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CH 395G BIO 395G MOL 395G Fall 2004 Tuesday & Thursday 11-12:30 WEL 2.304 Instructors: Barrie Kitto and Marvin Hackert Teaching Assistant : Larisa Watson
Dr. Marvin Hackert Structural Molecular Biology, Protein Crystallography Enzyme Mechanisms
PLP_Dependent Decarboxylases http://www.cm.utexas.edu/hackert/
Dr Barrie Kitto
Immunoassays
Contraceptives
http://www.cm.utexas.edu/kitto/Kittolabpage/
Course Schedule
Introductory Material Use the class web site at:
cm.utexas.edu/CH395G/Kitto/for all announcements, exam dates, times and place and class Class lecture note key points will also be posted there in PDF (Acrobat) format. Use of University Libraries http://www.lib.utexas.edu/ Chemistry Library WEL 2.132 Life Sciences Library MAI 220 Use of Internet: From laboratories Computer Center WEL 2.302 Limitations of internet Journals on internet Use of CHIME for 3D Structures
college.com/chem/biochem/GarrettGrisham/WebLinks/WebLinks.html
Necessity to use Netscape 4.x as browse
CHIME and 3D Structures • Use Mozilla or Netscape 4.x • See www.boyerbiochem.com for some examples
Lecture 1 Outline Chapter 1 Small to large --- from precursors to cells The interior of cells ---crowded Intracellular location of processes Bioelements Biopolymers Ordering of informatiion
Chapter 2 Aqueous conditions Water -- polar nature Solvent properties Hydrogen bonds Acids and Bases pH Henderson Hasselbalch Equation
Constituents of Cells: E. coli % Water 70 Proteins 15 Nucleic Acids 7 Polysaccharides 3 Lipids 2 Monomers 2 Inorganic Ions 1
Hemoglobin 3 x 108 molecules per cell 5 x 106 cells/ml
Bioelements Water: H,O Organics: C,H,O,N,P,S Ions: Na+, K+, Mg++, Ca++, ClTrace Elements: Fe, Mn ,Co, Cu, Zn
Chapter 2 • Water • Acids and Bases • Ionization
Molarity of Water 1 liter of water weighs 1000 g 1 mole of water = 18 g Therefore the molarity of water is = 1000/18 M = 55M
Acids, Bases and Buffers pH, Titration, pKa, pKb, physiological buffers
Ionization of Water
pH • Because it is cumbersome to use exponentials to describe the amount of hydrogen ions present in a solution the pH scale was adopted. • pH = - log10 [H+] • For example if [H+] = 10-7M the pH = 7
Table 2-3 (bottom) Dissociation Constants and pK’s at 25°C of Some Acids in Common Laboratory Use as
Page 45
Biochemical Buffers
.
For reviews of pH and buffers see http://www.boyerbiochem.com/ and go to Concept Reviews
Dr. Marvin Hackert Structural Molecular Biology, Protein Crystallography Enzyme Mechanisms
PLP_Dependent Decarboxylases http://www.cm.utexas.edu/hackert/
Dr Barrie Kitto
Immunoassays
Contraceptives
http://www.cm.utexas.edu/kitto/Kittolabpage/
Course Schedule
Introductory Material Use the class web site at:
cm.utexas.edu/CH395G/Kitto/for all announcements, exam dates, times and place and class Class lecture note key points will also be posted there in PDF (Acrobat) format. Use of University Libraries http://www.lib.utexas.edu/ Chemistry Library WEL 2.132 Life Sciences Library MAI 220 Use of Internet: From laboratories Computer Center WEL 2.302 Limitations of internet Journals on internet Use of CHIME for 3D Structures
college.com/chem/biochem/GarrettGrisham/WebLinks/WebLinks.html
Necessity to use Netscape 4.x as browse
CHIME and 3D Structures • Use Mozilla or Netscape 4.x • See www.boyerbiochem.com for some examples
Lecture 1 Outline Chapter 1 Small to large --- from precursors to cells The interior of cells ---crowded Intracellular location of processes Bioelements Biopolymers Ordering of informatiion
Chapter 2 Aqueous conditions Water -- polar nature Solvent properties Hydrogen bonds Acids and Bases pH Henderson Hasselbalch Equation
Constituents of Cells: E. coli % Water 70 Proteins 15 Nucleic Acids 7 Polysaccharides 3 Lipids 2 Monomers 2 Inorganic Ions 1
Hemoglobin 3 x 108 molecules per cell 5 x 106 cells/ml
Bioelements Water: H,O Organics: C,H,O,N,P,S Ions: Na+, K+, Mg++, Ca++, ClTrace Elements: Fe, Mn ,Co, Cu, Zn
Chapter 2 • Water • Acids and Bases • Ionization
Molarity of Water 1 liter of water weighs 1000 g 1 mole of water = 18 g Therefore the molarity of water is = 1000/18 M = 55M
Acids, Bases and Buffers pH, Titration, pKa, pKb, physiological buffers
Ionization of Water
pH • Because it is cumbersome to use exponentials to describe the amount of hydrogen ions present in a solution the pH scale was adopted. • pH = - log10 [H+] • For example if [H+] = 10-7M the pH = 7
Table 2-3 (bottom) Dissociation Constants and pK’s at 25°C of Some Acids in Common Laboratory Use as
Page 45
Biochemical Buffers
.
For reviews of pH and buffers see http://www.boyerbiochem.com/ and go to Concept Reviews
