Boat
Habitat:
Freshwater or Saltwater
Relevant structures/process for:
motility
Specific structure/process Reproductive cells only (flagellated)
Plant-like or animal-like Animal-like
feeding
Photosynthetic (stored as oils & chrysolaminarin)
Plant-like
covering
Cell Wall (Pectin & Silica)
Plant-like
Frustules - two halves of the cell wall - Hypotheca - inner - Epitheca - outer genetic material
Nucleus
Plant-like and Animal-like
Raphe - main line Striae - branching from raphe Polar Nodules - at both ends of raphe
Plant-like
*Two views
Girdle View - side view Valve View - top view
-
*Symmetry
Bilateral - “winged” (Pennate Diatoms)
-
*Life cycle (type of meiosis)
Diplontic ( Gametic Meiosis)
-
Other terms/concepts *Other structures
Checklist of structures in label: Nucleus | Cell Wall (Hypotheca & Epitheca) | Raphe | Striae | Polar Nodules | Chloroplast
Table 2.4 The Phaeophytes: Sargassum
General Information Feature we can derive from its name:
-
Sargasso – seaweed Phaeo – brown Phyta – algae
Cell Number:
-
Multicellular Filamentous under microscope
Color: Habitat:
Brown Salt water
Relevant Structures/processes for:
Motility
Covering
Specific structure/process Non-motile but floats along currents and waves -
Cellulosic layer Mucilaginous layer
Plant-like or Animal-like Plant-like
Plant-like
Organelles For food production For storage
Chloroplast
Plant-like
-
Laminarin Mannitol Lipid droplets
Plant-like
-
Stipe Holdfast
Plant-like
Blade
Plant-like
For floatation
Air bladder
Plant-like
For reproduction
Receptacle
Plant-like
Cryptostomata
Plant-like
Other structures For support
For food production
Sterile cavities bearing small hairs Other terms/concepts *means of reproduction *type of meiosis *type of life cycle
-
Binary Fission (Asexual) Gametic/Diplon tic cycle (Sexual)
Animal-like -
Table 2.5 The Rhodophytes: Galaxaura
General information Feature we can derive from its name:
Named after an oceanid creature in Greek mythology; organism is found in oceans especially in the benthic region
Cell number:
Multicellular
Shape:
Dichotomous branching
Habitat:
Saltwater
Color:
Red, masking chlorophyll a and d, through presence of biliprotein pigments, r-phycoerythrin (red) and r-phycocyanin (blue)
Motility: Relevant structures/process for:
Non-motile Specific structure/process
Plant-like or animal-like
Feeding
Photosynthetic (Floridean starch)
Plant-like
Covering
Cell Wall (Mucilagenous, Cellulosic, Agar and Carrageenan)
Plant-like
Nucleus
Plant-like and Animal-like
Diplohaplontic ( Zygotic Meiosis)
-
Dichotomous
Plant-like
genetic material
Other terms/concepts Life cycle (type of meiosis)
Type of frond branching
Checklist of structures in label: Cell Membrane | Cell Wall | Mitochondrion | Nucleus | Stellate Chloroplast | Floridean Starch | Vacuole
Table 2.6 The Chlorophytes: Volvox
General Information Feature we can derive from its name Cell Number
“To roll” Unicellular, colonial
Shape
Spherical
Habitat
Freshwater
Color
Green
Motility Relevant structures/processes for: Food production Covering Motility Reproduction
Sensory Other terms/concepts Coenobic - each colony of Volvox h as definite number of daughter cells Protoplasmic strand - connect each individual cell for the colony to act as One
Non-motile Specific structure/process
Plant-like or animal-like
Chlorophyll Photosynthetic
Plant-like
Gelatinous matrix
Animal-like
Flagella
Animal-like
Sexual (ova and spermatozoa) Asexual (gonidia)
Animal-like
eyespots
Animal-like -
Checklist of structures in label: Flagella | Daughter cell | Gelatinous Matrix |Gonidia | Protoplasmic Strands |
Table 2.7 The Chlorophytes: Spirogyra
General information Feature we can derive from its name: Cell number:
Helical or spiral arrangement of chloroplasts Filamentous
Shape:
Cylindrical shape
Habitat:
Freshwater
Relevant structure/process for:
Specific structure/process
Plant-like or animal-like
Cell Wall (Outer: Pectin; inner: Cellulose)
Plant-like
Chloroplast and Pyrenoids
Plant-like
For food storage
Vacuoles
Plant-like
For the storage of DNA
Nucleus
Plant-like and Animal-like
Cytoplasm
Plant-like and Animal-like
Conjugation Tube and Zygospore
-
Covering
Organelles For food production
For cellular process
Other Terms/concepts: *Means of Reproduction
Checklist of structure in label: Filament | Zygospore | Conjugation Tube | Chloroplast | Cell wall | pyrenoid | nucleus | cytoplasm
Table 2.8 The Mycetozoa: Physarum
General information Feature we can derive from its name: Cell number: Shape:
Habitat:
Slimy (snail-like) unicellular Fan-shaped (plasmodium) | mushroom-like (fruiting body) damp, dark places
Relevant structures/process for:
Specific structure/process
Plant-like or animal-like
motility
Plasmodium is motile like amoeba
animal-like
feeding
phagotrophic
animal-like
fruiting body
sporangium
plant-like
spores
plant-like
Spores (sexual) Fission (asexual)
plant-like plant-like
plasmodium sporangium activated spore
plant-like plant-like animal-like
genetic material reproduction
Life cycle Plasmodial form Sporulation Amoeba and Flagellate (swarm cell) Other terms/concepts Movement: amoeboid Survival: scloretia (hardening and drying) Sensory: chemotaxis
Animal-like Animal-like animal-like
Checklist of structure in label: Plasmodium | Sporangium | Sporangiophore | Spore | Swarm Cell
Table 2.9 The Rhizopods: Amoeba
General information Feature we can derive from its name:
Cell number:
Greek amoibē meaning it changes or alters its form unicellular
Shape:
Irregular; has many cytoplasmic extensions
Habitat:
Freshwater, sea, soil, animals, etc.; ubiquitous
Relevant structures/process for:
Specific structure/process
Plant-like or animal-like
motility
Pseudopodia (false feet)
animal-like
feeding
Pseudopodia (cytoplasmic extensions)
animal-like
Other terms/concepts *Two layers of cytoplasm:
- clear ectoplasm (aids in motility and food ingestion) -granular endoplasm (contains the vacuoles, granules, food vesicles, and other organelles)
animal-like
Mode of reproduction:
-binary fission and multiple fission (both asexual only)
animal-like
Checklist of structure in label: Pseudopodia | Endoplasm | Ectoplasm
Objective 5: Explain why protists are the most diverse in terms of metabolism, structure and life cycle Table 3. Summary of similarities and differences among the different major groups GROUP
REPRESENT ATIVE
MOTILITY
FEEDING HABITS
MEANS OF REPRODUCTI ON
Other info
Stramenopiles: Baciliariophyte s
Navicula
Vegetative cells are nonmotile but reproductive cells are flagellated
Photosynthetic
Alveolates
Paramecium
Spiral due to specialized structure called cilia; dinoflagellates have flagella
Phagotrophy
● Ciliates:Trans verse fission (asexual); Conjugation (sexual) ● Apicomplexan s: Multiple fission (asexual); Sporogony (sexual) ● Dinoflagellate s: Binary fission (asexual); Isogamy (sexual)
-possess air sacs called alveoli beneath cell surfaces
Stramenopiles: Phaeophytes
Sargassum
Vegetative cells are nonmotile but reproductive cells are flagellated
Photosynthesi s
Fragmentation, zoospores and aplanospores (asexual); Isogamy, anisogamy, and
-microscopic filamentous to macroscopic seaweeds -brown in color
Shrinking Cell walls are division/MacDon called frustules ald-Pfitzer rule/ and are (asexual); composed of oogamy in the hypotheca centric and (inner covering) isogamy in and epitheca pennate (outer diatoms (sexual) covering) made up of pectin and silica
oogamy (sexual)
due to masking of chlorophyll by xanthophyll pigment--fucox anthin -photosynthetic -cell walls contain large amounts of alginic acid -food materials are polysaccharide laminarin, sugar alcohol mannitol, and lipid droplets
Euglenozoans
Euglena
Metaboly and has a flagellum
Photosynthesi s and some heterotrophic parasites
Longitudinal fission in euglenoids, binary fission in kinetoplastids (asexual); no known sexual mode of reproduction
-flagellated organisms -diverse in modes of nutrition
Rhodophytes
Galaxaura
Non-motile
Photosynthesi s
Aplanospores (asexual); Oogamy (sexual
-photosynthetic red-colored algae which are masked by pigments r-phycoerythrin and r-phycocyanin -food material is called floridean starch -cell walls contain polysaccharide s agar and carrageenan
Chlorophytes
Spirogyra, Volvox
Complex methods of motility
Photosynthesi s
Binary fission, fragmentation, zoospores (asexual); Isogamy, Anisogamy, Oogamy (sexual)
-green-colored photosynthetic organisms -unicellular, colonial, to filamentous forms
Mycetozoans: Myxomycetes
Physarum
Plasmodium state: protoplasmic streaming Swarm cell state: flagella
Phagotrophy
Mitotic division and sporulation (asexual); Nuclear fusion (sexual)
-has alternating generations in sporic meiosis
Rhizopoda
Amoeba
Amoeboid locomotion due to pseudopods
Phagotrophy
Binary fission, multiple fission (asexual); no known sexual mode of reproduction
Flexible endoplasm (granulated membrane) which engulfs its food and releases digestive enzymes.